Restriction map for
pFN21AE2023
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# NO CUT ENZYMES ( 104 enzymes) #
##################################
AatII Acc65I AclI AflII AgeI
Alw44I ApaLI AscI AseI AsnI
Asp718I AspI AviII AvrII BamHI
BbeI BbrPI BciVI BclI BfrI
BglII BlnI BlpI Bpu1102I BsaAI
BsaBI BsiEI BsmI Bsp1407I BspHI
BspLU11I BspMI BsrDI BsrGI BssHII
BssSI BstBI BstEII BstPI BstXI
CelII CpoI DraI DrdI EagI
EclXI Eco52I EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HincII
HindII HindIII HpaI KasI KpnI
MamI MfeI MseI MunI NarI
NdeI NheI NotI NruI NsiI
NspI NspV PacI PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SalI
SapI SbfI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI Tru9I
Tth111I XbaI XmaCI XmaI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AccI 1668 GTATAC GT^[AC][GT]AC
AccIII 164 TCCGGA T^CCGGA
AcsI 61 GAATTT [GA]^AATT[CT]
AcyI 217 GGCGTC G[GA]^CG[CT]C
AflIII 642 ACGCGT A^C[GA][CT]GT
AhdI 407 GACCAGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 346 CCTAAGG CC^T[ACGT]AGG
ApoI 61 GAATTT [GA]^AATT[CT]
Asp700I 768 GAAGCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 407 GACCAGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbsI 460 GTCTTC GAAGAC(2/6)
BcgI 1205 GCAGGAGCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BpuAI 460 GTCTTC GAAGAC(2/6)
BsaHI 217 GGCGTC G[GA]^CG[CT]C
BsaI 405 GAGACC GGTCTC(1/5)
BseAI 164 TCCGGA T^CCGGA
BsiWI 1286 CGTACG C^GTACG
BsmBI 1290 CGTCTC CGTCTC(1/5)
BspDI 418 ATCGAT AT^CGAT
BspEI 164 TCCGGA T^CCGGA
Bst1107I 1668 GTATAC GTA^TAC
BstAPI 588 GCACATCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I 1668 GTATAC GTA^TAC
Bsu36I 346 CCTAAGG CC^T[ACGT]AGG
ClaI 418 ATCGAT AT^CGAT
DraIII 1699 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 407 GACCAGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 379 GAAGAG CTCTTC(1/4)
Eco81I 346 CCTAAGG CC^T[ACGT]AGG
Hin1I 217 GGCGTC G[GA]^CG[CT]C
Ksp632I 379 GAAGAG CTCTTC(1/4)
KspI 328 CCGCGG CCGC^GG
MaeIII 740 GTGAC ^GT[ACGT]AC
MluI 642 ACGCGT A^CGCGT
MroI 164 TCCGGA T^CCGGA
PaeR7I 1532 CTCGAG C^TCGAG
PpuMI 1162 GGGTCCC [GA]G^G[AT]CC[CT]
PvuII 1750 CAGCTG CAG^CTG
SacII 328 CCGCGG CCGC^GG
ScaI 1538 AGTACT AGT^ACT
SexAI 1700 ACCTGGT A^CC[AT]GGT
SmlI 1532 CTCGAG C^T[CT][GA]AG
StuI 125 AGGCCT AGG^CCT
Tsp45I 740 GTGAC ^GT[GC]AC
Tsp509I 62 AATT ^AATT
XcmI 233 CCAACACGGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1532 CTCGAG C^TCGAG
XmnI 768 GAAGCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
Aor51HI 770 AGCGCT AGC^GCT
989 AGCGCT
BanI 88 GGCACC G^G[CT][GA]CC
340 GGCACC
BbvCI 310 CCTCAGC CCTCAGC(-5/-2)
118 GCTGAGG
BfaI 322 CTAG C^TAG
1220 CTAG
BglI 80 GCCGTCAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1622 GCCCGTGGGGC
BsaWI 164 TCCGGA [AT]^CCGG[AT]
297 ACCGGA
BsgI 1119 GTGCAG GTGCAG(16/14)
1597 GTGCAG
BsmAI 405 GAGAC GTCTC(1/5)
1291 GTCTC
BsrFI 845 GCCGGC [GA]^CCGG[CT]
1064 GCCGGC
Cfr10I 845 GCCGGC [GA]^CCGG[CT]
1064 GCCGGC
Eco47III 770 AGCGCT AGC^GCT
989 AGCGCT
MaeI 322 CTAG C^TAG
1220 CTAG
NaeI 845 GCCGGC GCC^GGC
1064 GCCGGC
NcoI 444 CCATGG C^CATGG
1469 CCATGG
NgoMIV 845 GCCGGC G^CCGGC
1064 GCCGGC
PstI 142 CTGCAG CTGCA^G
205 CTGCAG
SacI 1006 GAGCTC GAGCT^C
1429 GAGCTC
SfaNI 476 GCATC GCATC(5/9)
1153 GCATC
TfiI 680 GATTC G^A[AT]TC
956 GATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 87 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI ApaI AspHI AvaI AvaII
BalI BanII BbvI BcnI BmyI
BpmI BsaJI BseRI BsiHKAI BsiYI
BslI BsmFI BsoBI Bsp1286I BsrBI
BsrI BssKI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII HphI
ItaI MaeII MboI MboII MflI
MluNI MnlI MscI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PflMI PleI
RsaI Sau3AI Sau96I ScrFI SfcI
StyI TaqI TseI TspRI TthHB8I
Van91I XhoII