##################################
# NO CUT ENZYMES (  199 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AcyI        AflII       AgeI        AhdI        
Alw44I      AlwI        AlwNI       AocI        Aor51HI     
ApaI        ApaLI       ApoI        AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AspI        
AvaI        AviII       AvrII       BalI        BamHI       
BanI        BbeI        BbrPI       BbsI        BbvCI       
BcgI        BclI        BcnI        BfrI        BglI        
BglII       BlnI        BlpI        BpmI        Bpu1102I    
BpuAI       BsaAI       BsaBI       BsaHI       BsaI        
BsaWI       BseAI       BsgI        BsiEI       BsiWI       
BsmAI       BsmBI       BsmFI       BsoBI       BspDI       
BspEI       BspHI       BspLU11I    BspMI       BsrBI       
BsrDI       BsrFI       BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstF5I      BstPI       
BstYI       BstZ17I     Bsu36I      CelII       CfoI        
Cfr10I      ClaI        CpoI        DpnII       DraI        
DraII       DraIII      DrdI        DsaI        EaeI        
EagI        Eam1105I    EarI        EclXI       Eco47III    
Eco52I      Eco81I      EcoO109I    EcoO65I     EcoRI       
EcoRV       EcoT22I     FbaI        FokI        FseI        
FspI        HaeII       HapII       HgaI        HhaI        
Hin1I       HinP1I      HincII      HindII      HindIII     
HinfI       HpaI        HpaII       HphI        KasI        
KpnI        Ksp632I     KspI        MaeIII      MamI        
MboI        MfeI        MflI        MluI        MluNI       
MroI        MscI        MspA1I      MspI        MunI        
NaeI        NarI        NciI        NcoI        NdeI        
NdeII       NgoMIV      NheI        NotI        NruI        
NsiI        NspI        NspV        PacI        PaeR7I      
PflFI       PflMI       PinAI       PleI        PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
Psp1406I    PstI        PvuI        PvuII       RcaI        
RsrII       SacI        SacII       SalI        SapI        
Sau3AI      SbfI        ScaI        SexAI       SfcI        
SfiI        SfoI        SfuI        SgrAI       SmaI        
SmlI        SnaBI       SpeI        SphI        Sse8387I    
SspI        StuI        SwaI        TfiI        Tsp45I      
Tsp509I     Tth111I     Van91I      XbaI        XhoI        
XhoII       XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   27 enzymes) #
###################################

AccII         248  CGCG                 CG^CG
AflIII        188  ACACGT               A^C[GA][CT]GT
AspHI         390  GTGCTC               G[AT]GC[AT]^C
BseRI         341  GAGGAG               GAGGAG(10/8)
BsiHKAI       390  GTGCTC               G[AT]GC[AT]^C
BsmI          167  GCATTC               GAATGC(1/-1)
Bsp1407I      128  TGTACA               T^GTACA
BsrGI         128  TGTACA               T^GTACA
BsrI           40  ACTGG                ACTGG(1/-1)
BstUI         248  CGCG                 CG^CG
BstXI         402  CCAGCATCTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DdeI           14  CTAAG                C^T[ACGT]AG
EcoNI           9  CCTCTCTAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I        89  CCAAGG               C^C[AT][AT]GG
MaeII         190  ACGT                 A^CGT
MboII         158  TCTTC                GAAGA(8/7)
MseI          419  TTAA                 T^TAA
MslI          381  CACAACTGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI          248  CGCG                 CG^CG
NlaIII        126  CATG                 CATG^
SfaNI         405  GCATC                GCATC(5/9)
SspBI         128  TGTACA               T^GTACA
StyI           89  CCAAGG               C^C[AT][AT]GG
TaqI          339  TCGA                 T^CGA
Tru9I         419  TTAA                 T^TAA
TspRI         435  CAGTG                CA[GC]TG[ACGT][ACGT]^
TthHB8I       339  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (   14 enzymes) #
###################################

AciI           96  CCGC                 CCGC(-3/-1)
              247  CCGC                
AfaI          105  GTAC                 GT^AC
              129  GTAC                
BanII         176  GAGCCC               G[GA]GC[CT]^C
              344  GAGCCC              
BbvI           46  GCTGC                GCAGC(8/12)
              449  GCTGC               
BciVI         117  GTATCC               GTATCC(6/5)
              201  GTATCC              
BfaI           73  CTAG                 C^TAG
              223  CTAG                
FauI           95  CCCGC                CCCGC(4/6)
              246  CCCGC               
Fnu4HI         46  GCTGC                GC^[ACGT]GC
              449  GCTGC               
ItaI           46  GCTGC                GC^[ACGT]GC
              449  GCTGC               
MaeI           73  CTAG                 C^TAG
              223  CTAG                
MwoI           67  GCCCACCTAGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              371  GCTTGGTGGGC         
RsaI          105  GTAC                 GT^AC
              129  GTAC                
TseI           46  GCTGC                G^C[AT]GC
              449  GCTGC               
XcmI           50  CCAGCTTTATAATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              356  CCATCGCCCTCCTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   18 enzymes) #
#########################################

AluI        AvaII       BmyI        BsaJI       BsiYI       
BslI        Bsp1286I    BssKI       BstNI       Cac8I       
Cfr13I      EcoRII      HaeIII      MnlI        MvaI        
NlaIV       Sau96I      ScrFI