Restriction map for
pFN21AE1622
##################################
# NO CUT ENZYMES ( 171 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AgeI Alw44I AlwNI
AocI Aor51HI ApaLI AscI AseI
AsnI Asp700I Asp718I AspI AvaI
AviII AvrII BalI BamHI BanI
BbeI BbrPI BbsI BbvCI BcgI
BciVI BclI BfrI BglI BglII
BlnI BlpI Bpu1102I BpuAI BsaBI
BsaHI BsaI BsaWI BseAI BseRI
BsiEI BsiWI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrFI BsrGI BsrI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstF5I
BstPI BstXI BstZ17I Bsu36I CelII
CfoI Cfr10I ClaI CpoI DraI
DraIII DrdI DsaI EaeI EagI
EarI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FbaI FokI FseI FspI HaeII
HhaI Hin1I HinP1I HindIII HphI
KasI KpnI Ksp632I KspI MaeIII
MamI MboII MfeI MluNI MroI
MscI MslI MunI NaeI NarI
NcoI NdeI NgoMIV NheI NlaIII
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PstI PvuI PvuII RcaI
RsrII SacII SalI SapI SbfI
ScaI SexAI SfaNI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI Tsp45I Tth111I Van91I
XbaI XcmI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AcsI 380 GAATTT [GA]^AATT[CT]
AfaI 130 GTAC GT^AC
AflIII 270 ACGCGT A^C[GA][CT]GT
AhdI 269 GACGCGTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 52 GGGCCC GGGCC^C
ApoI 380 GAATTT [GA]^AATT[CT]
AspEI 269 GACGCGTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 157 GAGCTC G[AT]GC[AT]^C
AvaII 38 GGACC G^G[AT]CC
BfaI 181 CTAG C^TAG
BpmI 88 CTGGAG CTGGAG(16/14)
BsaAI 127 CACGTA [CT]AC^GT[GA]
BsgI 109 GTGCAG GTGCAG(16/14)
BsiHKAI 157 GAGCTC G[AT]GC[AT]^C
BsmAI 318 GAGAC GTCTC(1/5)
BsmBI 318 GAGACG CGTCTC(1/5)
BsmFI 37 GGGAC GGGAC(10/14)
BsmI 300 GAATGC GAATGC(1/-1)
BsrDI 364 GCAATG GCAATG(2/0)
BstYI 228 GGATCT [GA]^GATC[CT]
Cac8I 352 GCCCGC GC[ACGT]^[ACGT]GC
Eam1105I 269 GACGCGTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT14I 341 CCAAGG C^C[AT][AT]GG
HgaI 269 GACGC GACGC(5/10)
HincII 283 GTTAAC GT[CT]^[GA]AC
HindII 283 GTTAAC GT[CT]^[GA]AC
HpaI 283 GTTAAC GTT^AAC
MaeI 181 CTAG C^TAG
MaeII 128 ACGT A^CGT
MflI 228 GGATCT [GA]^GATC[CT]
MluI 270 ACGCGT A^CGCGT
MnlI 208 GAGG CCTC(7/6)
PleI 121 GACTC GAGTC(4/5)
PpuMI 37 GGGACCC [GA]G^G[AT]CC[CT]
RsaI 130 GTAC GT^AC
SacI 157 GAGCTC GAGCT^C
SfcI 295 CTACAG C^T[GA][CT]AG
StyI 341 CCAAGG C^C[AT][AT]GG
TaqI 119 TCGA T^CGA
TfiI 373 GATTC G^A[AT]TC
TspRI 333 CAGTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 119 TCGA T^CGA
XhoII 228 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AccII 64 CGCG CG^CG
271 CGCG
AlwI 210 GGATC GGATC(4/5)
228 GGATC
BanII 52 GGGCCC G[GA]GC[CT]^C
157 GAGCTC
BmyI 52 GGGCCC G[AGT]GC[ACT]^C
157 GAGCTC
BsiYI 42 CCCGGTGGCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
43 CCGGTGGCGGG
BslI 42 CCCGGTGGCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
43 CCGGTGGCGGG
Bsp1286I 52 GGGCCC G[AGT]GC[ACT]^C
157 GAGCTC
BstUI 64 CGCG CG^CG
271 CGCG
DdeI 305 CTCAG C^T[ACGT]AG
316 CTGAG
DpnII 211 GATC ^GATC
229 GATC
DraII 37 GGGACCC [GA]G^G[ACGT]CC[CT]
51 GGGGCCC
EcoO109I 37 GGGACCC [GA]G^G[ACGT]CC[CT]
51 GGGGCCC
HinfI 121 GACTC G^A[ACGT]TC
373 GATTC
MboI 211 GATC ^GATC
229 GATC
MspA1I 20 CCGCTG C[AC]G^C[GT]G
104 CCGCTG
MvnI 64 CGCG CG^CG
271 CGCG
MwoI 10 GCTGGTTCCGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
395 GCTCCCCAGGC
NdeII 211 GATC ^GATC
229 GATC
Sau3AI 211 GATC ^GATC
229 GATC
Tsp509I 171 AATT ^AATT
381 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 24 enzymes) #
#########################################
AciI AluI BbvI BcnI BsaJI
BssKI BstNI Cfr13I EcoRII FauI
Fnu4HI HaeIII HapII HpaII ItaI
MseI MspI MvaI NciI NlaIV
Sau96I ScrFI Tru9I TseI