Restriction map for
pFN21AE2537
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# NO CUT ENZYMES ( 128 enzymes) #
##################################
Acc65I AclI AfaI AflIII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp718I AspEI AspHI AviII AvrII
BalI BamHI BbrPI BbvCI BcgI
BclI BglI BglII BlnI BlpI
BpmI Bpu1102I BsaBI BsaI BsiHKAI
BsiWI BsmFI BsmI Bsp1407I BspDI
BspHI BspLU11I BsrBI BsrDI BsrGI
BssHII BssSI Bst1107I BstBI BstEII
BstNI BstPI BstXI BstYI BstZ17I
Bsu36I CelII ClaI CpoI DraI
Eam1105I Eco47III Eco81I EcoNI EcoO65I
EcoRI EcoRII EcoRV EcoT22I FbaI
FseI FspI HindIII HpaI KpnI
KspI MamI MflI MluI MluNI
MscI MslI MspA1I MvaI NcoI
NheI NotI NruI NsiI NspV
PaeR7I PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
PvuII RcaI RsaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfaNI SfiI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI TaqI TthHB8I
XbaI XhoI XhoII
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# ONE CUT ENZYMES ( 60 enzymes) #
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AatII 115 GACGTC GACGT^C
AccI 847 GTAGAC GT^[AC][GT]AC
AccIII 213 TCCGGA T^CCGGA
AcsI 409 GAATTT [GA]^AATT[CT]
AflII 592 CTTAAG C^TTAAG
AlwI 771 GATCC GGATC(4/5)
ApoI 409 GAATTT [GA]^AATT[CT]
AspI 217 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 759 GGACC G^G[AT]CC
BanI 282 GGCGCC G^G[CT][GA]CC
BanII 7 GGGCTC G[GA]GC[CT]^C
BbeI 282 GGCGCC GGCGC^C
BbsI 223 GTCTTC GAAGAC(2/6)
BciVI 723 GTATCC GTATCC(6/5)
BfaI 569 CTAG C^TAG
BfrI 592 CTTAAG C^TTAAG
BpuAI 223 GTCTTC GAAGAC(2/6)
BsaAI 148 TACGTG [CT]AC^GT[GA]
BsaWI 213 TCCGGA [AT]^CCGG[AT]
BseAI 213 TCCGGA T^CCGGA
BsmBI 256 GAGACG CGTCTC(1/5)
BspEI 213 TCCGGA T^CCGGA
BsrFI 195 GCCGGC [GA]^CCGG[CT]
BstAPI 693 GCACGTCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 804 GGATG GGATG(2/0)
Cfr10I 195 GCCGGC [GA]^CCGG[CT]
DraII 587 AGGCCCT [GA]G^G[ACGT]CC[CT]
DraIII 43 CACGCCGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 478 GACTATGCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I 587 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoT14I 98 CCAAGG C^C[AT][AT]GG
FokI 804 GGATG GGATG(9/13)
HgaI 221 GCGTC GACGC(5/10)
HincII 304 GTTGAC GT[CT]^[GA]AC
HindII 304 GTTGAC GT[CT]^[GA]AC
KasI 282 GGCGCC G^GCGCC
MaeI 569 CTAG C^TAG
MaeIII 614 GTGAC ^GT[ACGT]AC
MfeI 560 CAATTG C^AATTG
MroI 213 TCCGGA T^CCGGA
MunI 560 CAATTG C^AATTG
NaeI 195 GCCGGC GCC^GGC
NarI 282 GGCGCC GG^CGCC
NdeI 763 CATATG CA^TATG
NgoMIV 195 GCCGGC G^CCGGC
NlaIV 282 GGCGCC GG[ACGT]^[ACGT]CC
NspI 782 GCATGT [GA]CATG^[CT]
PacI 832 TTAATTAA TTAAT^TAA
PflFI 217 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 42 CCACGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SfoI 282 GGCGCC GGC^GCC
SmaI 277 CCCGGG CCC^GGG
SmlI 592 CTTAAG C^T[CT][GA]AG
StyI 98 CCAAGG C^C[AT][AT]GG
TfiI 574 GAATC G^A[AT]TC
Tsp45I 614 GTGAC ^GT[GC]AC
Tth111I 217 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 42 CCACGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 277 CCCGGG C^CCGGG
XmaI 277 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AcyI 115 GACGTC G[GA]^CG[CT]C
282 GGCGCC
Asp700I 328 GAAAAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
522 GAAGAGTTTC
AvaI 64 CCCGAG C^[CT]CG[GA]G
277 CCCGGG
BmyI 7 GGGCTC G[AGT]GC[ACT]^C
691 GGGCAC
BsaHI 115 GACGTC G[GA]^CG[CT]C
282 GGCGCC
BseRI 106 GAGGAG GAGGAG(10/8)
253 GAGGAG
BsgI 578 CTGCAC GTGCAG(16/14)
703 CTGCAC
BsiEI 30 CGGCCG CG[GA][CT]^CG
197 CGGCCG
BsmAI 256 GAGAC GTCTC(1/5)
487 GTCTC
BsoBI 64 CCCGAG C^[CT]CG[GA]G
277 CCCGGG
Bsp1286I 7 GGGCTC G[AGT]GC[ACT]^C
691 GGGCAC
BspMI 817 ACCTGC ACCTGC(4/8)
180 GCAGGT
BsrI 792 ACTGG ACTGG(1/-1)
499 CCAGT
DdeI 88 CTGAG C^T[ACGT]AG
825 CTCAG
DsaI 47 CCGTGG C^C[GA][CT]GG
242 CCGTGG
EagI 30 CGGCCG C^GGCCG
197 CGGCCG
EarI 522 GAAGAG CTCTTC(1/4)
718 GAAGAG
EclXI 30 CGGCCG C^GGCCG
197 CGGCCG
Eco52I 30 CGGCCG C^GGCCG
197 CGGCCG
Hin1I 115 GACGTC G[GA]^CG[CT]C
282 GGCGCC
HphI 269 TCACC GGTGA(8/7)
815 TCACC
Ksp632I 522 GAAGAG CTCTTC(1/4)
718 GAAGAG
PleI 850 GACTC GAGTC(4/5)
67 GAGTC
PstI 178 CTGCAG CTGCA^G
531 CTGCAG
SfcI 178 CTGCAG C^T[GA][CT]AG
531 CTGCAG
XcmI 38 CCACCCACGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
491 CCACAGTGCCAGTGG
XmnI 328 GAAAAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
522 GAAGAGTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 43 enzymes) #
#########################################
AccII AciI AluI BbvI BcnI
BsaJI BsiYI BslI BssKI BstUI
Cac8I CfoI Cfr13I DpnII EaeI
FauI Fnu4HI HaeII HaeIII HapII
HhaI HinP1I HinfI HpaII ItaI
MaeII MboI MboII MnlI MseI
MspI MvnI MwoI NciI NdeII
NlaIII Sau3AI Sau96I ScrFI Tru9I
TseI Tsp509I TspRI