Restriction map for
pFN21AE2255
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# NO CUT ENZYMES ( 133 enzymes) #
##################################
AatII Acc65I AccI AccII AclI
AflII AflIII AgeI AhdI Alw44I
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AspI AviII
AvrII BamHI BbeI BbrPI BbvCI
BciVI BclI BfrI BglII BlnI
BpmI BsaAI BsaBI BsaI BseRI
BsgI BsiHKAI BsiWI BsmBI BsmI
Bsp1407I BspDI BspHI BspLU11I BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstUI BstZ17I
Cfr10I ClaI CpoI DraI DraIII
EagI Eam1105I EclXI Eco52I EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HincII HindII HindIII HpaI
KasI KpnI KspI MamI MfeI
MluI MseI MunI MvnI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshBI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SapI SbfI SfiI SfoI
SfuI SgrAI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI TfiI Tru9I Tth111I Van91I
XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 54 enzymes) #
###################################
AccIII 55 TCCGGA T^CCGGA
AcyI 652 GGCGTC G[GA]^CG[CT]C
AlwI 975 GGATC GGATC(4/5)
Aor51HI 399 AGCGCT AGC^GCT
ApaI 470 GGGCCC GGGCC^C
AvaII 258 GGACC G^G[AT]CC
BalI 428 TGGCCA TGG^CCA
BcgI 292 GCAGCCGAGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 106 CTAG C^TAG
BlpI 402 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 402 GCTGAGC GC^T[ACGT]AGC
BsaHI 652 GGCGTC G[GA]^CG[CT]C
BsaWI 55 TCCGGA [AT]^CCGG[AT]
BseAI 55 TCCGGA T^CCGGA
BsiEI 982 CGGTCG CG[GA][CT]^CG
BsmAI 929 GTCTC GTCTC(1/5)
BspEI 55 TCCGGA T^CCGGA
BsrBI 735 CCGCTC CCGCTC(-3/-3)
BstAPI 899 GCAATAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 747 CATCC GGATG(2/0)
BstXI 782 CCAACCATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 975 GGATCT [GA]^GATC[CT]
CelII 402 GCTGAGC GC^T[ACGT]AGC
DpnII 976 GATC ^GATC
DraII 604 GGGGCCT [GA]G^G[ACGT]CC[CT]
DsaI 431 CCATGG C^C[GA][CT]GG
EarI 463 CTCTTC CTCTTC(1/4)
Eco47III 399 AGCGCT AGC^GCT
EcoNI 1029 CCTAAAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 604 GGGGCCT [GA]G^G[ACGT]CC[CT]
FokI 747 CATCC GGATG(9/13)
HaeII 399 AGCGCT [GA]GCGC^[CT]
HgaI 653 GCGTC GACGC(5/10)
Hin1I 652 GGCGTC G[GA]^CG[CT]C
HinfI 298 GAGTC G^A[ACGT]TC
Ksp632I 463 CTCTTC CTCTTC(1/4)
MaeI 106 CTAG C^TAG
MboI 976 GATC ^GATC
MflI 975 GGATCT [GA]^GATC[CT]
MluNI 428 TGGCCA TGG^CCA
MroI 55 TCCGGA T^CCGGA
MscI 428 TGGCCA TGG^CCA
MslI 747 CATCCTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI 431 CCATGG C^CATGG
NdeII 976 GATC ^GATC
PleI 298 GAGTC GAGTC(4/5)
PshAI 861 GACGTATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Sau3AI 976 GATC ^GATC
ScaI 841 AGTACT AGT^ACT
SexAI 824 ACCTGGT A^CC[AT]GGT
TaqI 985 TCGA T^CGA
TthHB8I 985 TCGA T^CGA
XcmI 152 CCATTGGCTTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 975 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AcsI 100 AAATTC [GA]^AATT[CT]
168 AAATTT
AlwNI 591 CAGGTACTG CAG[ACGT][ACGT][ACGT]^CTG
952 CAGGCTCTG
AocI 111 CCTGAGG CC^T[ACGT]AGG
933 CCTGAGG
ApoI 100 AAATTC [GA]^AATT[CT]
168 AAATTT
AvaI 1067 CCCGGG C^[CT]CG[GA]G
1117 CCCGGG
BglI 295 GCCGAGTCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
481 GCCACCCTGGC
BsmFI 264 GGGAC GGGAC(10/14)
1111 GGGAC
BsoBI 1067 CCCGGG C^[CT]CG[GA]G
1117 CCCGGG
Bsu36I 111 CCTGAGG CC^T[ACGT]AGG
933 CCTGAGG
CfoI 400 GCGC GCG^C
564 GCGC
DrdI 229 GACACCTTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
718 GACAGCAGGGTC
EaeI 356 CGGCCA [CT]^GGCC[GA]
428 TGGCCA
Eco81I 111 CCTGAGG CC^T[ACGT]AGG
933 CCTGAGG
EcoT14I 431 CCATGG C^C[AT][AT]GG
647 CCTTGG
HhaI 400 GCGC GCG^C
564 GCGC
HinP1I 400 GCGC G^CGC
564 GCGC
MaeII 371 ACGT A^CGT
862 ACGT
PstI 616 CTGCAG CTGCA^G
691 CTGCAG
PvuII 527 CAGCTG CAG^CTG
613 CAGCTG
SfcI 616 CTGCAG C^T[GA][CT]AG
691 CTGCAG
SmaI 1067 CCCGGG CCC^GGG
1117 CCCGGG
StyI 431 CCATGG C^C[AT][AT]GG
647 CCTTGG
Tsp509I 101 AATT ^AATT
169 AATT
XmaCI 1067 CCCGGG C^CCGGG
1117 CCCGGG
XmaI 1067 CCCGGG C^CCGGG
1117 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 46 enzymes) #
#########################################
AciI AfaI AluI BanI BanII
BbsI BbvI BcnI BmyI BpuAI
BsaJI BsiYI BslI Bsp1286I BspMI
BsrI BssKI BstNI Cac8I Cfr13I
DdeI EcoRII FauI Fnu4HI HaeIII
HapII HpaII HphI ItaI MaeIII
MboII MnlI MspA1I MspI MvaI
MwoI NciI NlaIII NlaIV RsaI
Sau96I ScrFI SfaNI TseI Tsp45I
TspRI