Restriction map for
pFN21AE3183
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# NO CUT ENZYMES ( 117 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AflII AgeI AhdI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AviII AvrII BbrPI
BcgI BciVI BfrI BglII BlnI
BsaAI BsaBI BsaI BsaWI BseAI
BsiEI BsiWI BsmBI BsmFI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrGI BssHII Bst1107I
BstBI BstEII BstPI BstZ17I ClaI
DraIII DrdI EagI Eam1105I EclXI
Eco52I EcoO65I EcoRI EcoRV EcoT22I
FseI FspI HgaI HindIII HpaI
KpnI MaeIII MamI MfeI MluI
MroI MunI NaeI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
RcaI SalI SbfI ScaI SexAI
SfcI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI TfiI Tsp45I Tth111I Van91I
XbaI XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AcyI 69 GGCGCC G[GA]^CG[CT]C
AfaI 1242 GTAC GT^AC
AflIII 551 ACGTGT A^C[GA][CT]GT
Alw44I 537 GTGCAC G^TGCAC
ApaLI 537 GTGCAC G^TGCAC
AvaII 262 GGACC G^G[AT]CC
BalI 680 TGGCCA TGG^CCA
BamHI 297 GGATCC G^GATCC
BbeI 69 GGCGCC GGCGC^C
BclI 1106 TGATCA T^GATCA
BfaI 688 CTAG C^TAG
BlpI 204 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 204 GCTGAGC GC^T[ACGT]AGC
BsaHI 69 GGCGCC G[GA]^CG[CT]C
BsmAI 1005 GTCTC GTCTC(1/5)
BsrDI 180 CATTGC GCAATG(2/0)
BsrFI 761 ACCGGC [GA]^CCGG[CT]
BstAPI 308 GCATCCACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 1046 CCATCTGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 204 GCTGAGC GC^T[ACGT]AGC
Cfr10I 761 ACCGGC [GA]^CCGG[CT]
CpoI 261 CGGACCG CG^G[AT]CCG
DraI 961 TTTAAA TTT^AAA
EcoNI 116 CCTGCCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 1211 CCTTGG C^C[AT][AT]GG
FauI 247 GCGGG CCCGC(4/6)
FbaI 1106 TGATCA T^GATCA
Hin1I 69 GGCGCC G[GA]^CG[CT]C
HincII 877 GTTGAC GT[CT]^[GA]AC
HindII 877 GTTGAC GT[CT]^[GA]AC
KasI 69 GGCGCC G^GCGCC
KspI 159 CCGCGG CCGC^GG
MaeI 688 CTAG C^TAG
MaeII 551 ACGT A^CGT
MluNI 680 TGGCCA TGG^CCA
MscI 680 TGGCCA TGG^CCA
NarI 69 GGCGCC GG^CGCC
PaeR7I 142 CTCGAG C^TCGAG
RsaI 1242 GTAC GT^AC
RsrII 261 CGGACCG CG^G[AT]CCG
SacI 775 GAGCTC GAGCT^C
SacII 159 CCGCGG CCGC^GG
SapI 625 GAAGAGC GCTCTTC(1/4)
SfiI 231 GGCCGGAGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 69 GGCGCC GGC^GCC
SmaI 102 CCCGGG CCC^GGG
SmlI 142 CTCGAG C^T[CT][GA]AG
StyI 1211 CCTTGG C^C[AT][AT]GG
Tsp509I 696 AATT ^AATT
XhoI 142 CTCGAG C^TCGAG
XmaCI 102 CCCGGG C^CCGGG
XmaI 102 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 26 enzymes) #
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AccII 160 CGCG CG^CG
255 CGCG
AocI 120 CCTGAGG CC^T[ACGT]AGG
583 CCTGAGG
Aor51HI 170 AGCGCT AGC^GCT
889 AGCGCT
ApaI 99 GGGCCC GGGCC^C
112 GGGCCC
AvaI 102 CCCGGG C^[CT]CG[GA]G
142 CTCGAG
BanI 69 GGCGCC G^G[CT][GA]CC
288 GGTGCC
BbvCI 487 CCTCAGC CCTCAGC(-5/-2)
634 GCTGAGG
BglI 15 GCCGCAGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
232 GCCGGAGGGGC
BseRI 868 CTCCTC GAGGAG(10/8)
574 GAGGAG
BsoBI 102 CCCGGG C^[CT]CG[GA]G
142 CTCGAG
BsrI 1128 ACTGG ACTGG(1/-1)
1009 CCAGT
BstUI 160 CGCG CG^CG
255 CGCG
Bsu36I 120 CCTGAGG CC^T[ACGT]AGG
583 CCTGAGG
DsaI 159 CCGCGG C^C[GA][CT]GG
277 CCACGG
EaeI 162 CGGCCA [CT]^GGCC[GA]
680 TGGCCA
EarI 447 GAAGAG CTCTTC(1/4)
625 GAAGAG
Eco47III 170 AGCGCT AGC^GCT
889 AGCGCT
Eco81I 120 CCTGAGG CC^T[ACGT]AGG
583 CCTGAGG
HphI 1131 GGTGA GGTGA(8/7)
1064 TCACC
Ksp632I 447 GAAGAG CTCTTC(1/4)
625 GAAGAG
MvnI 160 CGCG CG^CG
255 CGCG
NlaIII 1053 CATG CATG^
1110 CATG
PvuII 49 CAGCTG CAG^CTG
632 CAGCTG
TaqI 143 TCGA T^CGA
827 TCGA
TthHB8I 143 TCGA T^CGA
827 TCGA
XcmI 185 CCAGCGCCTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
350 CCATCTGGTCGGTGG
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# 3 OR MORE CUT ENZYMES ( 63 enzymes) #
#########################################
AciI AluI AlwI AlwNI AspHI
BanII BbsI BbvI BcnI BmyI
BpmI BpuAI BsaJI BsgI BsiHKAI
BsiYI BslI Bsp1286I BssKI BssSI
BstF5I BstNI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII EcoO109I
EcoRII Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HinfI HpaII
ItaI MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIV PleI
Sau3AI Sau96I ScrFI SfaNI Tru9I
TseI TspRI XhoII