Restriction map for
pFN21AE4413
##################################
# NO CUT ENZYMES ( 142 enzymes) #
##################################
AatII AccI AccIII AclI AcsI
AcyI AflII AgeI AhdI Alw44I
AocI Aor51HI ApaLI ApoI AscI
AseI AsnI Asp700I AspEI AspHI
AspI AviII AvrII BalI BamHI
BbeI BbvCI BcgI BciVI BfrI
BglII BlnI BlpI Bpu1102I BsaBI
BsaHI BseAI BsiEI BsiHKAI BsiWI
BsmBI BspDI BspEI BspLU11I BspMI
BsrBI BsrFI BssHII Bst1107I BstAPI
BstBI BstEII BstPI BstYI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DraI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT14I EcoT22I
FseI FspI HaeII Hin1I HincII
HindII HindIII HpaI KasI KspI
MamI MfeI MflI MluI MluNI
MroI MscI MunI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PmeI
PpuMI PshAI PshBI Psp1406I PvuI
PvuII RsrII SacI SacII SalI
SbfI ScaI SexAI SfoI SfuI
SgrAI SmlI SnaBI SphI Sse8387I
SspI StuI StyI SwaI TfiI
Tsp509I Tth111I Van91I XbaI XhoI
XhoII XmnI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
Acc65I 533 GGTACC G^GTACC
AccII 135 CGCG CG^CG
AflIII 100 ACACGT A^C[GA][CT]GT
AlwNI 1144 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 398 GGGCCC GGGCC^C
Asp718I 533 GGTACC G^GTACC
BanII 398 GGGCCC G[GA]GC[CT]^C
BbrPI 101 CACGTG CAC^GTG
BclI 83 TGATCA T^GATCA
BfaI 557 CTAG C^TAG
BglI 703 GCCGTGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 101 CACGTG [CT]AC^GT[GA]
BsaI 6 GAGACC GGTCTC(1/5)
BsaWI 1025 ACCGGA [AT]^CCGG[AT]
BsmI 792 GAATGC GAATGC(1/-1)
Bsp1407I 176 TGTACA T^GTACA
BsrDI 501 CATTGC GCAATG(2/0)
BsrGI 176 TGTACA T^GTACA
BssSI 475 CTCGTG CACGAG(-5/-1)
BstUI 135 CGCG CG^CG
BstXI 959 CCAACACTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DsaI 704 CCGTGG C^C[GA][CT]GG
EaeI 701 TGGCCG [CT]^GGCC[GA]
FbaI 83 TGATCA T^GATCA
KpnI 533 GGTACC GGTAC^C
MaeI 557 CTAG C^TAG
MspA1I 1105 CCGCTG C[AC]G^C[GT]G
MvnI 135 CGCG CG^CG
PmaCI 101 CACGTG CAC^GTG
PmlI 101 CACGTG CAC^GTG
PstI 797 CTGCAG CTGCA^G
SfcI 797 CTGCAG C^T[GA][CT]AG
SfiI 702 GGCCGTGGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SpeI 556 ACTAGT A^CTAGT
SspBI 176 TGTACA T^GTACA
Tsp45I 950 GTGAC ^GT[GC]AC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AlwI 497 GGATC GGATC(4/5)
785 GGATC
AvaII 512 GGACC G^G[AT]CC
972 GGACC
BbsI 438 GAAGAC GAAGAC(2/6)
1033 GAAGAC
BpuAI 438 GAAGAC GAAGAC(2/6)
1033 GAAGAC
BseRI 658 CTCCTC GAGGAG(10/8)
918 GAGGAG
BsmAI 6 GAGAC GTCTC(1/5)
656 GTCTC
BsmFI 617 GGGAC GGGAC(10/14)
384 GTCCC
DdeI 770 CTCAG C^T[ACGT]AG
946 CTGAG
DraII 397 GGGGCCC [GA]G^G[ACGT]CC[CT]
398 GGGCCCT
EarI 115 CTCTTC CTCTTC(1/4)
367 CTCTTC
EcoO109I 397 GGGGCCC [GA]G^G[ACGT]CC[CT]
398 GGGCCCT
FauI 386 CCCGC CCCGC(4/6)
425 CCCGC
HgaI 441 GACGC GACGC(5/10)
923 GCGTC
Ksp632I 115 CTCTTC CTCTTC(1/4)
367 CTCTTC
MseI 826 TTAA T^TAA
1006 TTAA
MslI 97 CACACACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
963 CACTCTGGTG
SapI 114 GCTCTTC GCTCTTC(1/4)
366 GCTCTTC
SmaI 275 CCCGGG CCC^GGG
1169 CCCGGG
Tru9I 826 TTAA T^TAA
1006 TTAA
XmaCI 275 CCCGGG C^CCGGG
1169 CCCGGG
XmaI 275 CCCGGG C^CCGGG
1169 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 59 enzymes) #
#########################################
AciI AfaI AluI AvaI BanI
BbvI BcnI BmyI BpmI BsaJI
BsgI BsiYI BslI BsoBI Bsp1286I
BspHI BsrI BssKI BstF5I BstNI
Cac8I CfoI Cfr13I DpnII EcoRII
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
MaeII MaeIII MboI MboII MnlI
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PleI RcaI RsaI
Sau3AI Sau96I ScrFI SfaNI TaqI
TseI TspRI TthHB8I XcmI