Restriction map for
pFN21AE1904
##################################
# NO CUT ENZYMES ( 138 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AgeI AhdI Alw44I
AlwNI AocI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspHI
AspI AviII AvrII BamHI BanI
BbeI BbrPI BbsI BbvCI BcgI
BcnI BfrI BglI BlnI BlpI
Bpu1102I BpuAI BsaAI BsaHI BsaI
BsaWI BseAI BsiEI BsiHKAI BsiWI
BsmAI BsmBI Bsp1407I BspDI BspEI
BspHI BspMI BsrDI BsrFI BsrGI
BssHII Bst1107I BstAPI BstEII BstPI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DrdI EagI Eam1105I EclXI
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FseI FspI HgaI
Hin1I HindIII HpaI KasI KpnI
KspI MfeI MluI MroI MslI
MspA1I MunI NaeI NarI NciI
NgoMIV NheI NotI NruI NsiI
PacI PflFI PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PstI PvuI PvuII RcaI RsrII
SacI SacII SalI SbfI ScaI
SexAI SfiI SfoI SgrAI SmaI
SnaBI SpeI Sse8387I SspBI SspI
SwaI Tth111I Van91I XbaI XcmI
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AccII 91 CGCG CG^CG
AflIII 1259 ACATGT A^C[GA][CT]GT
Aor51HI 395 AGCGCT AGC^GCT
ApaI 208 GGGCCC GGGCC^C
AvaI 173 CTCGAG C^[CT]CG[GA]G
BciVI 1276 GGATAC GTATCC(6/5)
BclI 318 TGATCA T^GATCA
BglII 714 AGATCT A^GATCT
BpmI 287 CTCCAG CTGGAG(16/14)
BsaBI 1277 GATACAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI 43 GTGCAG GTGCAG(16/14)
BsmFI 1263 GTCCC GGGAC(10/14)
BsmI 24 GAATGC GAATGC(1/-1)
BsoBI 173 CTCGAG C^[CT]CG[GA]G
BspLU11I 1259 ACATGT A^CATGT
BsrBI 188 CCGCTC CCGCTC(-3/-3)
BssSI 491 CACGAG CACGAG(-5/-1)
BstBI 797 TTCGAA TT^CGAA
BstUI 91 CGCG CG^CG
DraI 309 TTTAAA TTT^AAA
DraIII 840 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 366 CCATGG C^C[GA][CT]GG
Eco47III 395 AGCGCT AGC^GCT
FauI 1181 CCCGC CCCGC(4/6)
FbaI 318 TGATCA T^GATCA
HapII 21 CCGG C^CGG
HincII 667 GTCAAC GT[CT]^[GA]AC
HindII 667 GTCAAC GT[CT]^[GA]AC
HpaII 21 CCGG C^CGG
MaeII 561 ACGT A^CGT
MamI 1277 GATACAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspI 21 CCGG C^CGG
MvnI 91 CGCG CG^CG
NcoI 366 CCATGG C^CATGG
NdeI 685 CATATG CA^TATG
NspV 797 TTCGAA TT^CGAA
PaeR7I 173 CTCGAG C^TCGAG
PleI 665 GAGTC GAGTC(4/5)
SapI 1079 GCTCTTC GCTCTTC(1/4)
SfcI 731 CTGTAG C^T[GA][CT]AG
SfuI 797 TTCGAA TT^CGAA
SphI 257 GCATGC GCATG^C
StuI 75 AGGCCT AGG^CCT
XhoI 173 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AciI 188 CCGC CCGC(-3/-1)
1182 CCGC
AlwI 54 GATCC GGATC(4/5)
752 GGATC
AvaII 201 GGACC G^G[AT]CC
738 GGACC
BanII 208 GGGCCC G[GA]GC[CT]^C
778 GAGCCC
BbvI 1157 GCAGC GCAGC(8/12)
517 GCTGC
BmyI 208 GGGCCC G[AGT]GC[ACT]^C
778 GAGCCC
Bsp1286I 208 GGGCCC G[AGT]GC[ACT]^C
778 GAGCCC
BstXI 619 CCAGGGAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1266 CCAAAAAGATGG
CfoI 130 GCGC GCG^C
396 GCGC
EcoT14I 330 CCTTGG C^C[AT][AT]GG
366 CCATGG
HaeII 129 GGCGCT [GA]GCGC^[CT]
395 AGCGCT
HhaI 130 GCGC GCG^C
396 GCGC
HinP1I 130 GCGC G^CGC
396 GCGC
MseI 310 TTAA T^TAA
425 TTAA
NspI 257 GCATGC [GA]CATG^[CT]
1259 ACATGT
PpuMI 200 AGGACCC [GA]G^G[AT]CC[CT]
737 AGGACCT
SmlI 173 CTCGAG C^T[CT][GA]AG
772 CTCAAG
StyI 330 CCTTGG C^C[AT][AT]GG
366 CCATGG
Tru9I 310 TTAA T^TAA
425 TTAA
TseI 517 GCTGC G^C[AT]GC
1157 GCAGC
TspRI 242 CACTG CA[GC]TG[ACGT][ACGT]^
729 CACTG
#########################################
# 3 OR MORE CUT ENZYMES ( 55 enzymes) #
#########################################
AcsI AfaI AluI ApoI BalI
BfaI BsaJI BseRI BsiYI BslI
BsrI BssKI BstF5I BstNI BstYI
Cac8I Cfr13I DdeI DpnII DraII
EaeI EarI EcoO109I EcoRII Fnu4HI
FokI HaeIII HinfI HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MflI MluNI MnlI MscI MvaI
MwoI NdeII NlaIII NlaIV RsaI
Sau3AI Sau96I ScrFI SfaNI TaqI
TfiI Tsp45I Tsp509I TthHB8I XhoII