Restriction map for
pFN21AE1813
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# NO CUT ENZYMES ( 156 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AviII AvrII
BamHI BbeI BbrPI BbvCI BcgI
BciVI BclI BfrI BglII BlnI
BlpI Bpu1102I BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BsiEI BsiHKAI
BsiWI BsmBI BsmI BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrFI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstZ17I
CelII CfoI Cfr10I ClaI CpoI
DraI DraIII DrdI DsaI EagI
Eam1105I EclXI Eco47III Eco52I EcoNI
EcoO65I EcoRI EcoRV EcoT14I EcoT22I
FbaI FseI FspI HaeII HgaI
HhaI Hin1I HinP1I HindIII HpaI
KasI KpnI KspI MaeII MamI
MfeI MluI MroI MunI MvnI
NaeI NarI NcoI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PstI PvuI RcaI RsrII SacI
SacII SapI SbfI ScaI SexAI
SfcI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspI StuI StyI SwaI Tsp45I
Van91I XbaI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AccI 355 GTCGAC GT^[AC][GT]AC
AlwI 678 GATCC GGATC(4/5)
AlwNI 347 CAGACTCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 52 CCTGAGG CC^T[ACGT]AGG
AspI 349 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 727 GGACC G^G[AT]CC
BanI 436 GGTGCC G^G[CT][GA]CC
BbsI 90 GAAGAC GAAGAC(2/6)
BcnI 576 CCGGG CC^[GC]GG
BfaI 499 CTAG C^TAG
BglI 961 GCCAACCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 442 CTGGAG CTGGAG(16/14)
BpuAI 90 GAAGAC GAAGAC(2/6)
BseRI 55 GAGGAG GAGGAG(10/8)
BsgI 565 GTGCAG GTGCAG(16/14)
Bsp1407I 632 TGTACA T^GTACA
BsrDI 957 CATTGC GCAATG(2/0)
BsrGI 632 TGTACA T^GTACA
BstYI 677 AGATCC [GA]^GATC[CT]
Bsu36I 52 CCTGAGG CC^T[ACGT]AGG
DpnII 678 GATC ^GATC
DraII 726 GGGACCT [GA]G^G[ACGT]CC[CT]
Eco81I 52 CCTGAGG CC^T[ACGT]AGG
EcoO109I 726 GGGACCT [GA]G^G[ACGT]CC[CT]
FauI 299 CCCGC CCCGC(4/6)
HapII 576 CCGG C^CGG
HincII 355 GTCGAC GT[CT]^[GA]AC
HindII 355 GTCGAC GT[CT]^[GA]AC
HpaII 576 CCGG C^CGG
HphI 33 GGTGA GGTGA(8/7)
MaeI 499 CTAG C^TAG
MaeIII 379 GTTAC ^GT[ACGT]AC
MboI 678 GATC ^GATC
MflI 677 AGATCC [GA]^GATC[CT]
MslI 205 CATACATATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 888 CAGCTG C[AC]G^C[GT]G
MspI 576 CCGG C^CGG
NciI 576 CCGGG CC^[GC]GG
NdeII 678 GATC ^GATC
PflFI 349 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 726 GGGACCT [GA]G^G[AT]CC[CT]
PvuII 888 CAGCTG CAG^CTG
SalI 355 GTCGAC G^TCGAC
Sau3AI 678 GATC ^GATC
SspBI 632 TGTACA T^GTACA
TfiI 851 GAATC G^A[AT]TC
Tth111I 349 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 245 CCAGTGAAGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 677 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AciI 300 CCGC CCGC(-3/-1)
829 GCGG
AvaI 682 CCCGAG C^[CT]CG[GA]G
719 CCCGAG
BalI 740 TGGCCA TGG^CCA
968 TGGCCA
BanII 7 GGGCTC G[GA]GC[CT]^C
341 GAGCCC
BsmAI 291 GTCTC GTCTC(1/5)
733 GTCTC
BsoBI 682 CCCGAG C^[CT]CG[GA]G
719 CCCGAG
BstF5I 174 CATCC GGATG(2/0)
123 GGATG
Cac8I 751 GCAAGC GC[ACGT]^[ACGT]GC
970 GCCAGC
EaeI 740 TGGCCA [CT]^GGCC[GA]
968 TGGCCA
FokI 174 CATCC GGATG(9/13)
123 GGATG
MluNI 740 TGGCCA TGG^CCA
968 TGGCCA
MscI 740 TGGCCA TGG^CCA
968 TGGCCA
NdeI 209 CATATG CA^TATG
605 CATATG
SfaNI 595 GATGC GCATC(5/9)
748 GATGC
Tsp509I 144 AATT ^AATT
879 AATT
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# 3 OR MORE CUT ENZYMES ( 38 enzymes) #
#########################################
AfaI AluI BbvI BmyI BsaJI
BsiYI BslI BsmFI Bsp1286I BsrI
BssKI BstNI Cfr13I DdeI EarI
EcoRII Fnu4HI HaeIII HinfI ItaI
Ksp632I MboII MnlI MseI MvaI
MwoI NlaIII NlaIV PleI RsaI
Sau96I ScrFI SmlI TaqI Tru9I
TseI TspRI TthHB8I