Restriction map for
pFN21AB7796
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# NO CUT ENZYMES ( 116 enzymes) #
##################################
AatII AccI AclI AcsI AflII
AflIII AgeI AhdI Aor51HI ApaI
ApoI AscI AseI AsnI Asp700I
AspEI AvaI AviII AvrII BamHI
BbrPI BciVI BclI BfaI BfrI
BglII BlnI BlpI Bpu1102I BsaAI
BsiWI BsmI BsoBI Bsp1407I BspDI
BspLU11I BsrBI BsrDI BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstZ17I CelII ClaI CpoI DraI
DraIII DrdI Eam1105I EarI Eco47III
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HincII HindII HindIII
HpaI Ksp632I KspI MaeI MaeII
MfeI MluI MseI MunI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfuI
SgrAI SmaI SmlI SnaBI SpeI
Sse8387I SspBI SspI SwaI Tru9I
Van91I XbaI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
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Acc65I 21 GGTACC G^GTACC
AccIII 333 TCCGGA T^CCGGA
Alw44I 961 GTGCAC G^TGCAC
AlwI 396 GATCC GGATC(4/5)
AocI 1144 CCTCAGG CC^T[ACGT]AGG
ApaLI 961 GTGCAC G^TGCAC
Asp718I 21 GGTACC G^GTACC
BalI 99 TGGCCA TGG^CCA
BbeI 1101 GGCGCC GGCGC^C
BbsI 130 GTCTTC GAAGAC(2/6)
BbvCI 970 CCTCAGC CCTCAGC(-5/-2)
BcgI 26 CGAGAATGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 1382 GCCAGTACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 130 GTCTTC GAAGAC(2/6)
BsaBI 1152 GATGAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 274 GAGACC GGTCTC(1/5)
BsaWI 333 TCCGGA [AT]^CCGG[AT]
BseAI 333 TCCGGA T^CCGGA
BsgI 775 GTGCAG GTGCAG(16/14)
BsmBI 246 CGTCTC CGTCTC(1/5)
BspEI 333 TCCGGA T^CCGGA
BspHI 1088 TCATGA T^CATGA
BspMI 777 GCAGGT ACCTGC(4/8)
BstAPI 1015 GCAGCGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 395 AGATCC [GA]^GATC[CT]
Bsu36I 1144 CCTCAGG CC^T[ACGT]AGG
DpnII 396 GATC ^GATC
Eco81I 1144 CCTCAGG CC^T[ACGT]AGG
EcoNI 698 CCTTTGAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HaeII 1101 GGCGCC [GA]GCGC^[CT]
KasI 1101 GGCGCC G^GCGCC
KpnI 21 GGTACC GGTAC^C
MamI 1152 GATGAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboI 396 GATC ^GATC
MflI 395 AGATCC [GA]^GATC[CT]
MluNI 99 TGGCCA TGG^CCA
MroI 333 TCCGGA T^CCGGA
MscI 99 TGGCCA TGG^CCA
MslI 876 CACCGGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 1101 GGCGCC GG^CGCC
NcoI 192 CCATGG C^CATGG
NdeII 396 GATC ^GATC
PpuMI 706 AGGACCC [GA]G^G[AT]CC[CT]
RcaI 1088 TCATGA T^CATGA
Sau3AI 396 GATC ^GATC
SfiI 1381 GGCCAGTACGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 1101 GGCGCC GGC^GCC
SphI 881 GCATGC GCATG^C
Tsp45I 428 GTGAC ^GT[GC]AC
Tsp509I 1523 AATT ^AATT
XcmI 1505 CCATTTCACAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 395 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AcyI 1101 GGCGCC G[GA]^CG[CT]C
1416 GGCGTC
AlwNI 973 CAGCCCCTG CAG[ACGT][ACGT][ACGT]^CTG
1016 CAGCGGCTG
AspHI 469 GTGCTC G[AT]GC[AT]^C
961 GTGCAC
AspI 276 GACCGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
647 GACGCAGTC
BanII 597 GGGCTC G[GA]GC[CT]^C
1052 GAGCCC
BsaHI 1101 GGCGCC G[GA]^CG[CT]C
1416 GGCGTC
BsiEI 37 CGGCCG CG[GA][CT]^CG
668 CGGCCG
BsiHKAI 469 GTGCTC G[AT]GC[AT]^C
961 GTGCAC
BsrI 1172 CCAGT ACTGG(1/-1)
1383 CCAGT
DraII 706 AGGACCC [GA]G^G[ACGT]CC[CT]
1450 AGGGCCT
EagI 37 CGGCCG C^GGCCG
668 CGGCCG
EclXI 37 CGGCCG C^GGCCG
668 CGGCCG
Eco52I 37 CGGCCG C^GGCCG
668 CGGCCG
EcoO109I 706 AGGACCC [GA]G^G[ACGT]CC[CT]
1450 AGGGCCT
EcoT14I 102 CCAAGG C^C[AT][AT]GG
192 CCATGG
Hin1I 1101 GGCGCC G[GA]^CG[CT]C
1416 GGCGTC
HphI 1149 GGTGA GGTGA(8/7)
1550 TCACC
MaeIII 428 GTGAC ^GT[ACGT]AC
791 GTAAC
MboII 489 GAAGA GAAGA(8/7)
131 TCTTC
NaeI 39 GCCGGC GCC^GGC
1366 GCCGGC
NgoMIV 39 GCCGGC G^CCGGC
1366 GCCGGC
NspI 380 GCATGT [GA]CATG^[CT]
881 GCATGC
PflFI 276 GACCGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
647 GACGCAGTC
StuI 695 AGGCCT AGG^CCT
1313 AGGCCT
StyI 102 CCAAGG C^C[AT][AT]GG
192 CCATGG
TaqI 457 TCGA T^CGA
557 TCGA
Tth111I 276 GACCGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
647 GACGCAGTC
TthHB8I 457 TCGA T^CGA
557 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 62 enzymes) #
#########################################
AccII AciI AfaI AluI AvaII
BanI BbvI BcnI BmyI BpmI
BsaJI BseRI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrFI BssKI BstF5I
BstNI BstUI BstXI Cac8I CfoI
Cfr10I Cfr13I DdeI DsaI EaeI
EcoRII FauI Fnu4HI FokI HaeIII
HapII HgaI HhaI HinP1I HinfI
HpaII ItaI MnlI MspA1I MspI
MvaI MvnI MwoI NciI NlaIII
NlaIV PleI PstI PvuII RsaI
Sau96I ScrFI SfaNI SfcI TfiI
TseI TspRI