Restriction map for
pFN21AE4196
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# NO CUT ENZYMES ( 88 enzymes) #
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AatII Acc65I AclI AflII AgeI
AhdI Alw44I Aor51HI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII AvrII BclI BfrI
BglII BlnI BlpI Bpu1102I BsaBI
BsiEI BsiWI BsmI BspHI BspLU11I
BssHII BssSI Bst1107I BstEII BstPI
BstZ17I CelII CpoI DraI DraIII
EagI Eam1105I EclXI Eco47III Eco52I
EcoO65I EcoRI EcoT22I FbaI FseI
FspI HindIII HpaI KpnI MamI
MfeI MluI MunI NdeI NotI
NruI NsiI PacI PaeR7I PflFI
PinAI PmeI PshAI PshBI Psp1406I
PvuI RcaI RsrII SbfI SexAI
SfiI SgrAI SmaI SmlI SnaBI
Sse8387I SwaI Tth111I XbaI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 62 enzymes) #
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AccI 702 GTCGAC GT^[AC][GT]AC
AccIII 1721 TCCGGA T^CCGGA
AcsI 988 GAATTT [GA]^AATT[CT]
ApoI 988 GAATTT [GA]^AATT[CT]
BalI 179 TGGCCA TGG^CCA
BamHI 1769 GGATCC G^GATCC
BbeI 116 GGCGCC GGCGC^C
BbrPI 1132 CACGTG CAC^GTG
BbsI 726 GAAGAC GAAGAC(2/6)
BbvCI 1303 GCTGAGG CCTCAGC(-5/-2)
BcgI 2097 CGACGACACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 119 GCCAGCCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 726 GAAGAC GAAGAC(2/6)
BsaI 187 GGTCTC GGTCTC(1/5)
BsaWI 1721 TCCGGA [AT]^CCGG[AT]
BseAI 1721 TCCGGA T^CCGGA
Bsp1407I 248 TGTACA T^GTACA
BspDI 1249 ATCGAT AT^CGAT
BspEI 1721 TCCGGA T^CCGGA
BsrBI 1906 CCGCTC CCGCTC(-3/-3)
BsrFI 1928 GCCGGC [GA]^CCGG[CT]
BsrGI 248 TGTACA T^GTACA
BstAPI 1131 GCACGTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 1550 TTCGAA TT^CGAA
BstYI 1769 GGATCC [GA]^GATC[CT]
Cfr10I 1928 GCCGGC [GA]^CCGG[CT]
ClaI 1249 ATCGAT AT^CGAT
DrdI 1959 GACGCCCGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 179 TGGCCA [CT]^GGCC[GA]
EcoNI 1695 CCTGTTACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 1885 GATATC GAT^ATC
HincII 702 GTCGAC GT[CT]^[GA]AC
HindII 702 GTCGAC GT[CT]^[GA]AC
KasI 116 GGCGCC G^GCGCC
KspI 1896 CCGCGG CCGC^GG
MflI 1769 GGATCC [GA]^GATC[CT]
MluNI 179 TGGCCA TGG^CCA
MroI 1721 TCCGGA T^CCGGA
MscI 179 TGGCCA TGG^CCA
MseI 8 TTAA T^TAA
NaeI 1928 GCCGGC GCC^GGC
NarI 116 GGCGCC GG^CGCC
NgoMIV 1928 GCCGGC G^CCGGC
NheI 1536 GCTAGC G^CTAGC
NspV 1550 TTCGAA TT^CGAA
PmaCI 1132 CACGTG CAC^GTG
PmlI 1132 CACGTG CAC^GTG
PvuII 771 CAGCTG CAG^CTG
SacII 1896 CCGCGG CCGC^GG
SalI 702 GTCGAC G^TCGAC
ScaI 1481 AGTACT AGT^ACT
SfoI 116 GGCGCC GGC^GCC
SfuI 1550 TTCGAA TT^CGAA
SpeI 318 ACTAGT A^CTAGT
SphI 1167 GCATGC GCATG^C
SspBI 248 TGTACA T^GTACA
SspI 1000 AATATT AAT^ATT
StuI 1346 AGGCCT AGG^CCT
TfiI 1597 GAATC G^A[AT]TC
Tru9I 8 TTAA T^TAA
XcmI 963 CCAGTCTATTCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 1769 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 23 enzymes) #
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AcyI 116 GGCGCC G[GA]^CG[CT]C
1959 GACGCC
AflIII 1085 ACGTGT A^C[GA][CT]GT
1973 ACGTGT
AlwI 1770 GATCC GGATC(4/5)
1769 GGATC
AlwNI 161 CAGGCGCTG CAG[ACGT][ACGT][ACGT]^CTG
196 CAGCTCCTG
AocI 625 CCTGAGG CC^T[ACGT]AGG
1839 CCTGAGG
ApaI 1115 GGGCCC GGGCC^C
1565 GGGCCC
AvaI 1954 CCCGAG C^[CT]CG[GA]G
1963 CCCGAG
BciVI 364 GGATAC GTATCC(6/5)
630 GGATAC
BfaI 319 CTAG C^TAG
1537 CTAG
BsaHI 116 GGCGCC G[GA]^CG[CT]C
1959 GACGCC
BsmBI 610 GAGACG CGTCTC(1/5)
1957 GAGACG
BsoBI 1954 CCCGAG C^[CT]CG[GA]G
1963 CCCGAG
BspMI 524 ACCTGC ACCTGC(4/8)
842 ACCTGC
BsrDI 882 CATTGC GCAATG(2/0)
1361 GCAATG
Bsu36I 625 CCTGAGG CC^T[ACGT]AGG
1839 CCTGAGG
Eco81I 625 CCTGAGG CC^T[ACGT]AGG
1839 CCTGAGG
Hin1I 116 GGCGCC G[GA]^CG[CT]C
1959 GACGCC
MaeI 319 CTAG C^TAG
1537 CTAG
NcoI 272 CCATGG C^CATGG
635 CCATGG
SacI 1375 GAGCTC GAGCT^C
2065 GAGCTC
SapI 2062 GAAGAGC GCTCTTC(1/4)
145 GCTCTTC
Tsp45I 139 GTCAC ^GT[GC]AC
2087 GTGAC
Tsp509I 542 AATT ^AATT
989 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 85 enzymes) #
#########################################
AccII AciI AfaI AluI AspHI
AvaII BanI BanII BbvI BcnI
BmyI BpmI BsaAI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI BstUI BstXI Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EarI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeII MaeIII
MboI MboII MnlI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PflMI
PleI PpuMI PstI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI StyI
TaqI TseI TspRI TthHB8I Van91I