Restriction map for
pFN21AE6282
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# NO CUT ENZYMES ( 159 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AspI AviII BalI
BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BfrI
BglII BlpI BmyI BpmI Bpu1102I
BpuAI BsaBI BsaHI BsaWI BseAI
BseRI BsiEI BsiHKAI BsiWI BsmBI
Bsp1286I Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrFI BsrGI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstUI BstXI BstYI BstZ17I
CelII Cfr10I ClaI CpoI DraI
DrdI DsaI EaeI EagI Eam1105I
EclXI Eco47III Eco52I EcoNI EcoO65I
EcoRV EcoT22I FauI FbaI FseI
FspI Hin1I HincII HindII HindIII
HpaI HphI KasI KpnI KspI
MamI MflI MluI MluNI MroI
MscI MvnI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PleI PmeI
PshAI PshBI Psp1406I PstI PvuI
PvuII RcaI RsrII SacI SacII
SalI SapI SbfI SexAI SfcI
SfiI SfoI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI Tth111I Van91I XbaI
XcmI XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AcsI 184 GAATTC [GA]^AATT[CT]
AluI 731 AGCT AG^CT
AlwI 174 GATCC GGATC(4/5)
AlwNI 469 CAGGCACTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 178 CCTGAGG CC^T[ACGT]AGG
ApoI 184 GAATTC [GA]^AATT[CT]
AvaI 8 CCCGGG C^[CT]CG[GA]G
AvrII 66 CCTAGG C^CTAGG
BbrPI 664 CACGTG CAC^GTG
BglI 31 GCCACAGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 66 CCTAGG C^CTAGG
BsaAI 664 CACGTG [CT]AC^GT[GA]
BsaI 292 GAGACC GGTCTC(1/5)
BsgI 607 GTGCAG GTGCAG(16/14)
BsoBI 8 CCCGGG C^[CT]CG[GA]G
Bsu36I 178 CCTGAGG CC^T[ACGT]AGG
DdeI 179 CTGAG C^T[ACGT]AG
DpnII 174 GATC ^GATC
DraIII 664 CACGTGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 178 CCTGAGG CC^T[ACGT]AGG
EcoRI 184 GAATTC G^AATTC
EcoT14I 66 CCTAGG C^C[AT][AT]GG
HaeII 485 AGCGCC [GA]GCGC^[CT]
HaeIII 39 GGCC GG^CC
HgaI 621 GCGTC GACGC(5/10)
HinfI 582 GAATC G^A[ACGT]TC
MaeII 665 ACGT A^CGT
MboI 174 GATC ^GATC
MseI 338 TTAA T^TAA
MslI 519 CATTGCTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 35 CAGCGG C[AC]G^C[GT]G
NdeII 174 GATC ^GATC
PmaCI 664 CACGTG CAC^GTG
PmlI 664 CACGTG CAC^GTG
Sau3AI 174 GATC ^GATC
ScaI 312 AGTACT AGT^ACT
SmaI 8 CCCGGG CCC^GGG
SmlI 749 CTCAAG C^T[CT][GA]AG
StyI 66 CCTAGG C^C[AT][AT]GG
TaqI 624 TCGA T^CGA
TfiI 582 GAATC G^A[AT]TC
Tru9I 338 TTAA T^TAA
Tsp45I 462 GTCAC ^GT[GC]AC
TthHB8I 624 TCGA T^CGA
XmaCI 8 CCCGGG C^CCGGG
XmaI 8 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 27 enzymes) #
###################################
BsiYI 142 CCTGAATACGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
205 CCTAAAACTGG
BslI 142 CCTGAATACGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
205 CCTAAAACTGG
BsmAI 292 GAGAC GTCTC(1/5)
700 GAGAC
BsmI 54 GAATGC GAATGC(1/-1)
441 GAATGC
BsrDI 519 CATTGC GCAATG(2/0)
717 CATTGC
BstAPI 361 GCAGACTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
663 GCACGTGGTGC
BstF5I 426 CATCC GGATG(2/0)
435 CATCC
BstNI 73 CCAGG CC^[AT]GG
588 CCTGG
CfoI 287 GCGC GCG^C
486 GCGC
DraII 94 AGGACCT [GA]G^G[ACGT]CC[CT]
222 AGGACCC
EarI 137 CTCTTC CTCTTC(1/4)
406 GAAGAG
EcoO109I 94 AGGACCT [GA]G^G[ACGT]CC[CT]
222 AGGACCC
EcoRII 73 CCAGG ^CC[AT]GG
588 CCTGG
FokI 426 CATCC GGATG(9/13)
435 CATCC
HapII 9 CCGG C^CGG
546 CCGG
HhaI 287 GCGC GCG^C
486 GCGC
HinP1I 287 GCGC G^CGC
486 GCGC
HpaII 9 CCGG C^CGG
546 CCGG
Ksp632I 137 CTCTTC CTCTTC(1/4)
406 GAAGAG
MfeI 446 CAATTG C^AATTG
704 CAATTG
MspI 9 CCGG C^CGG
546 CCGG
MunI 446 CAATTG C^AATTG
704 CAATTG
MvaI 73 CCAGG CC^[AT]GG
588 CCTGG
NlaIII 615 CATG CATG^
642 CATG
PpuMI 94 AGGACCT [GA]G^G[AT]CC[CT]
222 AGGACCC
SfaNI 618 GATGC GCATC(5/9)
677 GCATC
TspRI 300 CACTG CA[GC]TG[ACGT][ACGT]^
473 CACTG
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# 3 OR MORE CUT ENZYMES ( 26 enzymes) #
#########################################
AciI AfaI AvaII BbvI BcnI
BfaI BsaJI BsmFI BsrI BssKI
Cac8I Cfr13I Fnu4HI ItaI MaeI
MaeIII MboII MnlI MwoI NciI
NlaIV RsaI Sau96I ScrFI TseI
Tsp509I