Restriction map for
pFN21AE1821
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# NO CUT ENZYMES ( 128 enzymes) #
##################################
Acc65I AccI AclI AgeI AhdI
AlwNI AocI ApaI AscI AseI
AsnI Asp700I Asp718I AspEI AvaII
AviII AvrII BanI BbeI BbrPI
BbsI BbvCI BcgI BciVI BcnI
BfaI BglI BglII BlnI BpuAI
BsaAI BsaBI BsaI BsiEI BsiWI
BsmBI BsmI Bsp1407I BspDI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstXI BstZ17I Bsu36I Cfr10I ClaI
CpoI DraIII DrdI DsaI EagI
Eam1105I EclXI Eco52I Eco81I EcoO65I
EcoRI EcoRV EcoT22I FseI FspI
HgaI HincII HindII HpaI KasI
KpnI KspI MaeI MamI MfeI
MunI NaeI NarI NciI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PinAI PleI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI RsrII SacI
SacII SalI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I SspBI SspI
StuI SwaI TspRI XbaI XcmI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 68 enzymes) #
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AatII 561 GACGTC GACGT^C
AccII 203 CGCG CG^CG
AccIII 790 TCCGGA T^CCGGA
AcsI 322 AAATTT [GA]^AATT[CT]
AcyI 561 GACGTC G[GA]^CG[CT]C
AfaI 831 GTAC GT^AC
AflII 42 CTTAAG C^TTAAG
Alw44I 856 GTGCAC G^TGCAC
Aor51HI 971 AGCGCT AGC^GCT
ApaLI 856 GTGCAC G^TGCAC
ApoI 322 AAATTT [GA]^AATT[CT]
AspHI 856 GTGCAC G[AT]GC[AT]^C
AspI 493 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 440 CTCGAG C^[CT]CG[GA]G
BamHI 384 GGATCC G^GATCC
BanII 362 GAGCCC G[GA]GC[CT]^C
BclI 327 TGATCA T^GATCA
BfrI 42 CTTAAG C^TTAAG
BlpI 757 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 757 GCTGAGC GC^T[ACGT]AGC
BsaHI 561 GACGTC G[GA]^CG[CT]C
BseAI 790 TCCGGA T^CCGGA
BsgI 770 CTGCAC GTGCAG(16/14)
BsiHKAI 856 GTGCAC G[AT]GC[AT]^C
BsmAI 794 GAGAC GTCTC(1/5)
BsmFI 59 GGGAC GGGAC(10/14)
BsoBI 440 CTCGAG C^[CT]CG[GA]G
BspEI 790 TCCGGA T^CCGGA
BspHI 936 TCATGA T^CATGA
BspLU11I 827 ACATGT A^CATGT
BsrI 77 ACTGG ACTGG(1/-1)
BstUI 203 CGCG CG^CG
CelII 757 GCTGAGC GC^T[ACGT]AGC
CfoI 972 GCGC GCG^C
Cfr13I 51 GGCCC G^G[ACGT]CC
DraI 610 TTTAAA TTT^AAA
DraII 50 AGGCCCT [GA]G^G[ACGT]CC[CT]
EarI 940 GAAGAG CTCTTC(1/4)
Eco47III 971 AGCGCT AGC^GCT
EcoO109I 50 AGGCCCT [GA]G^G[ACGT]CC[CT]
FauI 639 CCCGC CCCGC(4/6)
FbaI 327 TGATCA T^GATCA
HaeII 971 AGCGCT [GA]GCGC^[CT]
HhaI 972 GCGC GCG^C
Hin1I 561 GACGTC G[GA]^CG[CT]C
HinP1I 972 GCGC G^CGC
Ksp632I 940 GAAGAG CTCTTC(1/4)
MluI 202 ACGCGT A^CGCGT
MroI 790 TCCGGA T^CCGGA
MslI 423 CACAATGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 518 CAGCTG C[AC]G^C[GT]G
MvnI 203 CGCG CG^CG
PaeR7I 440 CTCGAG C^TCGAG
PflFI 493 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 969 CCAGCGCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 946 CTGCAG CTGCA^G
PvuII 518 CAGCTG CAG^CTG
RcaI 936 TCATGA T^CATGA
RsaI 831 GTAC GT^AC
SapI 940 GAAGAGC GCTCTTC(1/4)
Sau96I 51 GGCCC G^G[ACGT]CC
SfcI 946 CTGCAG C^T[GA][CT]AG
SphI 784 GCATGC GCATG^C
TaqI 441 TCGA T^CGA
Tth111I 493 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 441 TCGA T^CGA
Van91I 969 CCAGCGCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 440 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AflIII 202 ACGCGT A^C[GA][CT]GT
827 ACATGT
BalI 191 TGGCCA TGG^CCA
253 TGGCCA
BmyI 362 GAGCCC G[AGT]GC[ACT]^C
856 GTGCAC
BpmI 695 CTCCAG CTGGAG(16/14)
22 CTGGAG
BsaWI 790 TCCGGA [AT]^CCGG[AT]
819 TCCGGT
BseRI 121 GAGGAG GAGGAG(10/8)
505 GAGGAG
Bsp1286I 362 GAGCCC G[AGT]GC[ACT]^C
856 GTGCAC
BspMI 804 ACCTGC ACCTGC(4/8)
852 GCAGGT
BstF5I 870 CATCC GGATG(2/0)
928 CATCC
BstYI 241 GGATCT [GA]^GATC[CT]
384 GGATCC
DdeI 357 CTCAG C^T[ACGT]AG
758 CTGAG
EcoNI 250 CCTTGGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
352 CCTCTCTCAGG
FokI 870 CATCC GGATG(9/13)
928 CATCC
HapII 791 CCGG C^CGG
820 CCGG
HpaII 791 CCGG C^CGG
820 CCGG
MflI 241 GGATCT [GA]^GATC[CT]
384 GGATCC
MluNI 191 TGGCCA TGG^CCA
253 TGGCCA
MscI 191 TGGCCA TGG^CCA
253 TGGCCA
MspI 791 CCGG C^CGG
820 CCGG
NspI 784 GCATGC [GA]CATG^[CT]
827 ACATGT
SmlI 42 CTTAAG C^T[CT][GA]AG
440 CTCGAG
Tsp509I 323 AATT ^AATT
371 AATT
XhoII 241 GGATCT [GA]^GATC[CT]
384 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 39 enzymes) #
#########################################
AciI AluI AlwI BbvI BsaJI
BsiYI BslI BssKI BstNI Cac8I
DpnII EaeI EcoRII EcoT14I Fnu4HI
HaeIII HindIII HinfI HphI ItaI
MaeII MaeIII MboI MboII MnlI
MseI MvaI MwoI NdeII NlaIII
NlaIV Sau3AI ScrFI SfaNI StyI
TfiI Tru9I TseI Tsp45I