Restriction map for
pFN21AE6275
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# NO CUT ENZYMES ( 167 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AcyI AfaI AflII AflIII
AgeI AhdI Alw44I AlwNI AocI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AvaI AviII AvrII
BamHI BanI BanII BbeI BbrPI
BbvCI BcgI BciVI BclI BfrI
BlnI BmyI BsaAI BsaHI BsaI
BsaWI BseAI BsgI BsiEI BsiHKAI
BsiWI BsmBI BsmFI BsmI BsoBI
Bsp1286I Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrDI BsrGI
BsrI BssSI Bst1107I BstAPI BstBI
BstEII BstF5I BstPI BstZ17I Bsu36I
ClaI CpoI DraI DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FauI FbaI FokI FseI
FspI HgaI Hin1I HincII HindII
HindIII HpaI KasI KpnI KspI
MaeII MluI MroI MseI MspA1I
NaeI NarI NcoI NdeI NgoMIV
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI PvuII
RcaI RsaI RsrII SacI SacII
SalI SapI SbfI ScaI SfcI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI TaqI Tru9I Tth111I
TthHB8I Van91I XbaI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AccII 54 CGCG CG^CG
AciI 99 CCGC CCGC(-3/-1)
AluI 168 AGCT AG^CT
AlwI 254 GGATC GGATC(4/5)
AvaII 146 GGTCC G^G[AT]CC
BalI 299 TGGCCA TGG^CCA
BbsI 31 GTCTTC GAAGAC(2/6)
BcnI 26 CCGGG CC^[GC]GG
BglI 40 GCCTTCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 539 AGATCT A^GATCT
BlpI 343 GCTGAGC GC^T[ACGT]AGC
BpmI 594 CTCCAG CTGGAG(16/14)
Bpu1102I 343 GCTGAGC GC^T[ACGT]AGC
BpuAI 31 GTCTTC GAAGAC(2/6)
BsaBI 525 GATTCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmAI 127 GAGAC GTCTC(1/5)
BsrFI 214 ACCGGC [GA]^CCGG[CT]
BssHII 53 GCGCGC G^CGCGC
BstUI 54 CGCG CG^CG
BstXI 263 CCAAGAACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 539 AGATCT [GA]^GATC[CT]
CelII 343 GCTGAGC GC^T[ACGT]AGC
Cfr10I 214 ACCGGC [GA]^CCGG[CT]
DraII 245 AGGCCCC [GA]G^G[ACGT]CC[CT]
DsaI 62 CCGTGG C^C[GA][CT]GG
EarI 445 CTCTTC CTCTTC(1/4)
EcoO109I 245 AGGCCCC [GA]G^G[ACGT]CC[CT]
EcoT14I 368 CCAAGG C^C[AT][AT]GG
HaeII 550 AGCGCC [GA]GCGC^[CT]
Ksp632I 445 CTCTTC CTCTTC(1/4)
MaeIII 611 GTGAC ^GT[ACGT]AC
MamI 525 GATTCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 323 CAATTG C^AATTG
MflI 539 AGATCT [GA]^GATC[CT]
MluNI 299 TGGCCA TGG^CCA
MscI 299 TGGCCA TGG^CCA
MslI 312 CATCATCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 323 CAATTG C^AATTG
MvnI 54 CGCG CG^CG
NciI 26 CCGGG CC^[GC]GG
NheI 9 GCTAGC G^CTAGC
SexAI 269 ACCTGGT A^CC[AT]GGT
SfiI 39 GGCCTTCTGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
StuI 432 AGGCCT AGG^CCT
StyI 368 CCAAGG C^C[AT][AT]GG
TfiI 525 GATTC G^A[AT]TC
Tsp45I 611 GTGAC ^GT[GC]AC
XhoII 539 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 15 enzymes) #
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BfaI 10 CTAG C^TAG
166 CTAG
BseRI 219 CTCCTC GAGGAG(10/8)
236 GAGGAG
DdeI 344 CTGAG C^T[ACGT]AG
500 CTCAG
DpnII 255 GATC ^GATC
540 GATC
HapII 26 CCGG C^CGG
215 CCGG
HpaII 26 CCGG C^CGG
215 CCGG
HphI 610 GGTGA GGTGA(8/7)
425 TCACC
MaeI 10 CTAG C^TAG
166 CTAG
MboI 255 GATC ^GATC
540 GATC
MspI 26 CCGG C^CGG
215 CCGG
NdeII 255 GATC ^GATC
540 GATC
PleI 68 GAGTC GAGTC(4/5)
455 GAGTC
Sau3AI 255 GATC ^GATC
540 GATC
Tsp509I 324 AATT ^AATT
582 AATT
XcmI 80 CCATTTTTGATTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
596 CCAGAGTGAAGATGG
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# 3 OR MORE CUT ENZYMES ( 28 enzymes) #
#########################################
BbvI BsaJI BsiYI BslI BssKI
BstNI Cac8I CfoI Cfr13I EaeI
EcoRII Fnu4HI HaeIII HhaI HinP1I
HinfI ItaI MboII MnlI MvaI
MwoI NlaIII NlaIV Sau96I ScrFI
SfaNI TseI TspRI