Restriction map for
pFN21AE3041
##################################
# NO CUT ENZYMES ( 171 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcsI AfaI AflII AgeI AhdI
Alw44I AlwI AocI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvaI
AviII AvrII BalI BamHI BanII
BbrPI BbsI BcgI BciVI BclI
BfrI BglI BglII BlnI BlpI
Bpu1102I BpuAI BsaAI BsaBI BsaWI
BseAI BseRI BsgI BsiEI BsiWI
BsmBI BsmI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrDI
BsrGI BssHII Bst1107I BstAPI BstEII
BstF5I BstPI BstUI BstXI BstYI
BstZ17I Bsu36I CelII ClaI CpoI
DpnII DraI DrdI DsaI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FbaI FokI FseI FspI
HincII HindII HindIII HinfI HpaI
KpnI KspI MaeIII MamI MboI
MfeI MflI MluI MluNI MroI
MscI MseI MspA1I MunI MvnI
NcoI NdeI NdeII NotI NruI
NsiI NspI PacI PaeR7I PflFI
PflMI PinAI PleI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
PvuII RcaI RsaI RsrII SacI
SacII SalI SapI Sau3AI SbfI
ScaI SfaNI SfiI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI TfiI Tru9I
Tsp45I Tsp509I Tth111I Van91I XbaI
XcmI XhoI XhoII XmaCI XmaI
XmnI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
AccI 178 GTCTAC GT^[AC][GT]AC
AflIII 386 ACGTGT A^C[GA][CT]GT
AlwNI 153 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG
BbeI 263 GGCGCC GGCGC^C
BbvCI 438 GCTGAGG CCTCAGC(-5/-2)
BcnI 353 CCGGG CC^[GC]GG
BfaI 37 CTAG C^TAG
BsaI 3 GGTCTC GGTCTC(1/5)
BsmAI 4 GTCTC GTCTC(1/5)
BsrBI 267 CCGCTC CCGCTC(-3/-3)
BsrFI 260 GCCGGC [GA]^CCGG[CT]
BssSI 46 CTCGTG CACGAG(-5/-1)
BstBI 332 TTCGAA TT^CGAA
Cfr10I 260 GCCGGC [GA]^CCGG[CT]
DdeI 439 CTGAG C^T[ACGT]AG
DraIII 18 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 528 GAAGAG CTCTTC(1/4)
HphI 207 GGTGA GGTGA(8/7)
KasI 263 GGCGCC G^GCGCC
Ksp632I 528 GAAGAG CTCTTC(1/4)
MaeI 37 CTAG C^TAG
MaeII 386 ACGT A^CGT
MboII 528 GAAGA GAAGA(8/7)
MslI 201 CATCTGGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 260 GCCGGC GCC^GGC
NarI 263 GGCGCC GG^CGCC
NciI 353 CCGGG CC^[GC]GG
NgoMIV 260 GCCGGC G^CCGGC
NheI 36 GCTAGC G^CTAGC
NspV 332 TTCGAA TT^CGAA
PpuMI 305 GGGACCC [GA]G^G[AT]CC[CT]
SexAI 19 ACCTGGT A^CC[AT]GGT
SfoI 263 GGCGCC GGC^GCC
SfuI 332 TTCGAA TT^CGAA
SgrAI 259 CGCCGGCG C[GA]^CCGG[CT]G
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AcyI 257 GACGCC G[GA]^CG[CT]C
263 GGCGCC
AspHI 24 GTGCTC G[AT]GC[AT]^C
291 GTGCTC
AvaII 306 GGACC G^G[AT]CC
506 GGTCC
BanI 121 GGCACC G^G[CT][GA]CC
263 GGCGCC
BmyI 24 GTGCTC G[AGT]GC[ACT]^C
291 GTGCTC
BpmI 294 CTCCAG CTGGAG(16/14)
88 CTGGAG
BsaHI 257 GACGCC G[GA]^CG[CT]C
263 GGCGCC
BsiHKAI 24 GTGCTC G[AT]GC[AT]^C
291 GTGCTC
Bsp1286I 24 GTGCTC G[AGT]GC[ACT]^C
291 GTGCTC
BsrI 397 ACTGG ACTGG(1/-1)
296 CCAGT
DraII 305 GGGACCC [GA]G^G[ACGT]CC[CT]
337 AGGCCCT
EcoO109I 305 GGGACCC [GA]G^G[ACGT]CC[CT]
337 AGGCCCT
EcoT14I 117 CCAAGG C^C[AT][AT]GG
277 CCAAGG
HaeII 263 GGCGCC [GA]GCGC^[CT]
281 GGCGCT
Hin1I 257 GACGCC G[GA]^CG[CT]C
263 GGCGCC
PstI 150 CTGCAG CTGCA^G
380 CTGCAG
StyI 117 CCAAGG C^C[AT][AT]GG
277 CCAAGG
TaqI 164 TCGA T^CGA
333 TCGA
TspRI 289 CAGTG CA[GC]TG[ACGT][ACGT]^
297 CAGTG
TthHB8I 164 TCGA T^CGA
333 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 32 enzymes) #
#########################################
AciI AluI BbvI BsaJI BsiYI
BslI BsmFI BssKI BstNI Cac8I
CfoI Cfr13I EcoRII FauI Fnu4HI
HaeIII HapII HgaI HhaI HinP1I
HpaII ItaI MnlI MspI MvaI
MwoI NlaIII NlaIV Sau96I ScrFI
SfcI TseI