Restriction map for
pFN21AE1743
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# NO CUT ENZYMES ( 149 enzymes) #
##################################
AatII Acc65I AccI AccII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I AlwI AocI Aor51HI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AviII AvrII
BamHI BbeI BbrPI BbvCI BciVI
BclI BfaI BfrI BglI BglII
BlnI BlpI BpmI Bpu1102I BsaAI
BsaBI BsaHI BsiWI BsmI Bsp1407I
BspDI BspHI BspLU11I BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstUI BstYI
BstZ17I Bsu36I CelII CfoI Cfr10I
ClaI CpoI DpnII DraI DraIII
DrdI Eam1105I Eco47III Eco81I EcoRI
EcoRV EcoT22I FauI FbaI FseI
FspI HaeII HhaI Hin1I HinP1I
HincII HindII HindIII HpaI KasI
KpnI KspI MaeI MamI MboI
MfeI MflI MluI MslI MunI
MvnI NaeI NarI NcoI NdeI
NdeII NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflMI PinAI PleI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
Sau3AI SbfI ScaI SfiI SfoI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI Van91I
XbaI XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AccIII 176 TCCGGA T^CCGGA
AluI 623 AGCT AG^CT
AlwNI 490 CAGGTGCTG CAG[ACGT][ACGT][ACGT]^CTG
BalI 38 TGGCCA TGG^CCA
BanI 301 GGCACC G^G[CT][GA]CC
BcgI 448 CGATTCGTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaI 203 GAGACC GGTCTC(1/5)
BsaWI 176 TCCGGA [AT]^CCGG[AT]
BseAI 176 TCCGGA T^CCGGA
BsgI 456 CTGCAC GTGCAG(16/14)
BsiEI 444 CGGCCG CG[GA][CT]^CG
BsmBI 525 CGTCTC CGTCTC(1/5)
BspEI 176 TCCGGA T^CCGGA
BsrI 501 CCAGT ACTGG(1/-1)
BstXI 41 CCATATCGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DdeI 689 CTGAG C^T[ACGT]AG
DsaI 279 CCGTGG C^C[GA][CT]GG
EagI 444 CGGCCG C^GGCCG
EarI 589 CTCTTC CTCTTC(1/4)
EclXI 444 CGGCCG C^GGCCG
Eco52I 444 CGGCCG C^GGCCG
EcoNI 530 CCTCCTCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HinfI 449 GATTC G^A[ACGT]TC
Ksp632I 589 CTCTTC CTCTTC(1/4)
MaeII 386 ACGT A^CGT
MluNI 38 TGGCCA TGG^CCA
MroI 176 TCCGGA T^CCGGA
MscI 38 TGGCCA TGG^CCA
NlaIII 612 CATG CATG^
PpuMI 6 GGGTCCC [GA]G^G[AT]CC[CT]
PstI 625 CTGCAG CTGCA^G
PvuII 622 CAGCTG CAG^CTG
SapI 588 GCTCTTC GCTCTTC(1/4)
SexAI 638 ACCAGGT A^CC[AT]GGT
SfcI 625 CTGCAG C^T[GA][CT]AG
TaqI 398 TCGA T^CGA
TfiI 449 GATTC G^A[AT]TC
Tsp509I 294 AATT ^AATT
TthHB8I 398 TCGA T^CGA
XcmI 207 CCAGGGGGAACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AfaI 429 GTAC GT^AC
463 GTAC
AspHI 25 GTGCTC G[AT]GC[AT]^C
80 GAGCAC
AvaI 11 CCCGGG C^[CT]CG[GA]G
288 CCCGGG
BbsI 110 GAAGAC GAAGAC(2/6)
703 GTCTTC
BpuAI 110 GAAGAC GAAGAC(2/6)
703 GTCTTC
BseRI 528 CTCCTC GAGGAG(10/8)
531 CTCCTC
BsiHKAI 25 GTGCTC G[AT]GC[AT]^C
80 GAGCAC
BsmAI 203 GAGAC GTCTC(1/5)
526 GTCTC
BsmFI 8 GTCCC GGGAC(10/14)
498 GTCCC
BsoBI 11 CCCGGG C^[CT]CG[GA]G
288 CCCGGG
BstEII 60 GGTGACC G^GT[ACGT]ACC
634 GGTGACC
BstPI 60 GGTGACC G^GT[ACGT]ACC
634 GGTGACC
EcoO65I 60 GGTGACC G^GT[ACGT]ACC
634 GGTGACC
EcoT14I 189 CCAAGG C^C[AT][AT]GG
305 CCAAGG
HgaI 551 GACGC GACGC(5/10)
701 GCGTC
MseI 197 TTAA T^TAA
296 TTAA
RsaI 429 GTAC GT^AC
463 GTAC
SmaI 11 CCCGGG CCC^GGG
288 CCCGGG
SmlI 139 CTCAAG C^T[CT][GA]AG
534 CTCAAG
StuI 135 AGGCCT AGG^CCT
192 AGGCCT
StyI 189 CCAAGG C^C[AT][AT]GG
305 CCAAGG
Tru9I 197 TTAA T^TAA
296 TTAA
TspRI 459 CACTG CA[GC]TG[ACGT][ACGT]^
502 CAGTG
XmaCI 11 CCCGGG C^CCGGG
288 CCCGGG
XmaI 11 CCCGGG C^CCGGG
288 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 44 enzymes) #
#########################################
AciI ApaI AspI AvaII BanII
BbvI BcnI BmyI BsaJI BsiYI
BslI Bsp1286I BssKI BstF5I BstNI
Cac8I Cfr13I DraII EaeI EcoO109I
EcoRII Fnu4HI FokI HaeIII HapII
HpaII HphI ItaI MaeIII MboII
MnlI MspA1I MspI MvaI MwoI
NciI NlaIV PflFI Sau96I ScrFI
SfaNI TseI Tsp45I Tth111I