##################################
# NO CUT ENZYMES (  168 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcsI        AcyI        AfaI        AflII       
AflIII      AgeI        AhdI        AlwI        AlwNI       
Aor51HI     ApoI        AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AspI        AvaI        
AviII       AvrII       BamHI       BbeI        BbrPI       
BbsI        BciVI       BclI        BfaI        BfrI        
BglI        BglII       BlnI        BpuAI       BsaAI       
BsaBI       BsaHI       BsaI        BsaWI       BseAI       
BsiEI       BsiWI       BsmAI       BsmBI       BsmI        
BsoBI       Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BsrBI       BsrDI       BsrGI       BssHII      
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstPI       BstUI       BstYI       BstZ17I     CfoI        
ClaI        CpoI        DpnII       DraI        DrdI        
DsaI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      EcoO65I     EcoRI       EcoRV       EcoT22I     
FbaI        FseI        FspI        HaeII       HgaI        
HhaI        Hin1I       HinP1I      HindIII     HpaI        
HphI        KasI        KpnI        KspI        MaeI        
MaeII       MamI        MboI        MfeI        MflI        
MluI        MroI        MseI        MslI        MunI        
MvnI        NaeI        NarI        NcoI        NdeI        
NdeII       NgoMIV      NheI        NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PmaCI       PmeI        PmlI        
PshBI       Psp1406I    PvuI        RcaI        RsaI        
RsrII       SacII       SalI        SapI        Sau3AI      
SbfI        ScaI        SexAI       SfiI        SfoI        
SfuI        SgrAI       SmaI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        SwaI        
TaqI        Tru9I       Tsp509I     Tth111I     TthHB8I     
Van91I      XbaI        XcmI        XhoI        XhoII       
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   29 enzymes) #
###################################

Alw44I        149  GTGCAC               G^TGCAC
AocI          207  CCTCAGG              CC^T[ACGT]AGG
ApaI          276  GGGCCC               GGGCC^C
ApaLI         149  GTGCAC               G^TGCAC
BbvCI         328  GCTGAGG              CCTCAGC(-5/-2)
BcgI          683  CGACCAGCCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlpI          480  GCTTAGC              GC^T[ACGT]AGC
Bpu1102I      480  GCTTAGC              GC^T[ACGT]AGC
BsgI          436  GTGCAG               GTGCAG(16/14)
BsrFI         131  ACCGGC               [GA]^CCGG[CT]
BsrI          587  ACTGG                ACTGG(1/-1)
Bsu36I        207  CCTCAGG              CC^T[ACGT]AGG
CelII         480  GCTTAGC              GC^T[ACGT]AGC
Cfr10I        131  ACCGGC               [GA]^CCGG[CT]
DraIII        586  CACTGGGTG            CAC[ACGT][ACGT][ACGT]^GTG
Eco81I        207  CCTCAGG              CC^T[ACGT]AGG
FauI           52  CCCGC                CCCGC(4/6)
HincII        730  GTTGAC               GT[CT]^[GA]AC
HindII        730  GTTGAC               GT[CT]^[GA]AC
MaeIII        313  GTGAC                ^GT[ACGT]AC
NspI          755  ACATGC               [GA]CATG^[CT]
PleI          460  GAGTC                GAGTC(4/5)
PpuMI         698  GGGACCT              [GA]G^G[AT]CC[CT]
PshAI         642  GACCCGTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII         232  CAGCTG               CAG^CTG
SacI           75  GAGCTC               GAGCT^C
SfaNI         307  GATGC                GCATC(5/9)
TfiI          360  GATTC                G^A[AT]TC
Tsp45I        313  GTGAC                ^GT[GC]AC


###################################
# TWO CUT ENZYMES (   14 enzymes) #
###################################

BalI          188  TGGCCA               TGG^CCA
              396  TGGCCA              
BcnI          168  CCGGG                CC^[GC]GG
              336  CCCGG               
BseRI         652  CTCCTC               GAGGAG(10/8)
              370  GAGGAG              
BstXI         191  CCAAGACAGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              220  CCATCCTTCTGG        
EaeI          188  TGGCCA               [CT]^GGCC[GA]
              396  TGGCCA              
EcoNI         203  CCTTCCTCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
              570  CCTGCAGCAGG         
HinfI         360  GATTC                G^A[ACGT]TC
              460  GAGTC               
MluNI         188  TGGCCA               TGG^CCA
              396  TGGCCA              
MscI          188  TGGCCA               TGG^CCA
              396  TGGCCA              
NciI          168  CCGGG                CC^[GC]GG
              336  CCCGG               
NlaIII         29  CATG                 CATG^
              756  CATG                
PstI          262  CTGCAG               CTGCA^G
              571  CTGCAG              
SfcI          262  CTGCAG               C^T[GA][CT]AG
              571  CTGCAG              
StuI          173  AGGCCT               AGG^CCT
              540  AGGCCT              


#########################################
# 3 OR MORE CUT ENZYMES (   47 enzymes) #
#########################################

AciI        AluI        AspHI       AvaII       BanI        
BanII       BbvI        BmyI        BpmI        BsaJI       
BsiHKAI     BsiYI       BslI        BsmFI       Bsp1286I    
BspMI       BssKI       BstF5I      BstNI       Cac8I       
Cfr13I      DdeI        DraII       EarI        EcoO109I    
EcoRII      EcoT14I     Fnu4HI      FokI        HaeIII      
HapII       HpaII       ItaI        Ksp632I     MboII       
MnlI        MspA1I      MspI        MvaI        MwoI        
NlaIV       Sau96I      ScrFI       SmlI        StyI        
TseI        TspRI