Restriction map for
pFN21AE3881
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# NO CUT ENZYMES ( 155 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AlwI AocI Aor51HI
ApaI ApaLI AscI Asp718I AspEI
AspI AvaI AvrII BamHI BanI
BanII BbeI BbrPI BbvCI BcgI
BciVI BclI BfrI BglI BlnI
BlpI BpmI Bpu1102I BsaAI BsaHI
BsaI BsaJI BsaWI BseAI BseRI
BsiEI BsiWI BsmBI BsmFI BsoBI
Bsp1407I BspDI BspEI BspLU11I BspMI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstNI BstPI
BstUI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DrdI DsaI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoRII
EcoT14I EcoT22I FbaI FseI HaeII
HgaI Hin1I HincII HindII HindIII
HpaI KasI KpnI Ksp632I KspI
MaeII MluI MroI MspA1I MvaI
MvnI NaeI NarI NcoI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI Psp1406I PvuI PvuII RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI StuI StyI SwaI
Tth111I Van91I XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 57 enzymes) #
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AfaI 146 GTAC GT^AC
AluI 50 AGCT AG^CT
AlwNI 1270 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG
AseI 208 ATTAAT AT^TAAT
AsnI 208 ATTAAT AT^TAAT
AspHI 526 GAGCAC G[AT]GC[AT]^C
AviII 44 TGCGCA TGC^GCA
BbsI 74 GTCTTC GAAGAC(2/6)
BcnI 1146 CCGGG CC^[GC]GG
BglII 1126 AGATCT A^GATCT
BmyI 526 GAGCAC G[AGT]GC[ACT]^C
BpuAI 74 GTCTTC GAAGAC(2/6)
BsaBI 31 GATGAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI 1259 CTGCAC GTGCAG(16/14)
BsiHKAI 526 GAGCAC G[AT]GC[AT]^C
BsmAI 557 GAGAC GTCTC(1/5)
Bsp1286I 526 GAGCAC G[AGT]GC[ACT]^C
BspHI 1151 TCATGA T^CATGA
BsrBI 718 GAGCGG CCGCTC(-3/-3)
BssKI 1146 CCGGG ^CC[ACGT]GG
BstAPI 1269 GCAGATGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 669 CCATGTGACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 1126 AGATCT [GA]^GATC[CT]
CfoI 45 GCGC GCG^C
DpnII 1127 GATC ^GATC
DraI 1113 TTTAAA TTT^AAA
DraII 565 AGGCCCT [GA]G^G[ACGT]CC[CT]
DraIII 949 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoO109I 565 AGGCCCT [GA]G^G[ACGT]CC[CT]
FauI 1278 GCGGG CCCGC(4/6)
FspI 44 TGCGCA TGC^GCA
HapII 1146 CCGG C^CGG
HhaI 45 GCGC GCG^C
HinP1I 45 GCGC G^CGC
HpaII 1146 CCGG C^CGG
MamI 31 GATGAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboI 1127 GATC ^GATC
MfeI 1328 CAATTG C^AATTG
MflI 1126 AGATCT [GA]^GATC[CT]
MslI 664 CATGGCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 1146 CCGG C^CGG
MunI 1328 CAATTG C^AATTG
NciI 1146 CCGGG CC^[GC]GG
NdeI 1055 CATATG CA^TATG
NdeII 1127 GATC ^GATC
PleI 72 GAGTC GAGTC(4/5)
PshBI 208 ATTAAT AT^TAAT
PstI 1283 CTGCAG CTGCA^G
RcaI 1151 TCATGA T^CATGA
RsaI 146 GTAC GT^AC
Sau3AI 1127 GATC ^GATC
ScrFI 1146 CCGGG CC^[ACGT]GG
SmlI 1312 CTTGAG C^T[CT][GA]AG
SspI 1189 AATATT AAT^ATT
XbaI 393 TCTAGA T^CTAGA
XcmI 1187 CCAATATTTTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 1126 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AciI 720 GCGG CCGC(-3/-1)
1278 GCGG
AcsI 386 AAATTT [GA]^AATT[CT]
590 AAATTC
ApoI 386 AAATTT [GA]^AATT[CT]
590 AAATTC
Asp700I 59 GAAATGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
460 GAAGTTTTTC
AvaII 610 GGTCC G^G[AT]CC
1230 GGACC
BsiYI 686 CCGTAGTGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1233 CCCCATGAAGG
BslI 686 CCGTAGTGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1233 CCCCATGAAGG
BsmI 906 GCATTC GAATGC(1/-1)
1175 GCATTC
Cac8I 47 GCAAGC GC[ACGT]^[ACGT]GC
1278 GCGGGC
EcoRV 700 GATATC GAT^ATC
869 GATATC
HphI 704 TCACC GGTGA(8/7)
920 TCACC
NlaIV 609 GGGTCC GG[ACGT]^[ACGT]CC
1230 GGACCC
SfaNI 128 GATGC GCATC(5/9)
1272 GATGC
TfiI 241 GATTC G^A[AT]TC
945 GATTC
XmnI 59 GAAATGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
460 GAAGTTTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 31 enzymes) #
#########################################
BalI BbvI BfaI BsrI BstF5I
Cfr13I DdeI EaeI Fnu4HI FokI
HaeIII HinfI ItaI MaeI MaeIII
MboII MluNI MnlI MscI MseI
MwoI NlaIII Sau96I SfcI TaqI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I