Restriction map for
pFN21AE9395
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# NO CUT ENZYMES ( 148 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AcyI AflII AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AspI AvaI AvaII
AviII AvrII BamHI BanI BbeI
BbrPI BbvCI BcgI BciVI BclI
BfrI BglI BglII BlnI BlpI
Bpu1102I BsaAI BsaBI BsaHI BsaI
BsaWI BseAI BsiHKAI BsiWI BsmBI
BsmFI BsoBI Bsp1407I BspDI BspEI
BspHI BsrBI BsrDI BsrGI BssHII
BssSI Bst1107I BstAPI BstEII BstPI
BstUI BstXI BstZ17I Bsu36I CelII
CfoI ClaI CpoI DraI DraII
DraIII DrdI DsaI Eam1105I Eco47III
Eco81I EcoNI EcoO109I EcoO65I EcoRI
EcoT14I EcoT22I FbaI FseI FspI
HaeII HhaI Hin1I HinP1I HindIII
KasI KpnI KspI MamI MfeI
MluI MroI MunI MvnI NarI
NcoI NdeI NheI NotI NruI
NsiI PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI PstI PvuI RcaI RsrII
SacI SacII SalI SbfI SexAI
SfiI SfoI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI StuI
StyI SwaI Tth111I XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AclI 278 AACGTT AA^CGTT
AflIII 859 ACATGT A^C[GA][CT]GT
BcnI 41 CCGGG CC^[GC]GG
BseRI 199 GAGGAG GAGGAG(10/8)
BsiEI 30 CGGCCG CG[GA][CT]^CG
BsmAI 1114 GAGAC GTCTC(1/5)
BsmI 1706 GCATTC GAATGC(1/-1)
BspLU11I 859 ACATGT A^CATGT
BsrFI 28 GCCGGC [GA]^CCGG[CT]
BsrI 463 CCAGT ACTGG(1/-1)
BstBI 1607 TTCGAA TT^CGAA
BstYI 1242 AGATCC [GA]^GATC[CT]
Cac8I 28 GCCGGC GC[ACGT]^[ACGT]GC
Cfr10I 28 GCCGGC [GA]^CCGG[CT]
Cfr13I 16 GGGCC G^G[ACGT]CC
EagI 30 CGGCCG C^GGCCG
EclXI 30 CGGCCG C^GGCCG
Eco52I 30 CGGCCG C^GGCCG
EcoRV 577 GATATC GAT^ATC
FauI 50 CCCGC CCCGC(4/6)
HgaI 931 GCGTC GACGC(5/10)
HincII 1620 GTTAAC GT[CT]^[GA]AC
HindII 1620 GTTAAC GT[CT]^[GA]AC
HpaI 1620 GTTAAC GTT^AAC
HphI 1420 GGTGA GGTGA(8/7)
MaeII 279 ACGT A^CGT
MflI 1242 AGATCC [GA]^GATC[CT]
MslI 256 CACTACAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MwoI 4 GCGGACTTCGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NaeI 28 GCCGGC GCC^GGC
NciI 41 CCGGG CC^[GC]GG
NgoMIV 28 GCCGGC G^CCGGC
NspI 859 ACATGT [GA]CATG^[CT]
NspV 1607 TTCGAA TT^CGAA
PflMI 1696 CCAATTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Psp1406I 278 AACGTT AA^CGTT
SapI 1450 GAAGAGC GCTCTTC(1/4)
Sau96I 16 GGGCC G^G[ACGT]CC
ScaI 88 AGTACT AGT^ACT
SfcI 258 CTACAG C^T[GA][CT]AG
SfuI 1607 TTCGAA TT^CGAA
SmlI 1482 CTCAAG C^T[CT][GA]AG
Van91I 1696 CCAATTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1749 TCTAGA T^CTAGA
XhoII 1242 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AlwI 1243 GATCC GGATC(4/5)
1385 GGATC
BalI 525 TGGCCA TGG^CCA
1161 TGGCCA
BanII 44 GGGCTC G[GA]GC[CT]^C
1311 GGGCTC
BmyI 44 GGGCTC G[AGT]GC[ACT]^C
1311 GGGCTC
BsaJI 41 CCGGGG C^C[ACGT][ACGT]GG
1543 CCTGGG
Bsp1286I 44 GGGCTC G[AGT]GC[ACT]^C
1311 GGGCTC
DpnII 1243 GATC ^GATC
1386 GATC
HapII 29 CCGG C^CGG
41 CCGG
HpaII 29 CCGG C^CGG
41 CCGG
MboI 1243 GATC ^GATC
1386 GATC
MluNI 525 TGGCCA TGG^CCA
1161 TGGCCA
MscI 525 TGGCCA TGG^CCA
1161 TGGCCA
MspI 29 CCGG C^CGG
41 CCGG
NdeII 1243 GATC ^GATC
1386 GATC
NlaIII 860 CATG CATG^
1456 CATG
NlaIV 45 GGCTCC GG[ACGT]^[ACGT]CC
1554 GGTTCC
Sau3AI 1243 GATC ^GATC
1386 GATC
#########################################
# 3 OR MORE CUT ENZYMES ( 48 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
BbsI BbvI BfaI BpmI BpuAI
BsgI BsiYI BslI BspMI BssKI
BstF5I BstNI DdeI EaeI EarI
EcoRII Fnu4HI FokI HaeIII HinfI
ItaI Ksp632I MaeI MaeIII MboII
MnlI MseI MspA1I MvaI PleI
PvuII RsaI ScrFI SfaNI SspI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I