Restriction map for
pFN21AE2094
##################################
# NO CUT ENZYMES ( 97 enzymes) #
##################################
AatII AccIII AclI AcsI AflII
AgeI AocI ApoI AscI AseI
AsnI AspI AviII AvrII BbvCI
BcgI BciVI BclI BfrI BlnI
BsaBI BsaWI BseAI BsiEI BsiWI
BsmI Bsp1407I BspDI BspEI BspLU11I
BsrDI BsrGI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstZ17I Bsu36I
ClaI CpoI DraI DrdI EagI
EclXI Eco52I Eco81I EcoO65I EcoRI
EcoT22I FbaI FseI FspI HindIII
HpaI KspI MamI MfeI MluI
MroI MseI MunI NdeI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmeI PshAI PshBI
Psp1406I PvuI RsrII SacII SalI
SbfI ScaI SexAI SfiI SfuI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI Tru9I Tsp509I Tth111I
XbaI XhoI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
Acc65I 2183 GGTACC G^GTACC
AccII 19 CGCG CG^CG
AflIII 2126 ACGTGT A^C[GA][CT]GT
AhdI 1982 GACTGGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 479 AGCGCT AGC^GCT
Asp700I 1566 GAAGGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 2183 GGTACC G^GTACC
AspEI 1982 GACTGGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 680 GGATCC G^GATCC
BbrPI 2246 CACGTG CAC^GTG
BbsI 1570 GTCTTC GAAGAC(2/6)
BglI 122 GCCCCCACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 2156 AGATCT A^GATCT
BlpI 1681 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1681 GCTGAGC GC^T[ACGT]AGC
BpuAI 1570 GTCTTC GAAGAC(2/6)
BsaAI 2246 CACGTG [CT]AC^GT[GA]
BspHI 1403 TCATGA T^CATGA
BspMI 182 ACCTGC ACCTGC(4/8)
BsrBI 2239 GAGCGG CCGCTC(-3/-3)
BstUI 19 CGCG CG^CG
BstXI 1823 CCAAGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 1681 GCTGAGC GC^T[ACGT]AGC
DraIII 1711 CACATCGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 1982 GACTGGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 479 AGCGCT AGC^GCT
EcoRV 2020 GATATC GAT^ATC
KpnI 2183 GGTACC GGTAC^C
MvnI 19 CGCG CG^CG
NcoI 1809 CCATGG C^CATGG
NheI 1891 GCTAGC G^CTAGC
NspI 554 ACATGC [GA]CATG^[CT]
PleI 382 GAGTC GAGTC(4/5)
PmaCI 2246 CACGTG CAC^GTG
PmlI 2246 CACGTG CAC^GTG
RcaI 1403 TCATGA T^CATGA
SapI 945 GCTCTTC GCTCTTC(1/4)
SgrAI 1187 CGCCGGTG C[GA]^CCGG[CT]G
SmaI 152 CCCGGG CCC^GGG
SmlI 1534 CTCAAG C^T[CT][GA]AG
StuI 1106 AGGCCT AGG^CCT
TfiI 360 GAATC G^A[AT]TC
XcmI 602 CCAATGCCTCGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 152 CCCGGG C^CCGGG
XmaI 152 CCCGGG C^CCGGG
XmnI 1566 GAAGGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AccI 2266 GTCTAC GT^[AC][GT]AC
2368 GTCTAC
Alw44I 1939 GTGCAC G^TGCAC
2329 GTGCAC
ApaI 161 GGGCCC GGGCC^C
541 GGGCCC
ApaLI 1939 GTGCAC G^TGCAC
2329 GTGCAC
BfaI 1892 CTAG C^TAG
1969 CTAG
BsmBI 843 CGTCTC CGTCTC(1/5)
932 CGTCTC
BstYI 680 GGATCC [GA]^GATC[CT]
2156 AGATCT
EcoNI 663 CCTGGAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2349 CCTGGCCCAGG
EcoT14I 1458 CCAAGG C^C[AT][AT]GG
1809 CCATGG
HgaI 1645 GCGTC GACGC(5/10)
1730 GCGTC
HincII 838 GTCAAC GT[CT]^[GA]AC
1060 GTCAAC
HindII 838 GTCAAC GT[CT]^[GA]AC
1060 GTCAAC
HinfI 360 GAATC G^A[ACGT]TC
382 GAGTC
MaeI 1892 CTAG C^TAG
1969 CTAG
MflI 680 GGATCC [GA]^GATC[CT]
2156 AGATCT
SacI 208 GAGCTC GAGCT^C
247 GAGCTC
StyI 1458 CCAAGG C^C[AT][AT]GG
1809 CCATGG
XhoII 680 GGATCC [GA]^GATC[CT]
2156 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 97 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwI
AlwNI AspHI AvaI AvaII BalI
BanI BanII BbeI BbvI BcnI
BmyI BpmI BsaHI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BsrFI
BsrI BssKI BstAPI BstF5I BstNI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoO109I EcoRII FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI Hin1I
HinP1I HpaII HphI ItaI KasI
Ksp632I MaeII MaeIII MboI MboII
MluNI MnlI MscI MslI MspA1I
MspI MvaI MwoI NaeI NarI
NciI NdeII NgoMIV NlaIII NlaIV
PflMI PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SfoI TaqI TseI Tsp45I TspRI
TthHB8I Van91I