Restriction map for
pFN21AE3882
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# NO CUT ENZYMES ( 152 enzymes) #
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AatII AccII AccIII AclI AcsI
AcyI AflII AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaLI ApoI
AscI AseI AsnI Asp700I AspEI
AspHI AvaI AvaII AviII AvrII
BalI BamHI BbeI BbrPI BbsI
BbvCI BcgI BcnI BfrI BlnI
BpuAI BsaAI BsaBI BsaHI BsaI
BseAI BseRI BsgI BsiEI BsiHKAI
BsiWI BsmBI BsoBI Bsp1407I BspDI
BspEI BspMI BsrBI BsrFI BsrGI
BssHII Bst1107I BstBI BstEII BstPI
BstUI BstXI BstZ17I Bsu36I Cac8I
CfoI Cfr10I ClaI CpoI DraIII
DrdI EaeI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoRI EcoT22I FseI FspI HaeII
HgaI HhaI Hin1I HinP1I HincII
HindII HpaI KasI Ksp632I KspI
MaeII MamI MluI MluNI MroI
MscI MslI MvnI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SphI Sse8387I SspBI StuI SwaI
TaqI TthHB8I XcmI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
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Acc65I 1216 GGTACC G^GTACC
AccI 406 GTAGAC GT^[AC][GT]AC
AfaI 1217 GTAC GT^AC
AflIII 73 ACATGT A^C[GA][CT]GT
ApaI 1262 GGGCCC GGGCC^C
Asp718I 1216 GGTACC G^GTACC
AspI 795 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanI 1216 GGTACC G^G[CT][GA]CC
BciVI 1044 GGATAC GTATCC(6/5)
BclI 1350 TGATCA T^GATCA
BglI 1255 GCCATTGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 1155 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1155 GCTCAGC GC^T[ACGT]AGC
BsaWI 732 TCCGGT [AT]^CCGG[AT]
BsmFI 1066 GTCCC GGGAC(10/14)
BsmI 36 GAATGC GAATGC(1/-1)
BspHI 471 TCATGA T^CATGA
BspLU11I 73 ACATGT A^CATGT
BssKI 26 CCTGG ^CC[ACGT]GG
BssSI 899 CTCGTG CACGAG(-5/-1)
BstAPI 517 GCAACTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstNI 26 CCTGG CC^[AT]GG
CelII 1155 GCTCAGC GC^T[ACGT]AGC
DraI 426 TTTAAA TTT^AAA
DsaI 180 CCGTGG C^C[GA][CT]GG
EcoNI 26 CCTGGAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRII 26 CCTGG ^CC[AT]GG
EcoRV 610 GATATC GAT^ATC
FauI 40 GCGGG CCCGC(4/6)
FbaI 1350 TGATCA T^GATCA
HapII 733 CCGG C^CGG
HpaII 733 CCGG C^CGG
HphI 658 TCACC GGTGA(8/7)
KpnI 1216 GGTACC GGTAC^C
MfeI 1208 CAATTG C^AATTG
MspA1I 1346 CAGCTG C[AC]G^C[GT]G
MspI 733 CCGG C^CGG
MunI 1208 CAATTG C^AATTG
MvaI 26 CCTGG CC^[AT]GG
NspI 73 ACATGT [GA]CATG^[CT]
PflFI 795 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 347 GAGTC GAGTC(4/5)
PvuII 1346 CAGCTG CAG^CTG
RcaI 471 TCATGA T^CATGA
RsaI 1217 GTAC GT^AC
ScrFI 26 CCTGG CC^[ACGT]GG
SfaNI 290 GCATC GCATC(5/9)
SspI 553 AATATT AAT^ATT
TfiI 433 GATTC G^A[AT]TC
Tsp45I 1380 GTCAC ^GT[GC]AC
Tth111I 795 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 372 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 23 enzymes) #
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AciI 1125 CCGC CCGC(-3/-1)
40 GCGG
AlwI 1204 GGATC GGATC(4/5)
1270 GGATC
BanII 1140 GGGCTC G[GA]GC[CT]^C
1262 GGGCCC
BglII 1088 AGATCT A^GATCT
1437 AGATCT
BmyI 1140 GGGCTC G[AGT]GC[ACT]^C
1262 GGGCCC
BpmI 1129 CTCCAG CTGGAG(16/14)
27 CTGGAG
BsmAI 276 GTCTC GTCTC(1/5)
468 GTCTC
Bsp1286I 1140 GGGCTC G[AGT]GC[ACT]^C
1262 GGGCCC
BstF5I 145 CATCC GGATG(2/0)
987 GGATG
BstYI 1088 AGATCT [GA]^GATC[CT]
1437 AGATCT
DraII 1261 GGGGCCC [GA]G^G[ACGT]CC[CT]
1262 GGGCCCT
EcoO109I 1261 GGGGCCC [GA]G^G[ACGT]CC[CT]
1262 GGGCCCT
EcoT14I 947 CCTTGG C^C[AT][AT]GG
1266 CCTTGG
FokI 145 CATCC GGATG(9/13)
987 GGATG
HindIII 314 AAGCTT A^AGCTT
422 AAGCTT
HinfI 347 GAGTC G^A[ACGT]TC
433 GATTC
MflI 1088 AGATCT [GA]^GATC[CT]
1437 AGATCT
MwoI 517 GCAACTCCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
1255 GCCATTGGGGC
PflMI 1131 CCAGAAATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1391 CCAATTTGTGG
SpeI 331 ACTAGT A^CTAGT
717 ACTAGT
StyI 947 CCTTGG C^C[AT][AT]GG
1266 CCTTGG
Van91I 1131 CCAGAAATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1391 CCAATTTGTGG
XhoII 1088 AGATCT [GA]^GATC[CT]
1437 AGATCT
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# 3 OR MORE CUT ENZYMES ( 31 enzymes) #
#########################################
AluI BbvI BfaI BsaJI BsiYI
BslI BsrDI BsrI Cfr13I DdeI
DpnII Fnu4HI HaeIII ItaI MaeI
MaeIII MboI MboII MnlI MseI
NdeII NlaIII NlaIV PstI Sau3AI
Sau96I SfcI Tru9I TseI Tsp509I
TspRI