Restriction map for
pFN21AE1822
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# NO CUT ENZYMES ( 141 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AfaI AflII AflIII AgeI AhdI
AlwI AlwNI AocI ApaI AscI
AseI AsnI Asp718I AspEI AvaI
AviII AvrII BamHI BbeI BbrPI
BbsI BbvCI BciVI BclI BfrI
BglI BlnI BlpI Bpu1102I BpuAI
BsaAI BsaBI BsaI BsaWI BseAI
BsiEI BsiWI BsmI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstXI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EarI EclXI Eco52I Eco81I
EcoNI EcoO65I EcoT22I FbaI FseI
FspI HincII HindII HindIII HpaI
KasI KpnI Ksp632I KspI MaeIII
MamI MfeI MluI MroI MunI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI RcaI RsaI RsrII SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI Tsp45I XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 54 enzymes) #
###################################
AccII 125 CGCG CG^CG
AcsI 301 GAATTC [GA]^AATT[CT]
AcyI 997 GGCGTC G[GA]^CG[CT]C
Alw44I 364 GTGCAC G^TGCAC
Aor51HI 52 AGCGCT AGC^GCT
ApaLI 364 GTGCAC G^TGCAC
ApoI 301 GAATTC [GA]^AATT[CT]
Asp700I 297 GAAGGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII 212 GGTCC G^G[AT]CC
BalI 482 TGGCCA TGG^CCA
BanII 337 GAGCTC G[GA]GC[CT]^C
BcgI 255 GCATATATTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 968 CCCGG CC^[GC]GG
BfaI 856 CTAG C^TAG
BglII 95 AGATCT A^GATCT
BsaHI 997 GGCGTC G[GA]^CG[CT]C
BsgI 526 GTGCAG GTGCAG(16/14)
BsmBI 244 GAGACG CGTCTC(1/5)
BspMI 547 ACCTGC ACCTGC(4/8)
BsrBI 195 CCGCTC CCGCTC(-3/-3)
BstUI 125 CGCG CG^CG
BstYI 95 AGATCT [GA]^GATC[CT]
DpnII 96 GATC ^GATC
DraII 461 AGGCCCC [GA]G^G[ACGT]CC[CT]
Eco47III 52 AGCGCT AGC^GCT
EcoO109I 461 AGGCCCC [GA]G^G[ACGT]CC[CT]
EcoRI 301 GAATTC G^AATTC
EcoRV 418 GATATC GAT^ATC
HapII 969 CCGG C^CGG
Hin1I 997 GGCGTC G[GA]^CG[CT]C
HpaII 969 CCGG C^CGG
MaeI 856 CTAG C^TAG
MboI 96 GATC ^GATC
MflI 95 AGATCT [GA]^GATC[CT]
MluNI 482 TGGCCA TGG^CCA
MscI 482 TGGCCA TGG^CCA
MspI 969 CCGG C^CGG
MvnI 125 CGCG CG^CG
NciI 968 CCCGG CC^[GC]GG
NcoI 902 CCATGG C^CATGG
NdeII 96 GATC ^GATC
PflMI 614 CCAAGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SacI 337 GAGCTC GAGCT^C
Sau3AI 96 GATC ^GATC
SfaNI 714 GATGC GCATC(5/9)
SmlI 574 CTTGAG C^T[CT][GA]AG
StuI 617 AGGCCT AGG^CCT
TaqI 273 TCGA T^CGA
TfiI 683 GATTC G^A[AT]TC
TthHB8I 273 TCGA T^CGA
Van91I 614 CCAAGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 855 TCTAGA T^CTAGA
XhoII 95 AGATCT [GA]^GATC[CT]
XmnI 297 GAAGGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AspI 187 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
868 GACATCGTC
BanI 5 GGTGCC G^G[CT][GA]CC
544 GGCACC
BseRI 34 CTCCTC GAGGAG(10/8)
946 CTCCTC
BsrI 881 ACTGG ACTGG(1/-1)
638 CCAGT
DsaI 630 CCGTGG C^C[GA][CT]GG
902 CCATGG
EaeI 482 TGGCCA [CT]^GGCC[GA]
651 TGGCCG
FauI 63 CCCGC CCCGC(4/6)
717 GCGGG
HaeII 30 GGCGCT [GA]GCGC^[CT]
52 AGCGCT
MaeII 223 ACGT A^CGT
293 ACGT
MboII 597 GAAGA GAAGA(8/7)
392 TCTTC
MseI 539 TTAA T^TAA
974 TTAA
MslI 486 CATTCAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
897 CACCTCCATG
PflFI 187 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
868 GACATCGTC
PleI 181 GACTC GAGTC(4/5)
577 GAGTC
PstI 157 CTGCAG CTGCA^G
250 CTGCAG
SfcI 157 CTGCAG C^T[GA][CT]AG
250 CTGCAG
Tru9I 539 TTAA T^TAA
974 TTAA
Tsp509I 302 AATT ^AATT
557 AATT
Tth111I 187 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
868 GACATCGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 44 enzymes) #
#########################################
AciI AluI AspHI BbvI BmyI
BpmI BsaJI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BssKI BstF5I
BstNI Cac8I CfoI Cfr13I DdeI
EcoRII EcoT14I Fnu4HI FokI HaeIII
HgaI HhaI HinP1I HinfI HphI
ItaI MnlI MspA1I MvaI MwoI
NlaIII NlaIV PvuII Sau96I ScrFI
StyI TseI TspRI XcmI