Restriction map for
pFN21AE1707
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# NO CUT ENZYMES ( 163 enzymes) #
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AatII Acc65I AccI AccII AclI
AcsI AfaI AflII AgeI AhdI
Alw44I AocI ApaI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvaI AviII AvrII
BalI BamHI BanI BanII BbeI
BbrPI BbsI BbvCI BcgI BciVI
BclI BcnI BfaI BfrI BglI
BglII BlnI BlpI Bpu1102I BpuAI
BsaAI BsaBI BsaI BseRI BsiWI
BsiYI BslI BsmBI BsmI BsoBI
Bsp1407I BspDI BspMI BsrBI BsrDI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstZ17I Bsu36I Cac8I CelII ClaI
CpoI DdeI DraI DraII DraIII
DrdI DsaI Eam1105I Eco81I EcoNI
EcoO109I EcoO65I EcoRI EcoRV FbaI
FseI FspI HincII HindII HindIII
HpaI KasI KpnI KspI MaeI
MaeII MamI MfeI MluI MluNI
MscI MspA1I MunI MvnI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NlaIV NruI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI PvuII RsaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
Tsp45I Tth111I Van91I XbaI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 57 enzymes) #
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AccIII 150 TCCGGA T^CCGGA
AcyI 31 GACGCC G[GA]^CG[CT]C
AflIII 188 ACATGT A^C[GA][CT]GT
AlwNI 429 CAGAATCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 144 AGCGCT AGC^GCT
AspHI 61 GAGCAC G[AT]GC[AT]^C
AvaII 153 GGACC G^G[AT]CC
BmyI 61 GAGCAC G[AGT]GC[ACT]^C
BpmI 46 CTGGAG CTGGAG(16/14)
BsaHI 31 GACGCC G[GA]^CG[CT]C
BsaJI 278 CCTTGG C^C[ACGT][ACGT]GG
BsaWI 150 TCCGGA [AT]^CCGG[AT]
BseAI 150 TCCGGA T^CCGGA
BsiEI 71 CGGCCG CG[GA][CT]^CG
BsiHKAI 61 GAGCAC G[AT]GC[AT]^C
Bsp1286I 61 GAGCAC G[AGT]GC[ACT]^C
BspEI 150 TCCGGA T^CCGGA
BspHI 311 TCATGA T^CATGA
BspLU11I 188 ACATGT A^CATGT
BsrFI 110 ACCGGC [GA]^CCGG[CT]
BssKI 291 CCAGG ^CC[ACGT]GG
BstNI 291 CCAGG CC^[AT]GG
BstYI 20 AGATCC [GA]^GATC[CT]
Cfr10I 110 ACCGGC [GA]^CCGG[CT]
Cfr13I 153 GGACC G^G[ACGT]CC
EaeI 71 CGGCCG [CT]^GGCC[GA]
EagI 71 CGGCCG C^GGCCG
EarI 569 CTCTTC CTCTTC(1/4)
EclXI 71 CGGCCG C^GGCCG
Eco47III 144 AGCGCT AGC^GCT
Eco52I 71 CGGCCG C^GGCCG
EcoRII 291 CCAGG ^CC[AT]GG
EcoT14I 278 CCTTGG C^C[AT][AT]GG
EcoT22I 170 ATGCAT ATGCA^T
HaeIII 72 GGCC GG^CC
Hin1I 31 GACGCC G[GA]^CG[CT]C
HphI 390 TCACC GGTGA(8/7)
Ksp632I 569 CTCTTC CTCTTC(1/4)
MaeIII 336 GTTAC ^GT[ACGT]AC
MflI 20 AGATCC [GA]^GATC[CT]
MroI 150 TCCGGA T^CCGGA
MseI 343 TTAA T^TAA
MslI 289 CACCAGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvaI 291 CCAGG CC^[AT]GG
NotI 70 GCGGCCGC GC^GGCCGC
NsiI 170 ATGCAT ATGCA^T
NspI 188 ACATGT [GA]CATG^[CT]
PstI 404 CTGCAG CTGCA^G
RcaI 311 TCATGA T^CATGA
Sau96I 153 GGACC G^G[ACGT]CC
ScrFI 291 CCAGG CC^[ACGT]GG
SfcI 404 CTGCAG C^T[GA][CT]AG
SgrAI 109 CACCGGCG C[GA]^CCGG[CT]G
StyI 278 CCTTGG C^C[AT][AT]GG
Tru9I 343 TTAA T^TAA
XcmI 35 CCAAGACGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 20 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 21 enzymes) #
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AlwI 21 GATCC GGATC(4/5)
90 GGATC
BsgI 6 CTGCAC GTGCAG(16/14)
106 CTGCAC
BsmFI 139 GGGAC GGGAC(10/14)
219 GTCCC
CfoI 103 GCGC GCG^C
145 GCGC
DpnII 21 GATC ^GATC
91 GATC
HaeII 102 GGCGCT [GA]GCGC^[CT]
144 AGCGCT
HapII 111 CCGG C^CGG
151 CCGG
HgaI 31 GACGC GACGC(5/10)
39 GACGC
HhaI 103 GCGC GCG^C
145 GCGC
HinP1I 103 GCGC G^CGC
145 GCGC
HpaII 111 CCGG C^CGG
151 CCGG
MboI 21 GATC ^GATC
91 GATC
MboII 18 GAAGA GAAGA(8/7)
570 TCTTC
MspI 111 CCGG C^CGG
151 CCGG
NdeII 21 GATC ^GATC
91 GATC
NlaIII 189 CATG CATG^
312 CATG
Sau3AI 21 GATC ^GATC
91 GATC
TaqI 14 TCGA T^CGA
540 TCGA
TfiI 431 GAATC G^A[AT]TC
495 GATTC
TspRI 561 CAGTG CA[GC]TG[ACGT][ACGT]^
600 CACTG
TthHB8I 14 TCGA T^CGA
540 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 17 enzymes) #
#########################################
AciI AluI BbvI BsmAI BsrI
BstF5I FauI Fnu4HI FokI HinfI
ItaI MnlI MwoI PleI SfaNI
TseI Tsp509I