################################## # NO CUT ENZYMES ( 171 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcyI AflII AgeI Alw44I AlwNI AocI Aor51HI ApaLI AscI AseI AsnI Asp700I Asp718I AspI AvaI AviII AvrII BalI BamHI BanI BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsiEI BsiWI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrFI BsrGI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstF5I BstPI BstXI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraI DraIII DrdI DsaI EaeI EagI EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FokI FseI FspI HaeII HhaI Hin1I HinP1I HindIII HphI KasI KpnI Ksp632I KspI MaeIII MamI MboII MfeI MluNI MroI MscI MslI MunI NaeI NarI NcoI NdeI NgoMIV NheI NlaIII NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacII SalI SapI SbfI ScaI SexAI SfaNI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tsp45I Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AcsI 380 GAATTT [GA]^AATT[CT] AfaI 130 GTAC GT^AC AflIII 270 ACGCGT A^C[GA][CT]GT AhdI 269 GACGCGTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC ApaI 52 GGGCCC GGGCC^C ApoI 380 GAATTT [GA]^AATT[CT] AspEI 269 GACGCGTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspHI 157 GAGCTC G[AT]GC[AT]^C AvaII 38 GGACC G^G[AT]CC BfaI 181 CTAG C^TAG BpmI 88 CTGGAG CTGGAG(16/14) BsaAI 127 CACGTA [CT]AC^GT[GA] BsgI 109 GTGCAG GTGCAG(16/14) BsiHKAI 157 GAGCTC G[AT]GC[AT]^C BsmAI 318 GAGAC GTCTC(1/5) BsmBI 318 GAGACG CGTCTC(1/5) BsmFI 37 GGGAC GGGAC(10/14) BsmI 300 GAATGC GAATGC(1/-1) BsrDI 364 GCAATG GCAATG(2/0) BstYI 228 GGATCT [GA]^GATC[CT] Cac8I 352 GCCCGC GC[ACGT]^[ACGT]GC Eam1105I 269 GACGCGTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoT14I 341 CCAAGG C^C[AT][AT]GG HgaI 269 GACGC GACGC(5/10) HincII 283 GTTAAC GT[CT]^[GA]AC HindII 283 GTTAAC GT[CT]^[GA]AC HpaI 283 GTTAAC GTT^AAC MaeI 181 CTAG C^TAG MaeII 128 ACGT A^CGT MflI 228 GGATCT [GA]^GATC[CT] MluI 270 ACGCGT A^CGCGT MnlI 208 GAGG CCTC(7/6) PleI 121 GACTC GAGTC(4/5) PpuMI 37 GGGACCC [GA]G^G[AT]CC[CT] RsaI 130 GTAC GT^AC SacI 157 GAGCTC GAGCT^C SfcI 295 CTACAG C^T[GA][CT]AG StyI 341 CCAAGG C^C[AT][AT]GG TaqI 119 TCGA T^CGA TfiI 373 GATTC G^A[AT]TC TspRI 333 CAGTG CA[GC]TG[ACGT][ACGT]^ TthHB8I 119 TCGA T^CGA XhoII 228 GGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AccII 64 CGCG CG^CG 271 CGCG AlwI 210 GGATC GGATC(4/5) 228 GGATC BanII 52 GGGCCC G[GA]GC[CT]^C 157 GAGCTC BmyI 52 GGGCCC G[AGT]GC[ACT]^C 157 GAGCTC BsiYI 42 CCCGGTGGCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 43 CCGGTGGCGGG BslI 42 CCCGGTGGCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 43 CCGGTGGCGGG Bsp1286I 52 GGGCCC G[AGT]GC[ACT]^C 157 GAGCTC BstUI 64 CGCG CG^CG 271 CGCG DdeI 305 CTCAG C^T[ACGT]AG 316 CTGAG DpnII 211 GATC ^GATC 229 GATC DraII 37 GGGACCC [GA]G^G[ACGT]CC[CT] 51 GGGGCCC EcoO109I 37 GGGACCC [GA]G^G[ACGT]CC[CT] 51 GGGGCCC HinfI 121 GACTC G^A[ACGT]TC 373 GATTC MboI 211 GATC ^GATC 229 GATC MspA1I 20 CCGCTG C[AC]G^C[GT]G 104 CCGCTG MvnI 64 CGCG CG^CG 271 CGCG MwoI 10 GCTGGTTCCGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 395 GCTCCCCAGGC NdeII 211 GATC ^GATC 229 GATC Sau3AI 211 GATC ^GATC 229 GATC Tsp509I 171 AATT ^AATT 381 AATT ######################################### # 3 OR MORE CUT ENZYMES ( 24 enzymes) # ######################################### AciI AluI BbvI BcnI BsaJI BssKI BstNI Cfr13I EcoRII FauI Fnu4HI HaeIII HapII HpaII ItaI MseI MspI MvaI NciI NlaIV Sau96I ScrFI Tru9I TseI