Restriction map for
pFN21AE2228
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# NO CUT ENZYMES ( 164 enzymes) #
##################################
AatII Acc65I AclI AcyI AflII
AgeI AhdI Alw44I AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AspI
AvaI AviII AvrII BalI BamHI
BanI BanII BbeI BbsI BcgI
BciVI BclI BfrI BglII BlnI
BlpI BpmI Bpu1102I BpuAI BsaHI
BsaI BseRI BsiEI BsiHKAI BsiWI
BsmAI BsmBI BsmFI BsoBI Bsp1407I
BspDI BspHI BspLU11I BspMI BsrBI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstNI BstPI
BstXI BstYI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DraI DraII
DraIII DrdI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRI EcoRII EcoRV
EcoT22I FauI FbaI FseI FspI
HaeII HgaI Hin1I HindIII HpaI
KasI KpnI Ksp632I KspI MflI
MluI MluNI MscI MspA1I MvaI
NaeI NarI NdeI NgoMIV NheI
NlaIV NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PleI PmeI PpuMI PshAI PshBI
Psp1406I PstI PvuI PvuII RcaI
RsrII SacI SacII SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
Sse8387I SspBI SspI StuI SwaI
Tth111I Van91I XbaI XcmI XhoI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 56 enzymes) #
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AccI 326 GTCGAC GT^[AC][GT]AC
AccII 12 CGCG CG^CG
AccIII 431 TCCGGA T^CCGGA
AcsI 295 AAATTT [GA]^AATT[CT]
AfaI 437 GTAC GT^AC
AflIII 679 ACACGT A^C[GA][CT]GT
AlwI 3 GGATC GGATC(4/5)
ApoI 295 AAATTT [GA]^AATT[CT]
AvaII 54 GGACC G^G[AT]CC
BbrPI 680 CACGTG CAC^GTG
BbvCI 57 CCTCAGC CCTCAGC(-5/-2)
BbvI 8 GCAGC GCAGC(8/12)
BcnI 171 CCGGG CC^[GC]GG
BfaI 742 CTAG C^TAG
BglI 62 GCCGGAAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BmyI 683 GTGCCC G[AGT]GC[ACT]^C
BsaAI 680 CACGTG [CT]AC^GT[GA]
BsaBI 733 GATGATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 431 TCCGGA [AT]^CCGG[AT]
BseAI 431 TCCGGA T^CCGGA
BsiYI 165 CCTTGACCGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 165 CCTTGACCGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmI 354 GCATTC GAATGC(1/-1)
Bsp1286I 683 GTGCCC G[AGT]GC[ACT]^C
BspEI 431 TCCGGA T^CCGGA
BsrDI 36 GCAATG GCAATG(2/0)
BssKI 171 CCGGG ^CC[ACGT]GG
BstUI 12 CGCG CG^CG
Cfr13I 54 GGACC G^G[ACGT]CC
DsaI 359 CCATGG C^C[GA][CT]GG
EaeI 150 TGGCCG [CT]^GGCC[GA]
EcoT14I 359 CCATGG C^C[AT][AT]GG
HaeIII 151 GGCC GG^CC
MaeI 742 CTAG C^TAG
MaeII 681 ACGT A^CGT
MamI 733 GATGATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 331 CAATTG C^AATTG
MroI 431 TCCGGA T^CCGGA
MslI 393 CATAACGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 331 CAATTG C^AATTG
MvnI 12 CGCG CG^CG
NciI 171 CCGGG CC^[GC]GG
NcoI 359 CCATGG C^CATGG
PmaCI 680 CACGTG CAC^GTG
PmlI 680 CACGTG CAC^GTG
RsaI 437 GTAC GT^AC
SalI 326 GTCGAC G^TCGAC
Sau96I 54 GGACC G^G[ACGT]CC
ScrFI 171 CCGGG CC^[ACGT]GG
SfcI 574 CTACAG C^T[GA][CT]AG
SphI 579 GCATGC GCATG^C
StyI 359 CCATGG C^C[AT][AT]GG
TaqI 327 TCGA T^CGA
TseI 8 GCAGC G^C[AT]GC
Tsp45I 484 GTGAC ^GT[GC]AC
TthHB8I 327 TCGA T^CGA
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# TWO CUT ENZYMES ( 12 enzymes) #
###################################
BsaJI 171 CCGGGG C^C[ACGT][ACGT]GG
359 CCATGG
BsgI 100 GTGCAG GTGCAG(16/14)
319 GTGCAG
BsrI 219 ACTGG ACTGG(1/-1)
687 CCAGT
DdeI 58 CTCAG C^T[ACGT]AG
447 CTTAG
Fnu4HI 8 GCAGC GC^[ACGT]GC
152 GCCGC
HincII 76 GTTGAC GT[CT]^[GA]AC
326 GTCGAC
HindII 76 GTTGAC GT[CT]^[GA]AC
326 GTCGAC
ItaI 8 GCAGC GC^[ACGT]GC
152 GCCGC
MseI 460 TTAA T^TAA
511 TTAA
MwoI 62 GCCGGAAGGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
146 GCGCTGGCCGC
NspI 113 ACATGC [GA]CATG^[CT]
579 GCATGC
Tru9I 460 TTAA T^TAA
511 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 26 enzymes) #
#########################################
AciI AluI AlwNI BstF5I Cac8I
CfoI DpnII FokI HapII HhaI
HinP1I HinfI HpaII HphI MaeIII
MboI MboII MnlI MspI NdeII
NlaIII Sau3AI SfaNI TfiI Tsp509I
TspRI