Restriction map for
pFN21AE5268
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# NO CUT ENZYMES ( 156 enzymes) #
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AatII Acc65I AccIII AclI AflII
AflIII AgeI AhdI Alw44I AlwI
AlwNI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AvaII AviII
AvrII BamHI BbeI BbrPI BbvCI
BciVI BclI BfrI BglI BglII
BlnI BlpI Bpu1102I BsaBI BsaI
BsaWI BseAI BsgI BsiEI BsiWI
BsmI BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrDI BsrFI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstYI BstZ17I Cac8I CelII
Cfr10I ClaI CpoI DpnII DraI
DraIII DrdI EagI Eam1105I EarI
EclXI Eco52I EcoNI EcoO65I EcoRV
EcoT14I EcoT22I FbaI FseI FspI
HincII HindII HindIII HpaI HphI
KasI KpnI Ksp632I KspI MamI
MboI MfeI MflI MluI MroI
MseI MspA1I MunI NaeI NarI
NcoI NdeI NdeII NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI PvuII RcaI
RsrII SacI SacII SalI SapI
Sau3AI SbfI ScaI SexAI SfaNI
SfcI SfiI SfoI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspI
StuI StyI SwaI Tru9I Tsp45I
Van91I XbaI XcmI XhoI XhoII
XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AccI 88 GTCTAC GT^[AC][GT]AC
AcsI 596 GAATTC [GA]^AATT[CT]
AcyI 127 GACGCC G[GA]^CG[CT]C
AfaI 225 GTAC GT^AC
AluI 140 AGCT AG^CT
AocI 199 CCTGAGG CC^T[ACGT]AGG
ApoI 596 GAATTC [GA]^AATT[CT]
AspHI 294 GTGCTC G[AT]GC[AT]^C
AspI 163 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanI 402 GGTGCC G^G[CT][GA]CC
BbsI 587 GTCTTC GAAGAC(2/6)
BcgI 210 CGAGACGTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 160 CTAG C^TAG
BpuAI 587 GTCTTC GAAGAC(2/6)
BsaAI 82 CACGTA [CT]AC^GT[GA]
BsaHI 127 GACGCC G[GA]^CG[CT]C
BseRI 122 GAGGAG GAGGAG(10/8)
BsiHKAI 294 GTGCTC G[AT]GC[AT]^C
BsmFI 533 GGGAC GGGAC(10/14)
Bsp1407I 224 TGTACA T^GTACA
BsrGI 224 TGTACA T^GTACA
BstF5I 484 CATCC GGATG(2/0)
BstXI 483 CCATCCTGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 199 CCTGAGG CC^T[ACGT]AGG
DsaI 131 CCGTGG C^C[GA][CT]GG
Eco81I 199 CCTGAGG CC^T[ACGT]AGG
EcoRI 596 GAATTC G^AATTC
FauI 62 GCGGG CCCGC(4/6)
FokI 484 CATCC GGATG(9/13)
HapII 273 CCGG C^CGG
HgaI 127 GACGC GACGC(5/10)
Hin1I 127 GACGCC G[GA]^CG[CT]C
HpaII 273 CCGG C^CGG
MaeI 160 CTAG C^TAG
MaeIII 104 GTAAC ^GT[ACGT]AC
MspI 273 CCGG C^CGG
PflFI 163 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
RsaI 225 GTAC GT^AC
SmaI 272 CCCGGG CCC^GGG
SmlI 432 CTTGAG C^T[CT][GA]AG
SspBI 224 TGTACA T^GTACA
TaqI 120 TCGA T^CGA
TspRI 427 CAGTG CA[GC]TG[ACGT][ACGT]^
Tth111I 163 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 120 TCGA T^CGA
XmaCI 272 CCCGGG C^CCGGG
XmaI 272 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AccII 39 CGCG CG^CG
61 CGCG
Aor51HI 284 AGCGCT AGC^GCT
338 AGCGCT
AvaI 208 CCCGAG C^[CT]CG[GA]G
272 CCCGGG
BcnI 272 CCCGG CC^[GC]GG
273 CCGGG
BsmBI 125 GAGACG CGTCTC(1/5)
211 GAGACG
BsoBI 208 CCCGAG C^[CT]CG[GA]G
272 CCCGGG
BstUI 39 CGCG CG^CG
61 CGCG
Eco47III 284 AGCGCT AGC^GCT
338 AGCGCT
HaeII 284 AGCGCT [GA]GCGC^[CT]
338 AGCGCT
MaeII 83 ACGT A^CGT
214 ACGT
MslI 396 CATCTCGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
515 CATCAGGGTG
MvnI 39 CGCG CG^CG
61 CGCG
NciI 272 CCCGG CC^[GC]GG
273 CCGGG
NlaIII 228 CATG CATG^
313 CATG
PleI 250 GAGTC GAGTC(4/5)
475 GAGTC
TfiI 333 GATTC G^A[AT]TC
579 GATTC
Tsp509I 320 AATT ^AATT
597 AATT
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# 3 OR MORE CUT ENZYMES ( 38 enzymes) #
#########################################
AciI ApaI BalI BanII BbvI
BmyI BpmI BsaJI BsiYI BslI
BsmAI Bsp1286I BsrI BssKI BstNI
CfoI Cfr13I DdeI DraII EaeI
EcoO109I EcoRII Fnu4HI HaeIII HhaI
HinP1I HinfI ItaI MboII MluNI
MnlI MscI MvaI MwoI NlaIV
Sau96I ScrFI TseI