##################################
# NO CUT ENZYMES (  203 enzymes) #
##################################

AatII       AccI        AccII       AccIII      AclI        
AcsI        AcyI        AflII       AflIII      AgeI        
AhdI        AlwI        AlwNI       AocI        Aor51HI     
ApaI        ApoI        AscI        AseI        AsnI        
AspEI       AspI        AvaII       AviII       AvrII       
BalI        BamHI       BanII       BbeI        BbrPI       
BbsI        BbvCI       BcgI        BciVI       BclI        
BcnI        BfaI        BfrI        BglI        BglII       
BlnI        BlpI        BpmI        Bpu1102I    BpuAI       
BsaAI       BsaBI       BsaHI       BsaI        BsaWI       
BseAI       BseRI       BsiEI       BsiWI       BsiYI       
BslI        BsmFI       BsmI        Bsp1407I    BspEI       
BspHI       BspLU11I    BsrBI       BsrDI       BsrFI       
BsrGI       BsrI        BssHII      BssKI       BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstNI       
BstPI       BstUI       BstXI       BstYI       BstZ17I     
Bsu36I      Cac8I       CelII       CfoI        Cfr10I      
CpoI        DdeI        DraI        DraII       DraIII      
DrdI        DsaI        EaeI        EagI        Eam1105I    
EarI        EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoO109I    EcoO65I     EcoRI       EcoRII      
EcoRV       EcoT14I     EcoT22I     FbaI        FseI        
FspI        HaeII       HapII       HgaI        HhaI        
Hin1I       HinP1I      HindIII     HpaI        HpaII       
KasI        Ksp632I     KspI        MaeI        MaeIII      
MamI        MboII       MfeI        MflI        MluI        
MluNI       MroI        MscI        MslI        MspI        
MunI        MvaI        MvnI        NaeI        NarI        
NciI        NcoI        NdeI        NgoMIV      NheI        
NotI        NruI        NsiI        NspI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PleI        PmaCI       PmeI        PmlI        PpuMI       
PshAI       PshBI       Psp1406I    PstI        PvuI        
PvuII       RcaI        RsrII       SacI        SacII       
SalI        SapI        SbfI        ScaI        ScrFI       
SexAI       SfcI        SfiI        SfoI        SfuI        
SgrAI       SmaI        SmlI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        StuI        
StyI        SwaI        Tsp45I      Tsp509I     TspRI       
Tth111I     Van91I      XbaI        XcmI        XhoI        
XhoII       XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   46 enzymes) #
###################################

Acc65I        229  GGTACC               G^GTACC
AciI          246  GCGG                 CCGC(-3/-1)
AluI          254  AGCT                 AG^CT
Alw44I        128  GTGCAC               G^TGCAC
ApaLI         128  GTGCAC               G^TGCAC
Asp700I        21  GAAAAGGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I       229  GGTACC               G^GTACC
AspHI         128  GTGCAC               G[AT]GC[AT]^C
AvaI          149  CTCGGG               C^[CT]CG[GA]G
BanI          229  GGTACC               G^G[CT][GA]CC
BbvI          243  GCAGC                GCAGC(8/12)
BmyI          128  GTGCAC               G[AGT]GC[ACT]^C
BsaJI         310  CCTCGG               C^C[ACGT][ACGT]GG
BsgI          224  GTGCAG               GTGCAG(16/14)
BsiHKAI       128  GTGCAC               G[AT]GC[AT]^C
BsmAI         219  GAGAC                GTCTC(1/5)
BsmBI         219  GAGACG               CGTCTC(1/5)
BsoBI         149  CTCGGG               C^[CT]CG[GA]G
Bsp1286I      128  GTGCAC               G[AGT]GC[ACT]^C
BspDI          44  ATCGAT               AT^CGAT
BspMI         226  GCAGGT               ACCTGC(4/8)
BstF5I         15  CATCC                GGATG(2/0)
Cfr13I        314  GGCCC                G^G[ACGT]CC
ClaI           44  ATCGAT               AT^CGAT
DpnII          43  GATC                 ^GATC
FauI          246  GCGGG                CCCGC(4/6)
Fnu4HI        243  GCAGC                GC^[ACGT]GC
FokI           15  CATCC                GGATG(9/13)
HincII         51  GTTGAC               GT[CT]^[GA]AC
HindII         51  GTTGAC               GT[CT]^[GA]AC
HinfI         137  GAATC                G^A[ACGT]TC
ItaI          243  GCAGC                GC^[ACGT]GC
KpnI          229  GGTACC               GGTAC^C
MaeII         222  ACGT                 A^CGT
MboI           43  GATC                 ^GATC
MseI           35  TTAA                 T^TAA
MspA1I        244  CAGCGG               C[AC]G^C[GT]G
MwoI          246  GCGGGATAAGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NdeII          43  GATC                 ^GATC
Sau3AI         43  GATC                 ^GATC
Sau96I        314  GGCCC                G^G[ACGT]CC
SfaNI          95  GATGC                GCATC(5/9)
TfiI          137  GAATC                G^A[AT]TC
Tru9I          35  TTAA                 T^TAA
TseI          243  GCAGC                G^C[AT]GC
XmnI           21  GAAAAGGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (    8 enzymes) #
###################################

AfaI          230  GTAC                 GT^AC
              273  GTAC                
HaeIII         31  GGCC                 GG^CC
              314  GGCC                
HphI           67  GGTGA                GGTGA(8/7)
              288  TCACC               
NlaIII        100  CATG                 CATG^
              133  CATG                
NlaIV         229  GGTACC               GG[ACGT]^[ACGT]CC
              306  GGAGCC              
RsaI          230  GTAC                 GT^AC
              273  GTAC                
TaqI           45  TCGA                 T^CGA
               93  TCGA                
TthHB8I        45  TCGA                 T^CGA
               93  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (    1 enzymes) #
#########################################

MnlI