Restriction map for
pFN21AE3960
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# NO CUT ENZYMES ( 114 enzymes) #
##################################
AatII AccII AflII AgeI AhdI
Alw44I Aor51HI ApaLI AscI AseI
AsnI Asp700I AspEI AspI AviII
BamHI BbeI BbvCI BcgI BciVI
BclI BfrI BglI BglII BlpI
Bpu1102I BsaI BsiEI BsiWI BsmBI
Bsp1407I BspDI BspHI BspLU11I BsrGI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstUI BstZ17I CelII ClaI
CpoI DraI EagI Eam1105I EclXI
Eco47III Eco52I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HaeII HincII HindII HindIII HpaI
KasI KspI MfeI MluI MunI
MvnI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmeI
PpuMI PshAI PshBI PvuI RcaI
RsrII SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SnaBI SphI Sse8387I SspBI
StuI SwaI TaqI TfiI Tth111I
TthHB8I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
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Acc65I 1544 GGTACC G^GTACC
AccI 1664 GTCTAC GT^[AC][GT]AC
AccIII 830 TCCGGA T^CCGGA
AclI 1258 AACGTT AA^CGTT
AcyI 1369 GGCGTC G[GA]^CG[CT]C
AfaI 1545 GTAC GT^AC
AflIII 151 ACGTGT A^C[GA][CT]GT
AocI 1126 CCTCAGG CC^T[ACGT]AGG
ApaI 908 GGGCCC GGGCC^C
Asp718I 1544 GGTACC G^GTACC
AvaI 1181 CCCGGG C^[CT]CG[GA]G
AvrII 1548 CCTAGG C^CTAGG
BalI 1557 TGGCCA TGG^CCA
BbsI 898 GAAGAC GAAGAC(2/6)
BlnI 1548 CCTAGG C^CTAGG
BpuAI 898 GAAGAC GAAGAC(2/6)
BsaHI 1369 GGCGTC G[GA]^CG[CT]C
BsaWI 830 TCCGGA [AT]^CCGG[AT]
BseAI 830 TCCGGA T^CCGGA
BsmI 1627 GAATGC GAATGC(1/-1)
BsoBI 1181 CCCGGG C^[CT]CG[GA]G
BspEI 830 TCCGGA T^CCGGA
BsrBI 1508 CCGCTC CCGCTC(-3/-3)
BsrDI 683 GCAATG GCAATG(2/0)
BsrFI 620 ACCGGC [GA]^CCGG[CT]
BstAPI 1478 GCACCTTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 1126 CCTCAGG CC^T[ACGT]AGG
Cfr10I 620 ACCGGC [GA]^CCGG[CT]
DraIII 147 CACCACGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1294 GACAGCCCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 432 GAAGAG CTCTTC(1/4)
Eco81I 1126 CCTCAGG CC^T[ACGT]AGG
FauI 1493 GCGGG CCCGC(4/6)
HgaI 1370 GCGTC GACGC(5/10)
Hin1I 1369 GGCGTC G[GA]^CG[CT]C
KpnI 1544 GGTACC GGTAC^C
Ksp632I 432 GAAGAG CTCTTC(1/4)
MluNI 1557 TGGCCA TGG^CCA
MroI 830 TCCGGA T^CCGGA
MscI 1557 TGGCCA TGG^CCA
Psp1406I 1258 AACGTT AA^CGTT
RsaI 1545 GTAC GT^AC
SacI 718 GAGCTC GAGCT^C
SmaI 1181 CCCGGG CCC^GGG
SmlI 241 CTCAAG C^T[CT][GA]AG
SpeI 46 ACTAGT A^CTAGT
SspI 228 AATATT AAT^ATT
XmaCI 1181 CCCGGG C^CCGGG
XmaI 1181 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AlwI 774 GATCC GGATC(4/5)
1026 GATCC
AlwNI 391 CAGGGCCTG CAG[ACGT][ACGT][ACGT]^CTG
955 CAGTTACTG
AspHI 718 GAGCTC G[AT]GC[AT]^C
994 GAGCAC
BanI 1528 GGCACC G^G[CT][GA]CC
1544 GGTACC
BbrPI 150 CACGTG CAC^GTG
742 CACGTG
BcnI 1181 CCCGG CC^[GC]GG
1182 CCGGG
BfaI 47 CTAG C^TAG
1549 CTAG
BsaBI 267 GATCGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1026 GATCCTCATC
BsiHKAI 718 GAGCTC G[AT]GC[AT]^C
994 GAGCAC
BsmAI 200 GAGAC GTCTC(1/5)
1253 GAGAC
BspMI 305 ACCTGC ACCTGC(4/8)
445 ACCTGC
BstYI 773 AGATCC [GA]^GATC[CT]
1025 AGATCC
CfoI 1517 GCGC GCG^C
1652 GCGC
DraII 392 AGGGCCT [GA]G^G[ACGT]CC[CT]
749 AGGCCCT
EaeI 347 TGGCCG [CT]^GGCC[GA]
1557 TGGCCA
EcoO109I 392 AGGGCCT [GA]G^G[ACGT]CC[CT]
749 AGGCCCT
HhaI 1517 GCGC GCG^C
1652 GCGC
HinP1I 1517 GCGC G^CGC
1652 GCGC
MaeI 47 CTAG C^TAG
1549 CTAG
MamI 267 GATCGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1026 GATCCTCATC
MflI 773 AGATCC [GA]^GATC[CT]
1025 AGATCC
NciI 1181 CCCGG CC^[GC]GG
1182 CCGGG
NspI 968 ACATGC [GA]CATG^[CT]
1404 GCATGT
PflMI 390 CCAGGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
510 CCACACCATGG
PmaCI 150 CACGTG CAC^GTG
742 CACGTG
PmlI 150 CACGTG CAC^GTG
742 CACGTG
Van91I 390 CCAGGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
510 CCACACCATGG
XcmI 335 CCATAGACCACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1037 CCAAGAGGGTGGTGG
XhoII 773 AGATCC [GA]^GATC[CT]
1025 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 66 enzymes) #
#########################################
AciI AcsI AluI ApoI AvaII
BanII BbvI BmyI BpmI BsaAI
BsaJI BseRI BsgI BsiYI BslI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI BstXI Cac8I Cfr13I DdeI
DpnII DsaI EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HinfI HpaII
HphI ItaI MaeII MaeIII MboI
MboII MnlI MseI MslI MspA1I
MspI MvaI MwoI NcoI NdeII
NlaIII NlaIV PleI PstI PvuII
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI Tru9I TseI Tsp45I Tsp509I
TspRI