##################################
# NO CUT ENZYMES (  217 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AcyI        AflII       AflIII      AgeI        
AhdI        Alw44I      AlwI        AlwNI       AocI        
Aor51HI     ApaI        ApaLI       ApoI        AscI        
AseI        AsnI        Asp718I     AspEI       AspHI       
AspI        AvaI        AvaII       AviII       AvrII       
BalI        BamHI       BanI        BanII       BbeI        
BbrPI       BbsI        BbvCI       BcgI        BciVI       
BclI        BcnI        BfaI        BfrI        BglI        
BglII       BlnI        BlpI        BmyI        Bpu1102I    
BpuAI       BsaAI       BsaBI       BsaHI       BsaI        
BsaWI       BseAI       BsgI        BsiEI       BsiHKAI     
BsiWI       BsmAI       BsmBI       BsmFI       BsoBI       
Bsp1286I    Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BspMI       BsrBI       BsrDI       BsrFI       
BsrGI       BssHII      BssSI       Bst1107I    BstAPI      
BstBI       BstEII      BstF5I      BstPI       BstXI       
BstYI       BstZ17I     Bsu36I      Cac8I       CelII       
CfoI        Cfr10I      Cfr13I      ClaI        CpoI        
DdeI        DpnII       DraI        DraII       DraIII      
DrdI        DsaI        EaeI        EagI        Eam1105I    
EarI        EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoO109I    EcoO65I     EcoRI       EcoRV       
EcoT14I     EcoT22I     FauI        FbaI        FokI        
FseI        FspI        HaeII       HhaI        Hin1I       
HinP1I      HincII      HindII      HindIII     HpaI        
HphI        KasI        KpnI        Ksp632I     KspI        
MaeI        MaeII       MaeIII      MamI        MboI        
MfeI        MflI        MluI        MluNI       MroI        
MscI        MseI        MslI        MunI        NaeI        
NarI        NciI        NcoI        NdeI        NdeII       
NgoMIV      NheI        NotI        NruI        NsiI        
NspI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       Psp1406I    PvuI        
RcaI        RsrII       SacI        SacII       SalI        
SapI        Sau3AI      Sau96I      SbfI        ScaI        
SexAI       SfaNI       SfiI        SfoI        SfuI        
SgrAI       SmaI        SmlI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        StuI        
StyI        SwaI        TaqI        TfiI        Tru9I       
Tsp45I      Tsp509I     TspRI       Tth111I     TthHB8I     
Van91I      XbaI        XcmI        XhoI        XhoII       
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   27 enzymes) #
###################################

AccII          73  CGCG                 CG^CG
AfaI          164  GTAC                 GT^AC
Asp700I       105  GAAACAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BsaJI          86  CCCAGG               C^C[ACGT][ACGT]GG
BseRI           9  CTCCTC               GAGGAG(10/8)
BsiYI          51  CCGGCTGGAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI           51  CCGGCTGGAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmI          123  GAATGC               GAATGC(1/-1)
BsrI          148  ACTGG                ACTGG(1/-1)
BssKI          87  CCAGG                ^CC[ACGT]GG
BstNI          87  CCAGG                CC^[AT]GG
BstUI          73  CGCG                 CG^CG
EcoRII         87  CCAGG                ^CC[AT]GG
HaeIII         60  GGCC                 GG^CC
HgaI           17  GCGTC                GACGC(5/10)
HinfI          65  GACTC                G^A[ACGT]TC
MspA1I         92  CAGCTG               C[AC]G^C[GT]G
MvaI           87  CCAGG                CC^[AT]GG
MvnI           73  CGCG                 CG^CG
MwoI            8  GCTCCTCCAGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NlaIII        133  CATG                 CATG^
NlaIV           7  GGCTCC               GG[ACGT]^[ACGT]CC
PleI           65  GACTC                GAGTC(4/5)
PvuII          92  CAGCTG               CAG^CTG
RsaI          164  GTAC                 GT^AC
ScrFI          87  CCAGG                CC^[ACGT]GG
XmnI          105  GAAACAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   10 enzymes) #
###################################

AciI           23  CCGC                 CCGC(-3/-1)
               72  CCGC                
AluI           47  AGCT                 AG^CT
               93  AGCT                
BbvI           91  GCAGC                GCAGC(8/12)
               94  GCTGC               
HapII          51  CCGG                 C^CGG
               62  CCGG                
HpaII          51  CCGG                 C^CGG
               62  CCGG                
MboII          30  GAAGA                GAAGA(8/7)
              157  TCTTC               
MspI           51  CCGG                 C^CGG
               62  CCGG                
PstI           95  CTGCAG               CTGCA^G
              118  CTGCAG              
SfcI           95  CTGCAG               C^T[GA][CT]AG
              118  CTGCAG              
TseI           91  GCAGC                G^C[AT]GC
               94  GCTGC               


#########################################
# 3 OR MORE CUT ENZYMES (    4 enzymes) #
#########################################

BpmI        Fnu4HI      ItaI        MnlI