Restriction map for
pFN21AE1247
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# NO CUT ENZYMES ( 122 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I Aor51HI ApaLI ApoI AscI
AseI AsnI Asp718I AspEI AvaI
AviII AvrII BbrPI BbvCI BcgI
BclI BfrI BglII BlnI BlpI
Bpu1102I BsaAI BsaWI BseAI BsiEI
BsiWI BsmBI BsmI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrDI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstZ17I CelII ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoRI
EcoRV EcoT14I EcoT22I FbaI FseI
FspI HgaI HincII HindII HpaI
KpnI KspI MfeI MluI MroI
MslI MunI NcoI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI ScaI SexAI
SfiI SfuI SgrAI SmaI SnaBI
SpeI SphI SspBI SspI StuI
StyI SwaI XbaI XcmI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AcyI 34 GGCGCC G[GA]^CG[CT]C
ApaI 367 GGGCCC GGGCC^C
AspI 1425 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 2 TGGCCA TGG^CCA
BamHI 97 GGATCC G^GATCC
BanI 34 GGCGCC G^G[CT][GA]CC
BbeI 34 GGCGCC GGCGC^C
BbsI 166 GAAGAC GAAGAC(2/6)
BciVI 350 GGATAC GTATCC(6/5)
BglI 37 GCCGCCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 166 GAAGAC GAAGAC(2/6)
BsaBI 877 GATGCCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 34 GGCGCC G[GA]^CG[CT]C
BsaI 1125 GAGACC GGTCTC(1/5)
BsgI 1219 GTGCAG GTGCAG(16/14)
BspMI 1411 GCAGGT ACCTGC(4/8)
BsrFI 962 GCCGGC [GA]^CCGG[CT]
BstEII 193 GGTGACC G^GT[ACGT]ACC
BstPI 193 GGTGACC G^GT[ACGT]ACC
Cfr10I 962 GCCGGC [GA]^CCGG[CT]
EaeI 2 TGGCCA [CT]^GGCC[GA]
EcoO65I 193 GGTGACC G^GT[ACGT]ACC
FauI 26 GCGGG CCCGC(4/6)
HaeII 34 GGCGCC [GA]GCGC^[CT]
Hin1I 34 GGCGCC G[GA]^CG[CT]C
HindIII 733 AAGCTT A^AGCTT
HphI 193 GGTGA GGTGA(8/7)
KasI 34 GGCGCC G^GCGCC
MamI 877 GATGCCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 2 TGGCCA TGG^CCA
MscI 2 TGGCCA TGG^CCA
NaeI 962 GCCGGC GCC^GGC
NarI 34 GGCGCC GG^CGCC
NgoMIV 962 GCCGGC G^CCGGC
NspI 285 GCATGT [GA]CATG^[CT]
PflFI 1425 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 968 GACTC GAGTC(4/5)
SacI 1342 GAGCTC GAGCT^C
SapI 1339 GAAGAGC GCTCTTC(1/4)
SbfI 621 CCTGCAGG CCTGCA^GG
SfoI 34 GGCGCC GGC^GCC
SmlI 1433 CTCAAG C^T[CT][GA]AG
Sse8387I 621 CCTGCAGG CCTGCA^GG
TaqI 164 TCGA T^CGA
TfiI 303 GAATC G^A[AT]TC
Tth111I 1425 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 164 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AccII 22 CGCG CG^CG
48 CGCG
AfaI 631 GTAC GT^AC
1227 GTAC
AlwNI 1009 CAGGACCTG CAG[ACGT][ACGT][ACGT]^CTG
1036 CAGTTCCTG
AocI 451 CCTGAGG CC^T[ACGT]AGG
702 CCTAAGG
Asp700I 166 GAAGACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
732 GAAGCTTTTC
AspHI 400 GAGCAC G[AT]GC[AT]^C
1342 GAGCTC
BsiHKAI 400 GAGCAC G[AT]GC[AT]^C
1342 GAGCTC
BsmFI 29 GGGAC GGGAC(10/14)
1069 GGGAC
BsrI 861 ACTGG ACTGG(1/-1)
978 CCAGT
BstUI 22 CGCG CG^CG
48 CGCG
BstXI 933 CCAGAGGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1373 CCAAGAGCCTGG
Bsu36I 451 CCTGAGG CC^T[ACGT]AGG
702 CCTAAGG
CfoI 21 GCGC GCG^C
35 GCGC
DsaI 335 CCACGG C^C[GA][CT]GG
371 CCGTGG
Eco81I 451 CCTGAGG CC^T[ACGT]AGG
702 CCTAAGG
HhaI 21 GCGC GCG^C
35 GCGC
HinP1I 21 GCGC G^CGC
35 GCGC
HinfI 303 GAATC G^A[ACGT]TC
968 GACTC
MaeII 404 ACGT A^CGT
797 ACGT
MseI 157 TTAA T^TAA
1270 TTAA
MvnI 22 CGCG CG^CG
48 CGCG
PflMI 549 CCAAGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1008 CCAGGACCTGG
PstI 622 CTGCAG CTGCA^G
1081 CTGCAG
RsaI 631 GTAC GT^AC
1227 GTAC
SfaNI 877 GATGC GCATC(5/9)
1393 GATGC
SfcI 622 CTGCAG C^T[GA][CT]AG
1081 CTGCAG
Tru9I 157 TTAA T^TAA
1270 TTAA
Tsp509I 73 AATT ^AATT
518 AATT
TspRI 317 CAGTG CA[GC]TG[ACGT][ACGT]^
541 CAGTG
Van91I 549 CCAAGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1008 CCAGGACCTGG
XmnI 166 GAAGACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
732 GAAGCTTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 58 enzymes) #
#########################################
AciI AluI AlwI AvaII BanII
BbvI BcnI BfaI BmyI BpmI
BsaJI BseRI BsiYI BslI BsmAI
Bsp1286I BssKI BstF5I BstNI BstYI
Cac8I Cfr13I DdeI DpnII DraII
EarI EcoNI EcoO109I EcoRII Fnu4HI
FokI HaeIII HapII HpaII ItaI
Ksp632I MaeI MaeIII MboI MboII
MflI MnlI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PpuMI PvuII Sau3AI Sau96I ScrFI
TseI Tsp45I XhoII