Restriction map for
pFN21AE5054
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# NO CUT ENZYMES ( 195 enzymes) #
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AatII Acc65I AccII AccIII AciI
AclI AcyI AflII AgeI AhdI
AlwI AlwNI AocI Aor51HI ApaI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvaI AvaII AviII
AvrII BalI BamHI BanII BbeI
BbrPI BbvCI BciVI BclI BcnI
BfaI BfrI BglII BlnI BlpI
BpmI Bpu1102I BsaAI BsaBI BsaHI
BsaWI BseAI BsgI BsiEI BsiWI
BsmBI BsmI BsoBI Bsp1407I BspDI
BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BsrI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstXI BstYI BstZ17I Bsu36I
CelII CfoI Cfr10I ClaI CpoI
DdeI DraI DraII DraIII DrdI
DsaI EaeI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRI EcoRV EcoT14I
EcoT22I FauI FbaI FseI FspI
HaeII HapII HgaI HhaI Hin1I
HinP1I HindIII HpaII KasI KpnI
Ksp632I KspI MaeI MaeII MaeIII
MamI MfeI MflI MluI MluNI
MroI MscI MslI MspI MunI
MvnI NaeI NarI NciI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfaNI SfcI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
StyI SwaI TaqI Tsp45I TspRI
Tth111I TthHB8I Van91I XbaI XcmI
XhoI XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 36 enzymes) #
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AccI 229 GTCTAC GT^[AC][GT]AC
AfaI 395 GTAC GT^AC
AflIII 334 ACATGT A^C[GA][CT]GT
Alw44I 12 GTGCAC G^TGCAC
ApaLI 12 GTGCAC G^TGCAC
AspHI 12 GTGCAC G[AT]GC[AT]^C
BanI 316 GGCACC G^G[CT][GA]CC
BbsI 153 GAAGAC GAAGAC(2/6)
BcgI 120 CGACATCTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 133 GCCTGGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 153 GAAGAC GAAGAC(2/6)
BsaI 344 GAGACC GGTCTC(1/5)
BseRI 49 CTCCTC GAGGAG(10/8)
BsiHKAI 12 GTGCAC G[AT]GC[AT]^C
BsiYI 398 CCCAGAATTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 398 CCCAGAATTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 344 GAGAC GTCTC(1/5)
BsmFI 75 GGGAC GGGAC(10/14)
BspLU11I 334 ACATGT A^CATGT
BstF5I 137 GGATG GGATG(2/0)
DpnII 286 GATC ^GATC
FokI 137 GGATG GGATG(9/13)
HincII 165 GTTAAC GT[CT]^[GA]AC
HindII 165 GTTAAC GT[CT]^[GA]AC
HpaI 165 GTTAAC GTT^AAC
HphI 53 TCACC GGTGA(8/7)
MboI 286 GATC ^GATC
MboII 153 GAAGA GAAGA(8/7)
MspA1I 7 CAGCTG C[AC]G^C[GT]G
MwoI 133 GCCTGGATGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NdeII 286 GATC ^GATC
NspI 334 ACATGT [GA]CATG^[CT]
PleI 357 GAGTC GAGTC(4/5)
PvuII 7 CAGCTG CAG^CTG
RsaI 395 GTAC GT^AC
Sau3AI 286 GATC ^GATC
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# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AcsI 82 AAATTC [GA]^AATT[CT]
410 AAATTT
ApoI 82 AAATTC [GA]^AATT[CT]
410 AAATTT
BbvI 130 GCAGC GCAGC(8/12)
180 GCAGC
BmyI 12 GTGCAC G[AGT]GC[ACT]^C
315 GGGCAC
Bsp1286I 12 GTGCAC G[AGT]GC[ACT]^C
315 GGGCAC
Cac8I 22 GCCAGC GC[ACGT]^[ACGT]GC
44 GCAGGC
Cfr13I 20 GGGCC G^G[ACGT]CC
69 GGCCC
Fnu4HI 130 GCAGC GC^[ACGT]GC
180 GCAGC
HaeIII 21 GGCC GG^CC
69 GGCC
ItaI 130 GCAGC GC^[ACGT]GC
180 GCAGC
MseI 166 TTAA T^TAA
248 TTAA
NlaIV 47 GGCTCC GG[ACGT]^[ACGT]CC
316 GGCACC
Sau96I 20 GGGCC G^G[ACGT]CC
69 GGCCC
Tru9I 166 TTAA T^TAA
248 TTAA
TseI 130 GCAGC G^C[AT]GC
180 GCAGC
#########################################
# 3 OR MORE CUT ENZYMES ( 12 enzymes) #
#########################################
AluI BsaJI BssKI BstNI EcoRII
HinfI MnlI MvaI NlaIII ScrFI
TfiI Tsp509I