Restriction map for
pFN21AE5586
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# NO CUT ENZYMES ( 125 enzymes) #
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AatII Acc65I AccIII AclI AcsI
AflII AflIII AgeI AhdI Alw44I
AocI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AvrII BalI BbeI BcgI BciVI
BclI BfrI BglII BlnI BsaBI
BsaI BsaWI BseAI BseRI BsiEI
BsiWI BsmAI BsmBI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrDI BsrGI BstAPI BstBI BstEII
BstPI BstXI Bsu36I ClaI CpoI
DraI DraII DrdI EagI Eam1105I
EclXI Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI HincII HindII HindIII HpaI
KasI KpnI MamI MfeI MluI
MluNI MroI MscI MseI MslI
MunI NarI NcoI NdeI NlaIII
NotI NruI NsiI NspI NspV
PacI PaeR7I PinAI PmeI PpuMI
PshAI PshBI Psp1406I PvuI PvuII
RcaI RsrII SalI SapI ScaI
SfiI SfoI SfuI SmlI SnaBI
SpeI SphI SspBI SspI SwaI
Tru9I Tsp509I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AccI 783 GTATAC GT^[AC][GT]AC
AcyI 259 GACGCC G[GA]^CG[CT]C
Aor51HI 288 AGCGCT AGC^GCT
AspHI 1064 GAGCTC G[AT]GC[AT]^C
AspI 151 GACGCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 140 TGCGCA TGC^GCA
BanI 340 GGTGCC G^G[CT][GA]CC
BbsI 973 GTCTTC GAAGAC(2/6)
BbvCI 381 CCTCAGC CCTCAGC(-5/-2)
BlpI 570 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 570 GCTGAGC GC^T[ACGT]AGC
BpuAI 973 GTCTTC GAAGAC(2/6)
BsaHI 259 GACGCC G[GA]^CG[CT]C
BsgI 501 CTGCAC GTGCAG(16/14)
BsiHKAI 1064 GAGCTC G[AT]GC[AT]^C
BspMI 71 ACCTGC ACCTGC(4/8)
BssHII 402 GCGCGC G^CGCGC
BssSI 904 CACGAG CACGAG(-5/-1)
Bst1107I 783 GTATAC GTA^TAC
BstF5I 378 CATCC GGATG(2/0)
BstZ17I 783 GTATAC GTA^TAC
CelII 570 GCTGAGC GC^T[ACGT]AGC
DraIII 217 CACGCCGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 2 TGGCCG [CT]^GGCC[GA]
EarI 82 CTCTTC CTCTTC(1/4)
Eco47III 288 AGCGCT AGC^GCT
EcoT14I 494 CCTTGG C^C[AT][AT]GG
FokI 378 CATCC GGATG(9/13)
FspI 140 TGCGCA TGC^GCA
Hin1I 259 GACGCC G[GA]^CG[CT]C
Ksp632I 82 CTCTTC CTCTTC(1/4)
KspI 414 CCGCGG CCGC^GG
MaeIII 215 GTCAC ^GT[ACGT]AC
NheI 132 GCTAGC G^CTAGC
PflFI 151 GACGCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 694 GAGTC GAGTC(4/5)
SacI 1064 GAGCTC GAGCT^C
SacII 414 CCGCGG CCGC^GG
SbfI 648 CCTGCAGG CCTGCA^GG
SexAI 725 ACCAGGT A^CC[AT]GGT
SfaNI 377 GCATC GCATC(5/9)
SgrAI 261 CGCCGGCG C[GA]^CCGG[CT]G
Sse8387I 648 CCTGCAGG CCTGCA^GG
StuI 762 AGGCCT AGG^CCT
StyI 494 CCTTGG C^C[AT][AT]GG
TfiI 1020 GATTC G^A[AT]TC
Tsp45I 215 GTCAC ^GT[GC]AC
Tth111I 151 GACGCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
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# TWO CUT ENZYMES ( 20 enzymes) #
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AfaI 198 GTAC GT^AC
1081 GTAC
BanII 573 GAGCCC G[GA]GC[CT]^C
1064 GAGCTC
BbrPI 40 CACGTG CAC^GTG
772 CACGTG
BsaAI 40 CACGTG [CT]AC^GT[GA]
772 CACGTG
BsrFI 262 GCCGGC [GA]^CCGG[CT]
373 GCCGGC
BsrI 1039 ACTGG ACTGG(1/-1)
834 CCAGT
Cfr10I 262 GCCGGC [GA]^CCGG[CT]
373 GCCGGC
DsaI 356 CCGTGG C^C[GA][CT]GG
414 CCGCGG
HinfI 694 GAGTC G^A[ACGT]TC
1020 GATTC
NaeI 262 GCCGGC GCC^GGC
373 GCCGGC
NgoMIV 262 GCCGGC G^CCGGC
373 GCCGGC
PflMI 93 CCAGCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
834 CCAGTTCCTGG
PmaCI 40 CACGTG CAC^GTG
772 CACGTG
PmlI 40 CACGTG CAC^GTG
772 CACGTG
RsaI 198 GTAC GT^AC
1081 GTAC
SmaI 275 CCCGGG CCC^GGG
463 CCCGGG
TspRI 807 CACTG CA[GC]TG[ACGT][ACGT]^
1038 CACTG
Van91I 93 CCAGCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
834 CCAGTTCCTGG
XmaCI 275 CCCGGG C^CCGGG
463 CCCGGG
XmaI 275 CCCGGG C^CCGGG
463 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 65 enzymes) #
#########################################
AccII AciI AluI AlwI AlwNI
AvaI AvaII BamHI BbvI BcnI
BfaI BglI BmyI BpmI BsaJI
BsiYI BslI BsmFI BsoBI Bsp1286I
BssKI BstNI BstUI BstYI Cac8I
CfoI Cfr13I DdeI DpnII EcoRII
FauI Fnu4HI HaeII HaeIII HapII
HgaI HhaI HinP1I HpaII HphI
ItaI MaeI MaeII MboI MboII
MflI MnlI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIV
PstI Sau3AI Sau96I ScrFI SfcI
TaqI TseI TthHB8I XcmI XhoII