Restriction map for
pFN21AE3314
##################################
# NO CUT ENZYMES ( 109 enzymes) #
##################################
Acc65I AccI AccII AccIII AclI
AgeI AhdI Alw44I Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvaI AviII
AvrII BbsI BbvCI BcgI BlnI
BpuAI BseAI BsgI BsiWI BsmBI
BsoBI BspDI BspEI BspHI BsrFI
BssHII Bst1107I BstBI BstEII BstPI
BstUI BstZ17I Cfr10I ClaI CpoI
DraI DrdI EagI Eam1105I EclXI
Eco47III Eco52I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FseI FspI HgaI
HindIII HpaI KpnI KspI MluI
MroI MvnI NaeI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmeI
PshAI PshBI Psp1406I RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspI StuI SwaI Tth111I XbaI
XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AatII 976 GACGTC GACGT^C
AflII 1867 CTTAAG C^TTAAG
AocI 1543 CCTGAGG CC^T[ACGT]AGG
BanII 2008 GAGCCC G[GA]GC[CT]^C
BbeI 311 GGCGCC GGCGC^C
BbrPI 745 CACGTG CAC^GTG
BciVI 664 GGATAC GTATCC(6/5)
BclI 1955 TGATCA T^GATCA
BfrI 1867 CTTAAG C^TTAAG
BglI 1449 GCCATCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 2484 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2484 GCTCAGC GC^T[ACGT]AGC
BsaAI 745 CACGTG [CT]AC^GT[GA]
BsaI 2417 GAGACC GGTCTC(1/5)
BsiEI 1413 CGATCG CG[GA][CT]^CG
BspLU11I 869 ACATGT A^CATGT
BspMI 1637 ACCTGC ACCTGC(4/8)
BsrDI 1105 GCAATG GCAATG(2/0)
BssSI 2005 CACGAG CACGAG(-5/-1)
BstAPI 1275 GCAGAAAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 1530 CCAGCCCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1543 CCTGAGG CC^T[ACGT]AGG
CelII 2484 GCTCAGC GC^T[ACGT]AGC
CfoI 312 GCGC GCG^C
DraIII 1382 CACCTAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 1631 CCATGG C^C[GA][CT]GG
EarI 2498 GAAGAG CTCTTC(1/4)
Eco81I 1543 CCTGAGG CC^T[ACGT]AGG
FauI 2000 CCCGC CCCGC(4/6)
FbaI 1955 TGATCA T^GATCA
HaeII 311 GGCGCC [GA]GCGC^[CT]
HhaI 312 GCGC GCG^C
HinP1I 312 GCGC G^CGC
HincII 979 GTCAAC GT[CT]^[GA]AC
HindII 979 GTCAAC GT[CT]^[GA]AC
KasI 311 GGCGCC G^GCGCC
Ksp632I 2498 GAAGAG CTCTTC(1/4)
MfeI 691 CAATTG C^AATTG
MunI 691 CAATTG C^AATTG
NarI 311 GGCGCC GG^CGCC
NcoI 1631 CCATGG C^CATGG
PmaCI 745 CACGTG CAC^GTG
PmlI 745 CACGTG CAC^GTG
PvuI 1413 CGATCG CGAT^CG
SfoI 311 GGCGCC GGC^GCC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AcyI 311 GGCGCC G[GA]^CG[CT]C
976 GACGTC
AflIII 746 ACGTGT A^C[GA][CT]GT
869 ACATGT
AspHI 989 GTGCTC G[AT]GC[AT]^C
2242 GTGCTC
BamHI 1196 GGATCC G^GATCC
2225 GGATCC
BcnI 754 CCCGG CC^[GC]GG
2235 CCGGG
BsaBI 1875 GATAAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1981 GATCTCAATC
BsaHI 311 GGCGCC G[GA]^CG[CT]C
976 GACGTC
BsaWI 491 TCCGGT [AT]^CCGG[AT]
2222 ACCGGA
BsiHKAI 989 GTGCTC G[AT]GC[AT]^C
2242 GTGCTC
BsmFI 301 GGGAC GGGAC(10/14)
2516 GTCCC
BsmI 17 GAATGC GAATGC(1/-1)
1848 GAATGC
BsrBI 918 CCGCTC CCGCTC(-3/-3)
1371 CCGCTC
Cac8I 773 GCTGGC GC[ACGT]^[ACGT]GC
1620 GCCTGC
DraII 301 GGGACCC [GA]G^G[ACGT]CC[CT]
1898 AGGACCC
EcoO109I 301 GGGACCC [GA]G^G[ACGT]CC[CT]
1898 AGGACCC
Hin1I 311 GGCGCC G[GA]^CG[CT]C
976 GACGTC
MaeII 746 ACGT A^CGT
977 ACGT
MamI 1875 GATAAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1981 GATCTCAATC
NciI 754 CCCGG CC^[GC]GG
2235 CCGGG
PpuMI 301 GGGACCC [GA]G^G[AT]CC[CT]
1898 AGGACCC
PstI 259 CTGCAG CTGCA^G
1506 CTGCAG
TaqI 335 TCGA T^CGA
1405 TCGA
TthHB8I 335 TCGA T^CGA
1405 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 81 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AvaII BalI BanI
BbvI BfaI BglII BmyI BpmI
BsaJI BseRI BsiYI BslI BsmAI
Bsp1286I Bsp1407I BsrGI BsrI BssKI
BstF5I BstNI BstYI Cfr13I DdeI
DpnII EaeI EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HinfI HpaII
HphI ItaI MaeI MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MwoI NdeII NlaIII NlaIV NspI
PflMI PleI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspBI StyI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI Van91I XcmI
XhoII