Restriction map for
pFN21AB6255
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# NO CUT ENZYMES ( 137 enzymes) #
##################################
AatII AccII AccIII AclI AcyI
AgeI AhdI Alw44I AlwI AocI
Aor51HI ApaLI AscI Asp700I AspEI
AvaI AviII AvrII BalI BamHI
BbeI BbrPI BbsI BcgI BclI
BcnI BglI BlnI BlpI BpmI
Bpu1102I BpuAI BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BsgI BsiEI
BsiWI BsmI BsoBI Bsp1407I BspDI
BspEI BsrBI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstUI BstXI
BstZ17I Bsu36I Cac8I CelII CfoI
ClaI CpoI DraI DraIII DrdI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoRV EcoT22I
FbaI FseI FspI HaeII HhaI
Hin1I HinP1I HindIII HpaI KasI
KspI MamI MluI MluNI MroI
MscI MvnI NaeI NarI NciI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI Psp1406I PstI PvuI RsrII
SacI SacII SbfI SexAI SfcI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
StuI SwaI Van91I XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
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Acc65I 32 GGTACC G^GTACC
AlwNI 1429 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 1205 GGGCCC GGGCC^C
Asp718I 32 GGTACC G^GTACC
AspHI 1883 GTGCTC G[AT]GC[AT]^C
AspI 707 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbvCI 1528 GCTGAGG CCTCAGC(-5/-2)
BbvI 1033 GCAGC GCAGC(8/12)
BciVI 1837 GTATCC GTATCC(6/5)
BglII 1535 AGATCT A^GATCT
BsiHKAI 1883 GTGCTC G[AT]GC[AT]^C
BsmBI 1803 GAGACG CGTCTC(1/5)
BspMI 1154 ACCTGC ACCTGC(4/8)
BsrDI 2018 GCAATG GCAATG(2/0)
BsrFI 1175 GCCGGT [GA]^CCGG[CT]
BstEII 279 GGTCACC G^GT[ACGT]ACC
BstPI 279 GGTCACC G^GT[ACGT]ACC
BstYI 1535 AGATCT [GA]^GATC[CT]
Cfr10I 1175 GCCGGT [GA]^CCGG[CT]
DsaI 205 CCATGG C^C[GA][CT]GG
EcoO65I 279 GGTCACC G^GT[ACGT]ACC
EcoRI 1290 GAATTC G^AATTC
Fnu4HI 1033 GCAGC GC^[ACGT]GC
HapII 1176 CCGG C^CGG
HgaI 1977 GCGTC GACGC(5/10)
HpaII 1176 CCGG C^CGG
ItaI 1033 GCAGC GC^[ACGT]GC
KpnI 32 GGTACC GGTAC^C
MflI 1535 AGATCT [GA]^GATC[CT]
MslI 1383 CATCATCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 1526 CAGCTG C[AC]G^C[GT]G
MspI 1176 CCGG C^CGG
NcoI 205 CCATGG C^CATGG
PflFI 707 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PvuII 1526 CAGCTG CAG^CTG
SalI 713 GTCGAC G^TCGAC
SapI 1497 GAAGAGC GCTCTTC(1/4)
ScaI 381 AGTACT AGT^ACT
TseI 1033 GCAGC G^C[AT]GC
Tth111I 707 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 1051 TCTAGA T^CTAGA
XcmI 36 CCAAAAGAAGATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 1535 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AccI 438 GTCTAC GT^[AC][GT]AC
713 GTCGAC
AciI 1116 CCGC CCGC(-3/-1)
993 GCGG
AflII 1750 CTTAAG C^TTAAG
2059 CTTAAG
AflIII 1088 ACATGT A^C[GA][CT]GT
1139 ACATGT
AvaII 388 GGACC G^G[AT]CC
1232 GGACC
BanII 1047 GGGCTC G[GA]GC[CT]^C
1205 GGGCCC
BfrI 1750 CTTAAG C^TTAAG
2059 CTTAAG
BspHI 1388 TCATGA T^CATGA
1637 TCATGA
BspLU11I 1088 ACATGT A^CATGT
1139 ACATGT
BssKI 1044 CCAGG ^CC[ACGT]GG
1712 CCAGG
BstNI 1044 CCAGG CC^[AT]GG
1712 CCAGG
DraII 886 GGGGCCT [GA]G^G[ACGT]CC[CT]
1204 GGGGCCC
EcoO109I 886 GGGGCCT [GA]G^G[ACGT]CC[CT]
1204 GGGGCCC
EcoRII 1044 CCAGG ^CC[AT]GG
1712 CCAGG
EcoT14I 205 CCATGG C^C[AT][AT]GG
1896 CCAAGG
FauI 1115 CCCGC CCCGC(4/6)
993 GCGGG
MaeII 155 ACGT A^CGT
1239 ACGT
MfeI 264 CAATTG C^AATTG
338 CAATTG
MunI 264 CAATTG C^AATTG
338 CAATTG
MvaI 1044 CCAGG CC^[AT]GG
1712 CCAGG
NspI 1088 ACATGT [GA]CATG^[CT]
1139 ACATGT
RcaI 1388 TCATGA T^CATGA
1637 TCATGA
ScrFI 1044 CCAGG CC^[ACGT]GG
1712 CCAGG
SspI 1765 AATATT AAT^ATT
2028 AATATT
StyI 205 CCATGG C^C[AT][AT]GG
1896 CCAAGG
#########################################
# 3 OR MORE CUT ENZYMES ( 53 enzymes) #
#########################################
AcsI AfaI AluI ApoI AseI
AsnI BanI BfaI BmyI BsaJI
BseRI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrI BstF5I Cfr13I DdeI
DpnII EarI FokI HaeIII HincII
HindII HinfI HphI Ksp632I MaeI
MaeIII MboI MboII MnlI MseI
MwoI NdeII NlaIII NlaIV PleI
PshBI RsaI Sau3AI Sau96I SfaNI
SmlI TaqI TfiI Tru9I Tsp45I
Tsp509I TspRI TthHB8I