Restriction map for
pFN21AE2018
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# NO CUT ENZYMES ( 161 enzymes) #
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AatII Acc65I AccII AccIII AciI
AclI AcyI AgeI AhdI Alw44I
AlwNI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp718I AspEI AspHI
AspI AviII AvrII BalI BamHI
BanI BbeI BbrPI BbvCI BcgI
BciVI BcnI BglI BlnI BlpI
Bpu1102I BsaBI BsaHI BsaI BsaJI
BsaWI BseAI BseRI BsiEI BsiHKAI
BsiWI BsiYI BslI BsmBI BsmFI
BspDI BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssHII BssKI BssSI
Bst1107I BstAPI BstBI BstEII BstNI
BstPI BstUI BstZ17I Cac8I CelII
CfoI Cfr10I ClaI CpoI DraIII
DrdI DsaI EaeI EagI Eam1105I
EclXI Eco47III Eco52I EcoNI EcoO65I
EcoRI EcoRII EcoT14I FauI FseI
FspI HaeII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HpaI
HpaII HphI KasI KpnI KspI
MamI MluI MluNI MroI MscI
MspI MvaI MvnI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NlaIV NotI NruI NspV PacI
PflFI PflMI PinAI PmaCI PmeI
PmlI PshBI Psp1406I PstI PvuI
RcaI RsrII SacI SacII SalI
SbfI ScrFI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I StyI SwaI Tsp45I
Tth111I Van91I XbaI XcmI XmaCI
XmaI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AocI 1093 CCTCAGG CC^T[ACGT]AGG
AvaI 1718 CTCGAG C^[CT]CG[GA]G
BanII 334 GAGCCC G[GA]GC[CT]^C
BbsI 1039 GAAGAC GAAGAC(2/6)
BclI 900 TGATCA T^GATCA
BglII 1602 AGATCT A^GATCT
BpmI 1015 CTGGAG CTGGAG(16/14)
BpuAI 1039 GAAGAC GAAGAC(2/6)
BsaAI 1304 CACGTA [CT]AC^GT[GA]
BsgI 1376 GTGCAG GTGCAG(16/14)
BsmAI 1132 GAGAC GTCTC(1/5)
BsmI 1674 GCATTC GAATGC(1/-1)
BsoBI 1718 CTCGAG C^[CT]CG[GA]G
Bsp1407I 882 TGTACA T^GTACA
BspLU11I 1286 ACATGT A^CATGT
BsrGI 882 TGTACA T^GTACA
BstXI 998 CCAAAGGATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1093 CCTCAGG CC^T[ACGT]AGG
DraI 61 TTTAAA TTT^AAA
DraII 1667 AGGTCCT [GA]G^G[ACGT]CC[CT]
Eco81I 1093 CCTCAGG CC^T[ACGT]AGG
EcoO109I 1667 AGGTCCT [GA]G^G[ACGT]CC[CT]
EcoRV 351 GATATC GAT^ATC
EcoT22I 105 ATGCAT ATGCA^T
FbaI 900 TGATCA T^GATCA
HindIII 642 AAGCTT A^AGCTT
MaeII 1305 ACGT A^CGT
MaeIII 1307 GTAAC ^GT[ACGT]AC
MslI 1237 CACTCAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 1165 CAGCTG C[AC]G^C[GT]G
NsiI 105 ATGCAT ATGCA^T
NspI 1286 ACATGT [GA]CATG^[CT]
PaeR7I 1718 CTCGAG C^TCGAG
PpuMI 1667 AGGTCCT [GA]G^G[AT]CC[CT]
PshAI 1042 GACAATAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 1165 CAGCTG CAG^CTG
ScaI 1061 AGTACT AGT^ACT
SfcI 5 CTGTAG C^T[GA][CT]AG
SspBI 882 TGTACA T^GTACA
StuI 533 AGGCCT AGG^CCT
XhoI 1718 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AflII 918 CTTAAG C^TTAAG
1663 CTTAAG
AflIII 1286 ACATGT A^C[GA][CT]GT
1303 ACACGT
AlwI 703 GATCC GGATC(4/5)
1635 GATCC
BbvI 1659 GCAGC GCAGC(8/12)
1011 GCTGC
BfrI 918 CTTAAG C^TTAAG
1663 CTTAAG
BstYI 1602 AGATCT [GA]^GATC[CT]
1634 AGATCC
Fnu4HI 1011 GCTGC GC^[ACGT]GC
1659 GCAGC
ItaI 1011 GCTGC GC^[ACGT]GC
1659 GCAGC
MfeI 425 CAATTG C^AATTG
749 CAATTG
MflI 1602 AGATCT [GA]^GATC[CT]
1634 AGATCC
MunI 425 CAATTG C^AATTG
749 CAATTG
MwoI 644 GCTTGAAATGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
859 GCTTCTCTGGC
NlaIII 784 CATG CATG^
1287 CATG
PleI 850 GACTC GAGTC(4/5)
985 GAGTC
SapI 49 GAAGAGC GCTCTTC(1/4)
331 GAAGAGC
SspI 1498 AATATT AAT^ATT
1768 AATATT
TaqI 210 TCGA T^CGA
1719 TCGA
TseI 1011 GCTGC G^C[AT]GC
1659 GCAGC
TthHB8I 210 TCGA T^CGA
1719 TCGA
XhoII 1602 AGATCT [GA]^GATC[CT]
1634 AGATCC
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# 3 OR MORE CUT ENZYMES ( 36 enzymes) #
#########################################
AccI AcsI AfaI AluI ApoI
Asp700I AvaII BfaI BmyI Bsp1286I
BsrI BstF5I Cfr13I DdeI DpnII
EarI FokI HaeIII HinfI Ksp632I
MaeI MboI MboII MnlI MseI
NdeII RsaI Sau3AI Sau96I SfaNI
SmlI TfiI Tru9I Tsp509I TspRI
XmnI