Restriction map for
pFN21AB7642
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# NO CUT ENZYMES ( 120 enzymes) #
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AatII AccI AccIII AclI AcsI
AflII AflIII AgeI AhdI AocI
ApaI ApoI AscI AseI AsnI
Asp700I AspEI AspI AviII BalI
BamHI BbrPI BbvCI BcgI BclI
BfrI BglII BlpI Bpu1102I BsaAI
BsaBI BseAI BsiWI BsmBI BsmI
BspDI BspEI BspHI BspLU11I BspMI
BsrDI BssHII Bst1107I BstBI BstEII
BstPI BstXI BstYI BstZ17I Bsu36I
CelII ClaI CpoI DraI DraIII
Eam1105I EarI Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HincII HindII HindIII HpaI
Ksp632I MamI MfeI MflI MluI
MluNI MroI MscI MslI MunI
NdeI NheI NotI NruI NsiI
NspV PflFI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI SalI
SapI SbfI ScaI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspI StuI SwaI Tth111I XbaI
XcmI XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 55 enzymes) #
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Acc65I 836 GGTACC G^GTACC
AcyI 180 GGCGCC G[GA]^CG[CT]C
AlwI 780 GGATC GGATC(4/5)
Aor51HI 801 AGCGCT AGC^GCT
Asp718I 836 GGTACC G^GTACC
AvaI 556 CTCGAG C^[CT]CG[GA]G
AvrII 1092 CCTAGG C^CTAGG
BbeI 180 GGCGCC GGCGC^C
BbsI 417 GTCTTC GAAGAC(2/6)
BciVI 490 GTATCC GTATCC(6/5)
BfaI 1093 CTAG C^TAG
BlnI 1092 CCTAGG C^CTAGG
BpuAI 417 GTCTTC GAAGAC(2/6)
BsaHI 180 GGCGCC G[GA]^CG[CT]C
BsaI 641 GAGACC GGTCTC(1/5)
BsaWI 1025 ACCGGA [AT]^CCGG[AT]
BseRI 1031 CTCCTC GAGGAG(10/8)
BsgI 775 GTGCAG GTGCAG(16/14)
BsmAI 641 GAGAC GTCTC(1/5)
BsoBI 556 CTCGAG C^[CT]CG[GA]G
Bsp1407I 1010 TGTACA T^GTACA
BsrFI 288 GCCGGC [GA]^CCGG[CT]
BsrGI 1010 TGTACA T^GTACA
BssSI 535 CACGAG CACGAG(-5/-1)
Cfr10I 288 GCCGGC [GA]^CCGG[CT]
DpnII 781 GATC ^GATC
DraII 1095 AGGGCCT [GA]G^G[ACGT]CC[CT]
Eco47III 801 AGCGCT AGC^GCT
EcoO109I 1095 AGGGCCT [GA]G^G[ACGT]CC[CT]
Hin1I 180 GGCGCC G[GA]^CG[CT]C
KasI 180 GGCGCC G^GCGCC
KpnI 836 GGTACC GGTAC^C
MaeI 1093 CTAG C^TAG
MaeII 415 ACGT A^CGT
MaeIII 202 GTCAC ^GT[ACGT]AC
MboI 781 GATC ^GATC
NaeI 288 GCCGGC GCC^GGC
NarI 180 GGCGCC GG^CGCC
NcoI 206 CCATGG C^CATGG
NdeII 781 GATC ^GATC
NgoMIV 288 GCCGGC G^CCGGC
PacI 427 TTAATTAA TTAAT^TAA
PaeR7I 556 CTCGAG C^TCGAG
PflMI 375 CCAGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Sau3AI 781 GATC ^GATC
SexAI 832 ACCTGGT A^CC[AT]GGT
SfiI 390 GGCCGCCTCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 180 GGCGCC GGC^GCC
SmlI 556 CTCGAG C^T[CT][GA]AG
SspBI 1010 TGTACA T^GTACA
TfiI 598 GATTC G^A[AT]TC
Tsp45I 202 GTCAC ^GT[GC]AC
Tsp509I 429 AATT ^AATT
Van91I 375 CCAGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 556 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
Alw44I 470 GTGCAC G^TGCAC
532 GTGCAC
AlwNI 337 CAGCCGCTG CAG[ACGT][ACGT][ACGT]^CTG
376 CAGCAGCTG
ApaLI 470 GTGCAC G^TGCAC
532 GTGCAC
BanII 211 GAGCCC G[GA]GC[CT]^C
384 GGGCTC
BglI 391 GCCGCCTCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
959 GCCTGCTGGGC
BsiEI 290 CGGCCG CG[GA][CT]^CG
389 CGGCCG
BsrBI 100 CCGCTC CCGCTC(-3/-3)
754 GAGCGG
DdeI 198 CTCAG C^T[ACGT]AG
769 CTGAG
DrdI 408 GACTTCCACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
817 GACACCCAAGTC
EagI 290 CGGCCG C^GGCCG
389 CGGCCG
EclXI 290 CGGCCG C^GGCCG
389 CGGCCG
Eco52I 290 CGGCCG C^GGCCG
389 CGGCCG
EcoT14I 206 CCATGG C^C[AT][AT]GG
1092 CCTAGG
HgaI 238 GACGC GACGC(5/10)
303 GACGC
KspI 119 CCGCGG CCGC^GG
293 CCGCGG
MboII 187 TCTTC GAAGA(8/7)
418 TCTTC
NspI 466 GCATGT [GA]CATG^[CT]
1019 GCATGT
PvuII 379 CAGCTG CAG^CTG
1051 CAGCTG
SacII 119 CCGCGG CCGC^GG
293 CCGCGG
SfaNI 225 GCATC GCATC(5/9)
249 GCATC
StyI 206 CCATGG C^C[AT][AT]GG
1092 CCTAGG
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# 3 OR MORE CUT ENZYMES ( 62 enzymes) #
#########################################
AccII AciI AfaI AluI AspHI
AvaII BanI BbvI BcnI BmyI
BpmI BsaJI BsiHKAI BsiYI BslI
BsmFI Bsp1286I BsrI BssKI BstAPI
BstF5I BstNI BstUI Cac8I CfoI
Cfr13I DsaI EaeI EcoRII FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI MnlI MseI MspA1I MspI
MvaI MvnI MwoI NciI NlaIII
NlaIV PleI PstI RsaI Sau96I
ScrFI SfcI TaqI Tru9I TseI
TspRI TthHB8I