Restriction map for
pFN21AE3249
##################################
# NO CUT ENZYMES ( 118 enzymes) #
##################################
AccI AccII AccIII AclI AflII
AhdI Alw44I Aor51HI ApaI ApaLI
AscI AseI AsnI AspEI AspHI
AspI AviII AvrII BamHI BbeI
BcgI BciVI BclI BcnI BfrI
BlnI BseAI BseRI BsiHKAI BsiWI
BsmBI BsmI BspDI BspEI BspMI
BsrBI BssHII BssSI Bst1107I BstAPI
BstBI BstF5I BstUI BstZ17I CfoI
ClaI CpoI DraIII DrdI EagI
Eam1105I EarI EclXI Eco47III Eco52I
EcoNI EcoRI EcoRV FbaI FokI
FseI FspI HaeII HhaI HinP1I
HindIII KasI Ksp632I KspI MfeI
MluI MroI MspA1I MunI MvnI
NaeI NarI NciI NdeI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PflFI PflMI PmeI PshAI
PshBI Psp1406I PstI PvuI PvuII
RsrII SacI SacII SalI SapI
SbfI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI Sse8387I
SspI SwaI Tth111I Van91I XbaI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AatII 855 GACGTC GACGT^C
Acc65I 960 GGTACC G^GTACC
AcsI 230 AAATTC [GA]^AATT[CT]
AgeI 1184 ACCGGT A^CCGGT
AlwNI 612 CAGTTTCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1487 CCTCAGG CC^T[ACGT]AGG
ApoI 230 AAATTC [GA]^AATT[CT]
Asp700I 881 GAACGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 960 GGTACC G^GTACC
AvaI 1554 CCCGAG C^[CT]CG[GA]G
BbrPI 255 CACGTG CAC^GTG
BbvCI 1371 CCTCAGC CCTCAGC(-5/-2)
BfaI 796 CTAG C^TAG
BglI 1321 GCCATGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1200 AGATCT A^GATCT
BlpI 1126 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1126 GCTCAGC GC^T[ACGT]AGC
BsaAI 255 CACGTG [CT]AC^GT[GA]
BsaBI 316 GATTCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI 68 GTGCAG GTGCAG(16/14)
BsiEI 758 CGGTCG CG[GA][CT]^CG
BsoBI 1554 CCCGAG C^[CT]CG[GA]G
BspHI 1640 TCATGA T^CATGA
BspLU11I 1158 ACATGT A^CATGT
BsrFI 1184 ACCGGT [GA]^CCGG[CT]
BstEII 1221 GGTCACC G^GT[ACGT]ACC
BstPI 1221 GGTCACC G^GT[ACGT]ACC
Bsu36I 1487 CCTCAGG CC^T[ACGT]AGG
CelII 1126 GCTCAGC GC^T[ACGT]AGC
Cfr10I 1184 ACCGGT [GA]^CCGG[CT]
DraI 222 TTTAAA TTT^AAA
Eco81I 1487 CCTCAGG CC^T[ACGT]AGG
EcoO65I 1221 GGTCACC G^GT[ACGT]ACC
FauI 493 GCGGG CCCGC(4/6)
HgaI 932 GCGTC GACGC(5/10)
HpaI 367 GTTAAC GTT^AAC
KpnI 960 GGTACC GGTAC^C
MaeI 796 CTAG C^TAG
MamI 316 GATTCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MslI 1419 CATGCCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
PinAI 1184 ACCGGT A^CCGGT
PmaCI 255 CACGTG CAC^GTG
PmlI 255 CACGTG CAC^GTG
RcaI 1640 TCATGA T^CATGA
ScaI 1348 AGTACT AGT^ACT
SfcI 1561 CTACAG C^T[GA][CT]AG
SmlI 468 CTTGAG C^T[CT][GA]AG
StuI 1368 AGGCCT AGG^CCT
XmnI 881 GAACGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AcyI 855 GACGTC G[GA]^CG[CT]C
931 GGCGTC
AflIII 256 ACGTGT A^C[GA][CT]GT
1158 ACATGT
BalI 1165 TGGCCA TGG^CCA
1274 TGGCCA
BanI 960 GGTACC G^G[CT][GA]CC
1477 GGTGCC
BanII 133 GAGCCC G[GA]GC[CT]^C
709 GGGCTC
BbsI 637 GAAGAC GAAGAC(2/6)
885 GTCTTC
BpmI 400 CTCCAG CTGGAG(16/14)
689 CTGGAG
BpuAI 637 GAAGAC GAAGAC(2/6)
885 GTCTTC
BsaHI 855 GACGTC G[GA]^CG[CT]C
931 GGCGTC
BsaWI 1184 ACCGGT [AT]^CCGG[AT]
1225 ACCGGA
Bsp1407I 207 TGTACA T^GTACA
1506 TGTACA
BsrDI 1290 CATTGC GCAATG(2/0)
1516 GCAATG
BsrGI 207 TGTACA T^GTACA
1506 TGTACA
BstXI 928 CCAGGCGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1289 CCATTGCTTTGG
BstYI 1110 GGATCT [GA]^GATC[CT]
1200 AGATCT
EaeI 1165 TGGCCA [CT]^GGCC[GA]
1274 TGGCCA
EcoT22I 1088 ATGCAT ATGCA^T
1399 ATGCAT
HapII 1185 CCGG C^CGG
1226 CCGG
Hin1I 855 GACGTC G[GA]^CG[CT]C
931 GGCGTC
HincII 301 GTCAAC GT[CT]^[GA]AC
367 GTTAAC
HindII 301 GTCAAC GT[CT]^[GA]AC
367 GTTAAC
HpaII 1185 CCGG C^CGG
1226 CCGG
MboII 637 GAAGA GAAGA(8/7)
886 TCTTC
MflI 1110 GGATCT [GA]^GATC[CT]
1200 AGATCT
MluNI 1165 TGGCCA TGG^CCA
1274 TGGCCA
MscI 1165 TGGCCA TGG^CCA
1274 TGGCCA
MspI 1185 CCGG C^CGG
1226 CCGG
MwoI 757 GCGGTCGCAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
1321 GCCATGGAGGC
NsiI 1088 ATGCAT ATGCA^T
1399 ATGCAT
PleI 1302 GACTC GAGTC(4/5)
1447 GAGTC
PpuMI 92 GGGTCCT [GA]G^G[AT]CC[CT]
1244 AGGACCT
SphI 1401 GCATGC GCATG^C
1418 GCATGC
SspBI 207 TGTACA T^GTACA
1506 TGTACA
TfiI 316 GATTC G^A[AT]TC
1380 GAATC
XhoII 1110 GGATCT [GA]^GATC[CT]
1200 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 56 enzymes) #
#########################################
AciI AfaI AluI AlwI AvaII
BbvI BmyI BsaI BsaJI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrI
BssKI BstNI Cac8I Cfr13I DdeI
DpnII DraII DsaI EcoO109I EcoRII
EcoT14I Fnu4HI HaeIII HinfI HphI
ItaI MaeII MaeIII MboI MnlI
MseI MvaI NcoI NdeII NlaIII
NlaIV NspI RsaI Sau3AI Sau96I
ScrFI SfaNI StyI TaqI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XcmI