Restriction map for
pFN21AE6505
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# NO CUT ENZYMES ( 168 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AcsI AcyI AfaI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp718I AspEI
AspHI AspI AviII AvrII BbeI
BbrPI BbsI BcgI BciVI BcnI
BfaI BfrI BglI BglII BlnI
BlpI Bpu1102I BpuAI BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BseRI
BsgI BsiEI BsiHKAI BsiWI BsmAI
BsmBI BsmFI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstUI BstXI BstZ17I Bsu36I
Cac8I CelII Cfr10I ClaI CpoI
DraI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoRI EcoRV FauI FseI FspI
HaeII HapII HgaI Hin1I HincII
HindII HindIII HpaI HpaII KasI
KpnI KspI MaeI MaeII MamI
MfeI MluI MroI MspI MunI
MvnI NaeI NarI NciI NdeI
NgoMIV NheI NotI NruI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI Tth111I Van91I XbaI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 46 enzymes) #
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Asp700I 367 GAAGGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 473 CCCGAG C^[CT]CG[GA]G
AvaII 258 GGACC G^G[AT]CC
BalI 233 TGGCCA TGG^CCA
BamHI 100 GGATCC G^GATCC
BanI 281 GGTGCC G^G[CT][GA]CC
BanII 571 GGGCTC G[GA]GC[CT]^C
BclI 15 TGATCA T^GATCA
BpmI 463 CTGGAG CTGGAG(16/14)
BsiYI 90 CCGACCTGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 90 CCGACCTGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsoBI 473 CCCGAG C^[CT]CG[GA]G
BspMI 260 ACCTGC ACCTGC(4/8)
BstEII 85 GGTGACC G^GT[ACGT]ACC
BstPI 85 GGTGACC G^GT[ACGT]ACC
CfoI 315 GCGC GCG^C
Cfr13I 258 GGACC G^G[ACGT]CC
DraII 257 AGGACCT [GA]G^G[ACGT]CC[CT]
EaeI 233 TGGCCA [CT]^GGCC[GA]
EarI 508 CTCTTC CTCTTC(1/4)
EcoO109I 257 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoO65I 85 GGTGACC G^GT[ACGT]ACC
EcoT14I 236 CCATGG C^C[AT][AT]GG
EcoT22I 9 ATGCAT ATGCA^T
FbaI 15 TGATCA T^GATCA
HhaI 315 GCGC GCG^C
HinP1I 315 GCGC G^CGC
Ksp632I 508 CTCTTC CTCTTC(1/4)
MaeIII 86 GTGAC ^GT[ACGT]AC
MluNI 233 TGGCCA TGG^CCA
MscI 233 TGGCCA TGG^CCA
MseI 418 TTAA T^TAA
MslI 397 CATTCCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI 236 CCATGG C^CATGG
NsiI 9 ATGCAT ATGCA^T
PleI 299 GACTC GAGTC(4/5)
PpuMI 257 AGGACCT [GA]G^G[AT]CC[CT]
PstI 262 CTGCAG CTGCA^G
Sau96I 258 GGACC G^G[ACGT]CC
StyI 236 CCATGG C^C[AT][AT]GG
TfiI 114 GATTC G^A[AT]TC
Tru9I 418 TTAA T^TAA
Tsp45I 86 GTGAC ^GT[GC]AC
Tsp509I 420 AATT ^AATT
XcmI 434 CCAACTTTACCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 367 GAAGGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 26 enzymes) #
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AlwNI 256 CAGGACCTG CAG[ACGT][ACGT][ACGT]^CTG
518 CAGATGCTG
BbvCI 539 CCTCAGC CCTCAGC(-5/-2)
34 GCTGAGG
BmyI 282 GTGCCC G[AGT]GC[ACT]^C
571 GGGCTC
BsmI 7 GAATGC GAATGC(1/-1)
593 GCATTC
Bsp1286I 282 GTGCCC G[AGT]GC[ACT]^C
571 GGGCTC
BssKI 94 CCTGG ^CC[ACGT]GG
130 CCTGG
BstF5I 228 GGATG GGATG(2/0)
468 GGATG
BstNI 94 CCTGG CC^[AT]GG
130 CCTGG
BstYI 100 GGATCC [GA]^GATC[CT]
146 AGATCC
DsaI 236 CCATGG C^C[GA][CT]GG
443 CCGTGG
EcoRII 94 CCTGG ^CC[AT]GG
130 CCTGG
FokI 228 GGATG GGATG(9/13)
468 GGATG
HaeIII 234 GGCC GG^CC
240 GGCC
HinfI 114 GATTC G^A[ACGT]TC
299 GACTC
HphI 85 GGTGA GGTGA(8/7)
427 GGTGA
MflI 100 GGATCC [GA]^GATC[CT]
146 AGATCC
MspA1I 191 CAGCTG C[AC]G^C[GT]G
248 CAGCTG
MvaI 94 CCTGG CC^[AT]GG
130 CCTGG
NlaIII 139 CATG CATG^
237 CATG
PvuII 191 CAGCTG CAG^CTG
248 CAGCTG
ScrFI 94 CCTGG CC^[ACGT]GG
130 CCTGG
SfcI 53 CTGTAG C^T[GA][CT]AG
262 CTGCAG
TaqI 112 TCGA T^CGA
306 TCGA
TspRI 377 CAGTG CA[GC]TG[ACGT][ACGT]^
461 CACTG
TthHB8I 112 TCGA T^CGA
306 TCGA
XhoII 100 GGATCC [GA]^GATC[CT]
146 AGATCC
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# 3 OR MORE CUT ENZYMES ( 18 enzymes) #
#########################################
AluI AlwI BbvI BsaJI BsrI
DdeI DpnII Fnu4HI ItaI MboI
MboII MnlI MwoI NdeII NlaIV
Sau3AI SfaNI TseI