Restriction map for
pFN21AE5664
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# NO CUT ENZYMES ( 152 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AflII AflIII
AgeI AhdI Alw44I Aor51HI ApaI
ApaLI AscI Asp718I AspEI AspHI
AspI AvaII AviII BamHI BanI
BanII BbeI BbrPI BbvCI BcgI
BclI BcnI BfrI BglII BlpI
BmyI Bpu1102I BsaAI BsaHI BsaI
BseAI BseRI BsiEI BsiHKAI BsiWI
BsmAI BsmBI Bsp1286I Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstXI BstZ17I CelII CfoI
Cfr10I ClaI CpoI DraII DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoO109I EcoO65I EcoRI
EcoRV EcoT22I FauI FbaI FseI
FspI HaeII HgaI HhaI Hin1I
HinP1I HindIII KasI KpnI KspI
MaeII MluI MroI MvnI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PflFI PflMI PinAI PleI
PmaCI PmeI PmlI PpuMI PshAI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SapI SbfI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I SspBI SspI
SwaI Tth111I Van91I XbaI XcmI
XmaCI XmaI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AlwI 836 GGATC GGATC(4/5)
AlwNI 151 CAGGGACTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1360 CCTCAGG CC^T[ACGT]AGG
AseI 883 ATTAAT AT^TAAT
AsnI 883 ATTAAT AT^TAAT
Asp700I 579 GAAGCTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 1088 CTCGAG C^[CT]CG[GA]G
AvrII 1047 CCTAGG C^CTAGG
BalI 1281 TGGCCA TGG^CCA
BbsI 1471 GAAGAC GAAGAC(2/6)
BglI 1274 GCCTGCATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 1047 CCTAGG C^CTAGG
BpmI 72 CTGGAG CTGGAG(16/14)
BpuAI 1471 GAAGAC GAAGAC(2/6)
BsaBI 949 GATATTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 435 TCCGGT [AT]^CCGG[AT]
BsgI 1220 GTGCAG GTGCAG(16/14)
BsmI 814 GAATGC GAATGC(1/-1)
BsoBI 1088 CTCGAG C^[CT]CG[GA]G
BstYI 836 GGATCT [GA]^GATC[CT]
Bsu36I 1360 CCTCAGG CC^T[ACGT]AGG
DsaI 418 CCACGG C^C[GA][CT]GG
EaeI 1281 TGGCCA [CT]^GGCC[GA]
EarI 1508 CTCTTC CTCTTC(1/4)
Eco81I 1360 CCTCAGG CC^T[ACGT]AGG
HapII 436 CCGG C^CGG
HincII 127 GTTAAC GT[CT]^[GA]AC
HindII 127 GTTAAC GT[CT]^[GA]AC
HpaI 127 GTTAAC GTT^AAC
HpaII 436 CCGG C^CGG
HphI 636 TCACC GGTGA(8/7)
Ksp632I 1508 CTCTTC CTCTTC(1/4)
MamI 949 GATATTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 849 CAATTG C^AATTG
MflI 836 GGATCT [GA]^GATC[CT]
MluNI 1281 TGGCCA TGG^CCA
MscI 1281 TGGCCA TGG^CCA
MslI 1103 CACTTTTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 319 CAGCTG C[AC]G^C[GT]G
MspI 436 CCGG C^CGG
MunI 849 CAATTG C^AATTG
NlaIV 438 GGTTCC GG[ACGT]^[ACGT]CC
PaeR7I 1088 CTCGAG C^TCGAG
PshBI 883 ATTAAT AT^TAAT
PstI 1010 CTGCAG CTGCA^G
PvuII 319 CAGCTG CAG^CTG
SfcI 1010 CTGCAG C^T[GA][CT]AG
SphI 1005 GCATGC GCATG^C
StuI 240 AGGCCT AGG^CCT
Tsp45I 173 GTGAC ^GT[GC]AC
XhoI 1088 CTCGAG C^TCGAG
XhoII 836 GGATCT [GA]^GATC[CT]
XmnI 579 GAAGCTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 13 enzymes) #
###################################
BciVI 1118 GGATAC GTATCC(6/5)
1503 GGATAC
BsmFI 153 GGGAC GGGAC(10/14)
346 GTCCC
Cfr13I 975 GGGCC G^G[ACGT]CC
1076 GGGCC
DpnII 837 GATC ^GATC
846 GATC
DraI 742 TTTAAA TTT^AAA
933 TTTAAA
EcoT14I 588 CCTTGG C^C[AT][AT]GG
1047 CCTAGG
MboI 837 GATC ^GATC
846 GATC
NdeII 837 GATC ^GATC
846 GATC
NspI 990 ACATGC [GA]CATG^[CT]
1005 GCATGC
Sau3AI 837 GATC ^GATC
846 GATC
Sau96I 975 GGGCC G^G[ACGT]CC
1076 GGGCC
SmlI 246 CTCAAG C^T[CT][GA]AG
1088 CTCGAG
StyI 588 CCTTGG C^C[AT][AT]GG
1047 CCTAGG
#########################################
# 3 OR MORE CUT ENZYMES ( 40 enzymes) #
#########################################
AcsI AfaI AluI ApoI BbvI
BfaI BsaJI BsiYI BslI BsrI
BssKI BstF5I BstNI Cac8I DdeI
EcoRII Fnu4HI FokI HaeIII HinfI
ItaI MaeI MaeIII MboII MnlI
MseI MvaI MwoI NlaIII RsaI
ScaI ScrFI SfaNI TaqI TfiI
Tru9I TseI Tsp509I TspRI TthHB8I