Restriction map for
pFN21AE1936
##################################
# NO CUT ENZYMES ( 117 enzymes) #
##################################
AatII AccI AccII AclI AcsI
AcyI AflII AgeI Alw44I AlwNI
AocI ApaI ApaLI ApoI AscI
AseI AsnI AviII BalI BamHI
BbeI BcgI BciVI BclI BfrI
BglI BlpI Bpu1102I BsaHI BsaI
BseRI BsgI BsiEI BsiWI BsmBI
BsmI Bsp1407I BspDI BsrBI BsrDI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstUI BstZ17I Bsu36I
CelII ClaI CpoI DraI EaeI
EagI EclXI Eco52I Eco81I EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HgaI Hin1I HincII HindII
HpaI KasI KspI MluI MluNI
MscI MspA1I MvnI NarI NcoI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PflMI PinAI
PmeI PpuMI PshAI PshBI Psp1406I
PvuI PvuII RsrII SacII SalI
SexAI SfiI SfoI SfuI SgrAI
SnaBI SpeI SphI SspBI SspI
StuI SwaI TaqI TthHB8I Van91I
XbaI XhoI
###################################
# ONE CUT ENZYMES ( 67 enzymes) #
###################################
Acc65I 174 GGTACC G^GTACC
AccIII 644 TCCGGA T^CCGGA
AflIII 567 ACATGT A^C[GA][CT]GT
AhdI 358 GACCAAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 215 AGCGCT AGC^GCT
Asp700I 550 GAAAAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 174 GGTACC G^GTACC
AspEI 358 GACCAAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 1405 GAGCTC G[AT]GC[AT]^C
AspI 995 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 166 CCCGGG C^[CT]CG[GA]G
AvrII 846 CCTAGG C^CTAGG
BanI 174 GGTACC G^G[CT][GA]CC
BbrPI 112 CACGTG CAC^GTG
BbsI 55 GTCTTC GAAGAC(2/6)
BbvCI 458 CCTCAGC CCTCAGC(-5/-2)
BglII 264 AGATCT A^GATCT
BlnI 846 CCTAGG C^CTAGG
BpmI 687 CTGGAG CTGGAG(16/14)
BpuAI 55 GTCTTC GAAGAC(2/6)
BsaAI 112 CACGTG [CT]AC^GT[GA]
BsaBI 259 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 644 TCCGGA [AT]^CCGG[AT]
BseAI 644 TCCGGA T^CCGGA
BsiHKAI 1405 GAGCTC G[AT]GC[AT]^C
BsoBI 166 CCCGGG C^[CT]CG[GA]G
BspEI 644 TCCGGA T^CCGGA
BspHI 1197 TCATGA T^CATGA
BspLU11I 567 ACATGT A^CATGT
BsrFI 100 GCCGGC [GA]^CCGG[CT]
BstAPI 91 GCAGGGGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 1290 CCATGCTAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 100 GCCGGC [GA]^CCGG[CT]
DraII 1343 AGGCCCC [GA]G^G[ACGT]CC[CT]
DraIII 1064 CACGGTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1122 GACCAACAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 1063 CCACGG C^C[GA][CT]GG
Eam1105I 358 GACCAAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 215 AGCGCT AGC^GCT
EcoNI 756 CCTTTCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 1343 AGGCCCC [GA]G^G[ACGT]CC[CT]
HaeII 215 AGCGCT [GA]GCGC^[CT]
HindIII 553 AAGCTT A^AGCTT
KpnI 174 GGTACC GGTAC^C
MamI 259 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1262 CAATTG C^AATTG
MroI 644 TCCGGA T^CCGGA
MseI 1052 TTAA T^TAA
MunI 1262 CAATTG C^AATTG
NaeI 100 GCCGGC GCC^GGC
NgoMIV 100 GCCGGC G^CCGGC
NspI 567 ACATGT [GA]CATG^[CT]
PflFI 995 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 112 CACGTG CAC^GTG
PmlI 112 CACGTG CAC^GTG
RcaI 1197 TCATGA T^CATGA
SacI 1405 GAGCTC GAGCT^C
SbfI 420 CCTGCAGG CCTGCA^GG
ScaI 857 AGTACT AGT^ACT
SmaI 166 CCCGGG CCC^GGG
Sse8387I 420 CCTGCAGG CCTGCA^GG
Tru9I 1052 TTAA T^TAA
Tth111I 995 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 1152 CCACAATTTCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 166 CCCGGG C^CCGGG
XmaI 166 CCCGGG C^CCGGG
XmnI 550 GAAAAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 15 enzymes) #
###################################
BanII 695 GAGCCC G[GA]GC[CT]^C
1405 GAGCTC
BspMI 969 ACCTGC ACCTGC(4/8)
423 GCAGGT
BstYI 264 AGATCT [GA]^GATC[CT]
335 AGATCC
Cac8I 19 GCTGGC GC[ACGT]^[ACGT]GC
100 GCCGGC
CfoI 216 GCGC GCG^C
348 GCGC
FauI 210 CCCGC CCCGC(4/6)
1032 CCCGC
HhaI 216 GCGC GCG^C
348 GCGC
HinP1I 216 GCGC G^CGC
348 GCGC
MaeII 113 ACGT A^CGT
344 ACGT
MflI 264 AGATCT [GA]^GATC[CT]
335 AGATCC
MslI 504 CATCATGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1291 CATGCTAATG
SapI 539 GAAGAGC GCTCTTC(1/4)
692 GAAGAGC
TfiI 915 GATTC G^A[AT]TC
1258 GAATC
Tsp509I 1156 AATT ^AATT
1263 AATT
XhoII 264 AGATCT [GA]^GATC[CT]
335 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 59 enzymes) #
#########################################
AciI AfaI AluI AlwI AvaII
BbvI BcnI BfaI BmyI BsaJI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI Cfr13I
DdeI DpnII EarI EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeIII MboI MboII MnlI MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV PleI PstI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TseI Tsp45I TspRI