Restriction map for
pFN21AE2353
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# NO CUT ENZYMES ( 151 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AflII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AspI AviII
AvrII BamHI BbeI BbrPI BbsI
BbvCI BcgI BciVI BclI BcnI
BfrI BlnI BlpI Bpu1102I BpuAI
BsaAI BsaBI BsaWI BseAI BsgI
BsiEI BsiHKAI BsiWI BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrFI
BssHII Bst1107I BstBI BstEII BstPI
BstUI BstZ17I Bsu36I CelII CfoI
Cfr10I ClaI CpoI DraI DraII
DraIII DsaI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HaeII HhaI HinP1I
HindIII HpaI KasI KpnI KspI
MamI MfeI MluI MroI MunI
MvnI NaeI NarI NciI NcoI
NdeI NgoMIV NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SacII SalI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspI StuI SwaI
Tth111I XbaI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AcyI 514 GGCGTC G[GA]^CG[CT]C
AflIII 72 ACACGT A^C[GA][CT]GT
AlwI 798 GATCC GGATC(4/5)
AlwNI 13 CAGTTTCTG CAG[ACGT][ACGT][ACGT]^CTG
AvaI 287 CTCGGG C^[CT]CG[GA]G
AvaII 212 GGTCC G^G[AT]CC
BalI 654 TGGCCA TGG^CCA
BanI 447 GGTGCC G^G[CT][GA]CC
BglI 380 GCCCAGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 95 AGATCT A^GATCT
BsaHI 514 GGCGTC G[GA]^CG[CT]C
BsaI 571 GGTCTC GGTCTC(1/5)
BseRI 817 GAGGAG GAGGAG(10/8)
BsmBI 75 CGTCTC CGTCTC(1/5)
BsmI 366 GCATTC GAATGC(1/-1)
BsoBI 287 CTCGGG C^[CT]CG[GA]G
Bsp1407I 597 TGTACA T^GTACA
BsrDI 230 GCAATG GCAATG(2/0)
BsrGI 597 TGTACA T^GTACA
BssSI 125 CACGAG CACGAG(-5/-1)
BstAPI 12 GCAGTTTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 616 CCAGAAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 95 AGATCT [GA]^GATC[CT]
DrdI 848 GACACAGCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 654 TGGCCA [CT]^GGCC[GA]
FauI 352 GCGGG CCCGC(4/6)
HapII 683 CCGG C^CGG
Hin1I 514 GGCGTC G[GA]^CG[CT]C
HincII 109 GTCAAC GT[CT]^[GA]AC
HindII 109 GTCAAC GT[CT]^[GA]AC
HpaII 683 CCGG C^CGG
MaeII 74 ACGT A^CGT
MflI 95 AGATCT [GA]^GATC[CT]
MluNI 654 TGGCCA TGG^CCA
MscI 654 TGGCCA TGG^CCA
MslI 502 CATCAGTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 658 CAGCTG C[AC]G^C[GT]G
MspI 683 CCGG C^CGG
NheI 24 GCTAGC G^CTAGC
PleI 162 GACTC GAGTC(4/5)
PstI 10 CTGCAG CTGCA^G
PvuII 658 CAGCTG CAG^CTG
SapI 28 GCTCTTC GCTCTTC(1/4)
SfcI 10 CTGCAG C^T[GA][CT]AG
SspBI 597 TGTACA T^GTACA
XcmI 694 CCAAAGCCTGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 95 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 26 enzymes) #
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AciI 352 GCGG CCGC(-3/-1)
720 GCGG
AfaI 340 GTAC GT^AC
598 GTAC
BanII 135 GGGCTC G[GA]GC[CT]^C
378 GAGCCC
BbvI 21 GCTGC GCAGC(8/12)
363 GCTGC
BpmI 455 CTCCAG CTGGAG(16/14)
802 CTGGAG
Cfr13I 212 GGTCC G^G[ACGT]CC
474 GGCCC
DpnII 96 GATC ^GATC
798 GATC
EarI 29 CTCTTC CTCTTC(1/4)
235 GAAGAG
Fnu4HI 21 GCTGC GC^[ACGT]GC
363 GCTGC
HgaI 58 GACGC GACGC(5/10)
515 GCGTC
ItaI 21 GCTGC GC^[ACGT]GC
363 GCTGC
Ksp632I 29 CTCTTC CTCTTC(1/4)
235 GAAGAG
MboI 96 GATC ^GATC
798 GATC
MseI 283 TTAA T^TAA
968 TTAA
NdeII 96 GATC ^GATC
798 GATC
NlaIV 447 GGTGCC GG[ACGT]^[ACGT]CC
819 GGAGCC
PflMI 457 CCAGGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
902 CCATGAATTGG
RsaI 340 GTAC GT^AC
598 GTAC
Sau3AI 96 GATC ^GATC
798 GATC
Sau96I 212 GGTCC G^G[ACGT]CC
474 GGCCC
TaqI 322 TCGA T^CGA
840 TCGA
Tru9I 283 TTAA T^TAA
968 TTAA
TseI 21 GCTGC G^C[AT]GC
363 GCTGC
Tsp509I 195 AATT ^AATT
907 AATT
TthHB8I 322 TCGA T^CGA
840 TCGA
Van91I 457 CCAGGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
902 CCATGAATTGG
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# 3 OR MORE CUT ENZYMES ( 34 enzymes) #
#########################################
AluI BfaI BmyI BsaJI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrI
BssKI BstF5I BstNI Cac8I DdeI
EcoRII EcoT14I FokI HaeIII HinfI
HphI MaeI MaeIII MboII MnlI
MvaI MwoI NlaIII ScrFI SfaNI
StyI TfiI Tsp45I TspRI