Restriction map for
pFN21AE9283
##################################
# NO CUT ENZYMES ( 163 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp718I AspEI AspHI
AvaI AviII AvrII BalI BamHI
BanII BbrPI BbsI BbvCI BcgI
BciVI BclI BfaI BfrI BglII
BlnI BlpI BmyI Bpu1102I BpuAI
BsaAI BsaBI BsaI BsaWI BseAI
BsgI BsiEI BsiHKAI BsiWI BsmBI
BsmI BsoBI Bsp1286I Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstYI
BstZ17I Bsu36I Cac8I CelII Cfr10I
ClaI DraI DraII DraIII DrdI
EaeI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HincII HindII HindIII
HpaI KpnI Ksp632I MaeI MaeII
MamI MfeI MflI MluI MluNI
MroI MscI MseI MslI MunI
NaeI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI PvuII RcaI SacI SalI
SapI SbfI ScaI SexAI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI TfiI
Tru9I Van91I XbaI XcmI XhoI
XhoII XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AcsI 556 GAATTT [GA]^AATT[CT]
AcyI 16 GGCGCC G[GA]^CG[CT]C
AfaI 31 GTAC GT^AC
AluI 102 AGCT AG^CT
AlwI 228 GATCC GGATC(4/5)
AlwNI 523 CAGCGCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 556 GAATTT [GA]^AATT[CT]
Asp700I 63 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 493 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanI 16 GGCGCC G^G[CT][GA]CC
BbeI 16 GGCGCC GGCGC^C
BcnI 59 CCGGG CC^[GC]GG
BsaHI 16 GGCGCC G[GA]^CG[CT]C
BsmAI 180 GAGAC GTCTC(1/5)
BsmFI 485 GGGAC GGGAC(10/14)
BsrBI 564 CCGCTC CCGCTC(-3/-3)
BstF5I 342 CATCC GGATG(2/0)
BstXI 305 CCATCTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CpoI 383 CGGTCCG CG^G[AT]CCG
DpnII 228 GATC ^GATC
EcoT14I 510 CCATGG C^C[AT][AT]GG
FokI 342 CATCC GGATG(9/13)
HgaI 375 GACGC GACGC(5/10)
Hin1I 16 GGCGCC G[GA]^CG[CT]C
HphI 315 GGTGA GGTGA(8/7)
KasI 16 GGCGCC G^GCGCC
KspI 22 CCGCGG CCGC^GG
MboI 228 GATC ^GATC
MboII 279 GAAGA GAAGA(8/7)
NarI 16 GGCGCC GG^CGCC
NciI 59 CCGGG CC^[GC]GG
NcoI 510 CCATGG C^CATGG
NdeII 228 GATC ^GATC
PflFI 493 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PstI 85 CTGCAG CTGCA^G
RsaI 31 GTAC GT^AC
RsrII 383 CGGTCCG CG^G[AT]CCG
SacII 22 CCGCGG CCGC^GG
Sau3AI 228 GATC ^GATC
SfcI 85 CTGCAG C^T[GA][CT]AG
SfiI 360 GGCCTATGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 16 GGCGCC GGC^GCC
SmlI 139 CTCAAG C^T[CT][GA]AG
StyI 510 CCATGG C^C[AT][AT]GG
TaqI 503 TCGA T^CGA
Tsp509I 557 AATT ^AATT
TspRI 346 CAGTG CA[GC]TG[ACGT][ACGT]^
Tth111I 493 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 503 TCGA T^CGA
XmnI 63 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AccII 23 CGCG CG^CG
388 CGCG
AvaII 133 GGTCC G^G[AT]CC
384 GGTCC
BbvI 417 GCAGC GCAGC(8/12)
84 GCTGC
BglI 303 GCCCATCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
361 GCCTATGGGGC
BpmI 567 CTCCAG CTGGAG(16/14)
529 CTGGAG
BseRI 241 GAGGAG GAGGAG(10/8)
532 GAGGAG
BsiYI 7 CCGCTGGGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
21 CCCGCGGCTGG
BslI 7 CCGCTGGGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
21 CCCGCGGCTGG
BsrI 494 ACTGG ACTGG(1/-1)
345 CCAGT
BstUI 23 CGCG CG^CG
388 CGCG
DdeI 155 CTCAG C^T[ACGT]AG
455 CTGAG
DsaI 22 CCGCGG C^C[GA][CT]GG
510 CCATGG
HapII 59 CCGG C^CGG
123 CCGG
HinfI 182 GACTC G^A[ACGT]TC
457 GAGTC
HpaII 59 CCGG C^CGG
123 CCGG
MspI 59 CCGG C^CGG
123 CCGG
MvnI 23 CGCG CG^CG
388 CGCG
PleI 182 GACTC GAGTC(4/5)
457 GAGTC
SfaNI 451 GATGC GCATC(5/9)
293 GCATC
StuI 209 AGGCCT AGG^CCT
359 AGGCCT
TseI 84 GCTGC G^C[AT]GC
417 GCAGC
#########################################
# 3 OR MORE CUT ENZYMES ( 24 enzymes) #
#########################################
AciI BsaJI BssKI BstNI CfoI
Cfr13I EcoRII FauI Fnu4HI HaeII
HaeIII HhaI HinP1I ItaI MaeIII
MnlI MspA1I MvaI MwoI NlaIII
NlaIV Sau96I ScrFI Tsp45I