Restriction map for
pFN21AE6645
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# NO CUT ENZYMES ( 165 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AspI AvaI
AviII AvrII BanI BbeI BbrPI
BbsI BcgI BciVI BcnI BfaI
BfrI BglI BlnI BlpI Bpu1102I
BpuAI BsaAI BsaHI BsaI BsaWI
BseAI BsiEI BsiHKAI BsiWI BsmBI
BsmI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstZ17I Bsu36I
CelII CfoI Cfr10I ClaI CpoI
DraI DrdI DsaI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoT14I FseI FspI HaeII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HpaI HpaII HphI KasI
KpnI KspI MaeI MaeII MfeI
MluI MroI MspA1I MspI MunI
MvnI MwoI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NotI
NruI NspI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PstI PvuI
PvuII RcaI RsrII SacI SacII
SalI SapI SbfI SexAI SfaNI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI StuI StyI SwaI Tth111I
XbaI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AciI 41 GCGG CCGC(-3/-1)
AfaI 510 GTAC GT^AC
AluI 478 AGCT AG^CT
AlwNI 259 CAGGTCCTG CAG[ACGT][ACGT][ACGT]^CTG
AvaII 261 GGTCC G^G[AT]CC
BamHI 659 GGATCC G^GATCC
BbvCI 636 CCTCAGC CCTCAGC(-5/-2)
BclI 176 TGATCA T^GATCA
BglII 737 AGATCT A^GATCT
BsaBI 229 GATCATTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 32 GAGGAG GAGGAG(10/8)
BsgI 333 GTGCAG GTGCAG(16/14)
BsmAI 524 GAGAC GTCTC(1/5)
BsmFI 677 GGGAC GGGAC(10/14)
BspMI 627 GCAGGT ACCTGC(4/8)
BsrBI 39 GAGCGG CCGCTC(-3/-3)
BsrDI 840 CATTGC GCAATG(2/0)
BstF5I 278 GGATG GGATG(2/0)
Cac8I 41 GCGGGC GC[ACGT]^[ACGT]GC
DraII 260 AGGTCCT [GA]G^G[ACGT]CC[CT]
DraIII 327 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoNI 142 CCTGAAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 260 AGGTCCT [GA]G^G[ACGT]CC[CT]
EcoRI 582 GAATTC G^AATTC
EcoRV 686 GATATC GAT^ATC
EcoT22I 247 ATGCAT ATGCA^T
FauI 41 GCGGG CCCGC(4/6)
FbaI 176 TGATCA T^GATCA
FokI 278 GGATG GGATG(9/13)
HindIII 477 AAGCTT A^AGCTT
MamI 229 GATCATTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MseI 320 TTAA T^TAA
MslI 451 CACACCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NsiI 247 ATGCAT ATGCA^T
PflMI 258 CCAGGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 260 AGGTCCT [GA]G^G[AT]CC[CT]
RsaI 510 GTAC GT^AC
ScaI 509 AGTACT AGT^ACT
SfcI 602 CTGTAG C^T[GA][CT]AG
SspI 764 AATATT AAT^ATT
Tru9I 320 TTAA T^TAA
Van91I 258 CCAGGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 798 CCATGAAATGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 22 enzymes) #
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AcsI 292 GAATTT [GA]^AATT[CT]
582 GAATTC
ApoI 292 GAATTT [GA]^AATT[CT]
582 GAATTC
BalI 444 TGGCCA TGG^CCA
744 TGGCCA
BanII 43 GGGCTC G[GA]GC[CT]^C
118 GGGCTC
BbvI 348 GCAGC GCAGC(8/12)
62 GCTGC
BmyI 43 GGGCTC G[AGT]GC[ACT]^C
118 GGGCTC
BsaJI 257 CCCAGG C^C[ACGT][ACGT]GG
483 CCCTGG
Bsp1286I 43 GGGCTC G[AGT]GC[ACT]^C
118 GGGCTC
Cfr13I 155 GGGCC G^G[ACGT]CC
261 GGTCC
DdeI 5 CTAAG C^T[ACGT]AG
637 CTCAG
EaeI 444 TGGCCA [CT]^GGCC[GA]
744 TGGCCA
EarI 48 CTCTTC CTCTTC(1/4)
708 CTCTTC
Fnu4HI 62 GCTGC GC^[ACGT]GC
348 GCAGC
ItaI 62 GCTGC GC^[ACGT]GC
348 GCAGC
Ksp632I 48 CTCTTC CTCTTC(1/4)
708 CTCTTC
MaeIII 212 GTGAC ^GT[ACGT]AC
385 GTCAC
MluNI 444 TGGCCA TGG^CCA
744 TGGCCA
MscI 444 TGGCCA TGG^CCA
744 TGGCCA
PleI 428 GAGTC GAGTC(4/5)
560 GAGTC
Sau96I 155 GGGCC G^G[ACGT]CC
261 GGTCC
TseI 62 GCTGC G^C[AT]GC
348 GCAGC
Tsp45I 212 GTGAC ^GT[GC]AC
385 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 28 enzymes) #
#########################################
AlwI BpmI BsiYI BslI BsrI
BssKI BstNI BstYI DpnII EcoRII
HaeIII HinfI MboI MboII MflI
MnlI MvaI NdeII NlaIII NlaIV
Sau3AI ScrFI TaqI TfiI Tsp509I
TspRI TthHB8I XhoII