Restriction map for
pFN21AE6359
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# NO CUT ENZYMES ( 163 enzymes) #
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AatII Acc65I AccIII AclI AcyI
AflII AgeI AhdI Alw44I AlwNI
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp718I AspEI AspHI
AspI AvaI AvaII AviII AvrII
BanI BanII BbeI BbrPI BcgI
BciVI BclI BfrI BglI BglII
BlnI BlpI BpmI Bpu1102I BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BsgI BsiEI BsiHKAI BsiWI BsmBI
BsmI BsoBI BspDI BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstXI BstZ17I Bsu36I CelII
CfoI Cfr10I Cfr13I ClaI CpoI
DraI DraII DraIII DrdI DsaI
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoO109I EcoO65I EcoRI
EcoRV EcoT22I FauI FbaI FseI
FspI HaeII HhaI Hin1I HinP1I
HincII HindII HindIII HpaI KasI
KpnI Ksp632I KspI MamI MluI
MroI MslI MspA1I MwoI NaeI
NarI NcoI NgoMIV NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
PvuII RcaI RsrII SacI SacII
SalI SapI Sau96I SbfI SexAI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
StuI SwaI Tth111I XbaI XcmI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AccI 843 GTCTAC GT^[AC][GT]AC
AccII 549 CGCG CG^CG
AflIII 180 ACATGT A^C[GA][CT]GT
Asp700I 1039 GAACAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 120 TGGCCA TGG^CCA
BbvCI 18 CCTCAGC CCTCAGC(-5/-2)
BcnI 24 CCGGG CC^[GC]GG
BmyI 1124 GGGCAC G[AGT]GC[ACT]^C
BseRI 428 CTCCTC GAGGAG(10/8)
BsmAI 943 GAGAC GTCTC(1/5)
BsmFI 824 GTCCC GGGAC(10/14)
Bsp1286I 1124 GGGCAC G[AGT]GC[ACT]^C
Bsp1407I 151 TGTACA T^GTACA
BspLU11I 180 ACATGT A^CATGT
BsrGI 151 TGTACA T^GTACA
BstUI 549 CGCG CG^CG
EcoNI 810 CCTTTTTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 618 CCAAGG C^C[AT][AT]GG
HapII 24 CCGG C^CGG
HgaI 581 GACGC GACGC(5/10)
HpaII 24 CCGG C^CGG
HphI 8 TCACC GGTGA(8/7)
MaeII 441 ACGT A^CGT
MfeI 719 CAATTG C^AATTG
MluNI 120 TGGCCA TGG^CCA
MscI 120 TGGCCA TGG^CCA
MspI 24 CCGG C^CGG
MunI 719 CAATTG C^AATTG
MvnI 549 CGCG CG^CG
NciI 24 CCGGG CC^[GC]GG
NdeI 1168 CATATG CA^TATG
NheI 787 GCTAGC G^CTAGC
NspI 180 ACATGT [GA]CATG^[CT]
PflMI 729 CCATCTTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 725 GAGTC GAGTC(4/5)
ScaI 424 AGTACT AGT^ACT
SspBI 151 TGTACA T^GTACA
SspI 466 AATATT AAT^ATT
StyI 618 CCAAGG C^C[AT][AT]GG
Van91I 729 CCATCTTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 1039 GAACAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 16 enzymes) #
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AciI 77 CCGC CCGC(-3/-1)
550 GCGG
BamHI 413 GGATCC G^GATCC
879 GGATCC
BbsI 994 GAAGAC GAAGAC(2/6)
443 GTCTTC
BpuAI 994 GAAGAC GAAGAC(2/6)
443 GTCTTC
BstNI 850 CCTGG CC^[AT]GG
1099 CCTGG
BstYI 413 GGATCC [GA]^GATC[CT]
879 GGATCC
Cac8I 255 GCTTGC GC[ACGT]^[ACGT]GC
787 GCTAGC
DdeI 19 CTCAG C^T[ACGT]AG
1055 CTCAG
EcoRII 850 CCTGG ^CC[AT]GG
1099 CCTGG
MflI 413 GGATCC [GA]^GATC[CT]
879 GGATCC
MvaI 850 CCTGG CC^[AT]GG
1099 CCTGG
SfcI 299 CTACAG C^T[GA][CT]AG
1049 CTGTAG
TaqI 89 TCGA T^CGA
177 TCGA
TspRI 302 CAGTG CA[GC]TG[ACGT][ACGT]^
861 CACTG
TthHB8I 89 TCGA T^CGA
177 TCGA
XhoII 413 GGATCC [GA]^GATC[CT]
879 GGATCC
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# 3 OR MORE CUT ENZYMES ( 38 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
BbvI BfaI BsaJI BsiYI BslI
BsrI BssKI BstF5I DpnII EaeI
Fnu4HI FokI HaeIII HinfI ItaI
MaeI MaeIII MboI MboII MnlI
MseI NdeII NlaIII NlaIV RsaI
Sau3AI ScrFI SfaNI TfiI Tru9I
TseI Tsp45I Tsp509I