Restriction map for
pFN21AE4306
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# NO CUT ENZYMES ( 131 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp718I AspEI AspI
AviII AvrII BbrPI BbvCI BcgI
BciVI BglI BglII BlnI BlpI
BpmI Bpu1102I BsaAI BsaWI BseAI
BseRI BsgI BsiEI BsiWI BsmBI
BspDI BspEI BspHI BspLU11I BsrDI
BssHII Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstZ17I Bsu36I
CelII ClaI CpoI DraI DraII
DraIII DrdI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoO109I
EcoO65I EcoRI EcoRV EcoT22I FseI
FspI HincII HindII HindIII HpaI
KpnI Ksp632I KspI MfeI MluI
MroI MunI MvnI NdeI NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SapI SbfI ScaI SexAI
SfiI SfuI SmaI SnaBI SpeI
SphI Sse8387I SspI StuI SwaI
Tth111I XbaI XcmI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 58 enzymes) #
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AflII 144 CTTAAG C^TTAAG
AlwNI 73 CAGCATCTG CAG[ACGT][ACGT][ACGT]^CTG
AspHI 465 GAGCTC G[AT]GC[AT]^C
AvaI 62 CTCGGG C^[CT]CG[GA]G
BalI 747 TGGCCA TGG^CCA
BamHI 303 GGATCC G^GATCC
BanI 90 GGCGCC G^G[CT][GA]CC
BanII 465 GAGCTC G[GA]GC[CT]^C
BbeI 90 GGCGCC GGCGC^C
BbsI 219 GAAGAC GAAGAC(2/6)
BclI 398 TGATCA T^GATCA
BcnI 10 CCCGG CC^[GC]GG
BfaI 838 CTAG C^TAG
BfrI 144 CTTAAG C^TTAAG
BmyI 465 GAGCTC G[AGT]GC[ACT]^C
BpuAI 219 GAAGAC GAAGAC(2/6)
BsaI 778 GAGACC GGTCTC(1/5)
BsiHKAI 465 GAGCTC G[AT]GC[AT]^C
BsmI 501 GAATGC GAATGC(1/-1)
BsoBI 62 CTCGGG C^[CT]CG[GA]G
Bsp1286I 465 GAGCTC G[AGT]GC[ACT]^C
Bsp1407I 788 TGTACA T^GTACA
BspMI 52 ACCTGC ACCTGC(4/8)
BsrBI 622 GAGCGG CCGCTC(-3/-3)
BsrFI 87 GCCGGC [GA]^CCGG[CT]
BsrGI 788 TGTACA T^GTACA
BsrI 554 ACTGG ACTGG(1/-1)
BssSI 538 CTCGTG CACGAG(-5/-1)
BstYI 303 GGATCC [GA]^GATC[CT]
Cfr10I 87 GCCGGC [GA]^CCGG[CT]
DsaI 46 CCATGG C^C[GA][CT]GG
EaeI 747 TGGCCA [CT]^GGCC[GA]
EcoNI 225 CCTGATGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 398 TGATCA T^GATCA
HgaI 4 GCGTC GACGC(5/10)
KasI 90 GGCGCC G^GCGCC
MaeI 838 CTAG C^TAG
MflI 303 GGATCC [GA]^GATC[CT]
MluNI 747 TGGCCA TGG^CCA
MscI 747 TGGCCA TGG^CCA
NaeI 87 GCCGGC GCC^GGC
NarI 90 GGCGCC GG^CGCC
NciI 10 CCCGG CC^[GC]GG
NcoI 46 CCATGG C^CATGG
NgoMIV 87 GCCGGC G^CCGGC
PflMI 907 CCATTCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 70 CTGCAG CTGCA^G
PvuII 129 CAGCTG CAG^CTG
SacI 465 GAGCTC GAGCT^C
SfoI 90 GGCGCC GGC^GCC
SgrAI 86 CGCCGGCG C[GA]^CCGG[CT]G
SmlI 144 CTTAAG C^T[CT][GA]AG
SspBI 788 TGTACA T^GTACA
TaqI 450 TCGA T^CGA
Tsp45I 479 GTGAC ^GT[GC]AC
TthHB8I 450 TCGA T^CGA
Van91I 907 CCATTCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 303 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AcsI 191 GAATTT [GA]^AATT[CT]
685 AAATTC
AcyI 3 GGCGTC G[GA]^CG[CT]C
90 GGCGCC
AfaI 513 GTAC GT^AC
789 GTAC
AlwI 304 GATCC GGATC(4/5)
303 GGATC
ApoI 191 GAATTT [GA]^AATT[CT]
685 AAATTC
Asp700I 243 GAACAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
760 GAAACCATTC
BsaBI 577 GATGTGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
610 GATATAAATC
BsaHI 3 GGCGTC G[GA]^CG[CT]C
90 GGCGCC
BsmAI 125 GAGAC GTCTC(1/5)
778 GAGAC
BsmFI 420 GGGAC GGGAC(10/14)
860 GGGAC
Cac8I 87 GCCGGC GC[ACGT]^[ACGT]GC
543 GCAAGC
FauI 84 CCCGC CCCGC(4/6)
624 GCGGG
HaeII 66 GGCGCT [GA]GCGC^[CT]
90 GGCGCC
HapII 11 CCGG C^CGG
88 CCGG
Hin1I 3 GGCGTC G[GA]^CG[CT]C
90 GGCGCC
HpaII 11 CCGG C^CGG
88 CCGG
MaeII 197 ACGT A^CGT
236 ACGT
MaeIII 274 GTAAC ^GT[ACGT]AC
479 GTGAC
MamI 577 GATGTGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
610 GATATAAATC
MseI 145 TTAA T^TAA
161 TTAA
MslI 427 CATCAAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
472 CACATCTGTG
MspA1I 94 CCGCTG C[AC]G^C[GT]G
129 CAGCTG
MspI 11 CCGG C^CGG
88 CCGG
PleI 443 GACTC GAGTC(4/5)
637 GAGTC
RsaI 513 GTAC GT^AC
789 GTAC
Tru9I 145 TTAA T^TAA
161 TTAA
XmnI 243 GAACAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
760 GAAACCATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 42 enzymes) #
#########################################
AciI AluI AvaII BbvI BsaJI
BsiYI BslI BssKI BstF5I BstNI
CfoI Cfr13I DdeI DpnII EcoRII
EcoT14I Fnu4HI FokI HaeIII HhaI
HinP1I HinfI HphI ItaI MboI
MboII MnlI MvaI MwoI NdeII
NlaIII NlaIV Sau3AI Sau96I ScrFI
SfaNI SfcI StyI TfiI TseI
Tsp509I TspRI