Restriction map for
pFN21AE5328
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# NO CUT ENZYMES ( 145 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AflII AflIII AgeI
Alw44I Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspI AviII AvrII BalI BamHI
BbrPI BbvCI BcgI BciVI BclI
BfaI BfrI BglI BglII BlnI
BlpI BpmI Bpu1102I BsaAI BsaWI
BseAI BsiWI BsmBI BsmI Bsp1407I
BspDI BspEI BspLU11I BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstUI BstXI
BstYI BstZ17I CelII Cfr10I ClaI
CpoI DraIII DrdI EagI EarI
EclXI Eco47III Eco52I EcoRI EcoRV
FauI FbaI FseI FspI HgaI
HincII HindII HindIII HpaI KpnI
Ksp632I KspI MaeI MaeII MflI
MluI MluNI MroI MscI MvnI
NaeI NcoI NdeI NgoMIV NheI
NotI NruI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PstI PvuI PvuII RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfcI SfiI SfuI SgrAI
SmlI SnaBI SpeI SphI Sse8387I
SspBI StuI SwaI Tth111I Van91I
XbaI XcmI XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 55 enzymes) #
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AcyI 43 GGCGCC G[GA]^CG[CT]C
AfaI 116 GTAC GT^AC
AhdI 19 GACCGGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 413 CAGAGCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 355 CCTGAGG CC^T[ACGT]AGG
AspEI 19 GACCGGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 84 CCCGGG C^[CT]CG[GA]G
BanI 43 GGCGCC G^G[CT][GA]CC
BanII 694 GAGCCC G[GA]GC[CT]^C
BbeI 43 GGCGCC GGCGC^C
BbsI 268 GTCTTC GAAGAC(2/6)
BpuAI 268 GTCTTC GAAGAC(2/6)
BsaBI 602 GATTCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 43 GGCGCC G[GA]^CG[CT]C
BsaI 536 GAGACC GGTCTC(1/5)
BseRI 626 CTCCTC GAGGAG(10/8)
BsgI 53 CTGCAC GTGCAG(16/14)
BsiEI 18 CGACCG CG[GA][CT]^CG
BsmFI 164 GGGAC GGGAC(10/14)
BsoBI 84 CCCGGG C^[CT]CG[GA]G
BspHI 402 TCATGA T^CATGA
BsrI 428 CCAGT ACTGG(1/-1)
BstEII 423 GGTTACC G^GT[ACGT]ACC
BstPI 423 GGTTACC G^GT[ACGT]ACC
Bsu36I 355 CCTGAGG CC^T[ACGT]AGG
DraI 495 TTTAAA TTT^AAA
DsaI 39 CCACGG C^C[GA][CT]GG
EaeI 75 CGGCCA [CT]^GGCC[GA]
Eam1105I 19 GACCGGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 355 CCTGAGG CC^T[ACGT]AGG
EcoNI 351 CCTCCCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 423 GGTTACC G^GT[ACGT]ACC
EcoT14I 47 CCAAGG C^C[AT][AT]GG
EcoT22I 725 ATGCAT ATGCA^T
Hin1I 43 GGCGCC G[GA]^CG[CT]C
HphI 550 TCACC GGTGA(8/7)
KasI 43 GGCGCC G^GCGCC
MamI 602 GATTCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 291 CAATTG C^AATTG
MslI 718 CATCTCTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 243 CCGCTG C[AC]G^C[GT]G
MunI 291 CAATTG C^AATTG
NarI 43 GGCGCC GG^CGCC
NsiI 725 ATGCAT ATGCA^T
PpuMI 280 GGGTCCT [GA]G^G[AT]CC[CT]
RcaI 402 TCATGA T^CATGA
RsaI 116 GTAC GT^AC
SfaNI 490 GATGC GCATC(5/9)
SfoI 43 GGCGCC GGC^GCC
SmaI 84 CCCGGG CCC^GGG
SspI 664 AATATT AAT^ATT
StyI 47 CCAAGG C^C[AT][AT]GG
Tsp45I 674 GTGAC ^GT[GC]AC
XmaCI 84 CCCGGG C^CCGGG
XmaI 84 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 13 enzymes) #
###################################
AciI 243 CCGC CCGC(-3/-1)
74 GCGG
AlwI 406 GATCC GGATC(4/5)
456 GATCC
BbvI 186 GCAGC GCAGC(8/12)
52 GCTGC
BsmAI 523 GAGAC GTCTC(1/5)
536 GAGAC
DraII 280 GGGTCCT [GA]G^G[ACGT]CC[CT]
558 AGGGCCT
EcoO109I 280 GGGTCCT [GA]G^G[ACGT]CC[CT]
558 AGGGCCT
HaeII 35 AGCGCC [GA]GCGC^[CT]
43 GGCGCC
MboII 780 GAAGA GAAGA(8/7)
269 TCTTC
NlaIV 43 GGCGCC GG[ACGT]^[ACGT]CC
280 GGGTCC
PleI 148 GACTC GAGTC(4/5)
202 GACTC
TaqI 488 TCGA T^CGA
588 TCGA
TseI 52 GCTGC G^C[AT]GC
186 GCAGC
TthHB8I 488 TCGA T^CGA
588 TCGA
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# 3 OR MORE CUT ENZYMES ( 45 enzymes) #
#########################################
AluI AspHI AvaII BcnI BmyI
BsaJI BsiHKAI BsiYI BslI Bsp1286I
BssKI BstF5I BstNI Cac8I CfoI
Cfr13I DdeI DpnII EcoRII Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII ItaI MaeIII MboI
MnlI MseI MspI MvaI MwoI
NciI NdeII NlaIII Sau3AI Sau96I
ScrFI TfiI Tru9I Tsp509I TspRI