Restriction map for
pFN21AE5336
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# NO CUT ENZYMES ( 146 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcsI AflII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvrII BalI BanII
BbeI BbvCI BcgI BciVI BclI
BfrI BglI BlnI BlpI Bpu1102I
BsaBI BsaI BseAI BsgI BsiEI
BsiWI BsmAI BsmBI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstUI BstZ17I Bsu36I
CelII ClaI CpoI DraI DraIII
DrdI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
HaeII HincII HindII HpaI KasI
KpnI Ksp632I KspI MamI MfeI
MluI MluNI MroI MscI MseI
MunI MvnI NaeI NarI NdeI
NgoMIV NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmeI PpuMI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI TfiI Tru9I Tsp509I
Tth111I Van91I XbaI XcmI XhoI
XmnI
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# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AccI 751 GTCTAC GT^[AC][GT]AC
AcyI 619 GGCGTC G[GA]^CG[CT]C
AfaI 144 GTAC GT^AC
AlwNI 523 CAGGGCCTG CAG[ACGT][ACGT][ACGT]^CTG
AspHI 555 GTGCTC G[AT]GC[AT]^C
AviII 353 TGCGCA TGC^GCA
BamHI 292 GGATCC G^GATCC
BanI 54 GGTGCC G^G[CT][GA]CC
BbsI 280 GAAGAC GAAGAC(2/6)
BfaI 194 CTAG C^TAG
BglII 131 AGATCT A^GATCT
BpuAI 280 GAAGAC GAAGAC(2/6)
BsaHI 619 GGCGTC G[GA]^CG[CT]C
BsaWI 646 TCCGGT [AT]^CCGG[AT]
BseRI 95 GAGGAG GAGGAG(10/8)
BsiHKAI 555 GTGCTC G[AT]GC[AT]^C
BsmI 339 GAATGC GAATGC(1/-1)
BstAPI 522 GCAGGGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 407 CCATCCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraII 524 AGGGCCT [GA]G^G[ACGT]CC[CT]
EaeI 251 TGGCCG [CT]^GGCC[GA]
EcoO109I 524 AGGGCCT [GA]G^G[ACGT]CC[CT]
FspI 353 TGCGCA TGC^GCA
HgaI 620 GCGTC GACGC(5/10)
Hin1I 619 GGCGTC G[GA]^CG[CT]C
HindIII 376 AAGCTT A^AGCTT
MaeI 194 CTAG C^TAG
NcoI 596 CCATGG C^CATGG
NheI 193 GCTAGC G^CTAGC
NspI 387 GCATGT [GA]CATG^[CT]
RsaI 144 GTAC GT^AC
SmaI 758 CCCGGG CCC^GGG
SmlI 148 CTCAAG C^T[CT][GA]AG
TaqI 776 TCGA T^CGA
TthHB8I 776 TCGA T^CGA
XmaCI 758 CCCGGG C^CCGGG
XmaI 758 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AflIII 158 ACACGT A^C[GA][CT]GT
172 ACGTGT
AvaI 83 CCCGAG C^[CT]CG[GA]G
758 CCCGGG
AvaII 239 GGACC G^G[AT]CC
491 GGACC
BbrPI 159 CACGTG CAC^GTG
552 CACGTG
BmyI 55 GTGCCC G[AGT]GC[ACT]^C
555 GTGCTC
BsmFI 181 GGGAC GGGAC(10/14)
641 GGGAC
BsoBI 83 CCCGAG C^[CT]CG[GA]G
758 CCCGGG
Bsp1286I 55 GTGCCC G[AGT]GC[ACT]^C
555 GTGCTC
BsrFI 472 ACCGGC [GA]^CCGG[CT]
744 GCCGGT
BstYI 131 AGATCT [GA]^GATC[CT]
292 GGATCC
Cfr10I 472 ACCGGC [GA]^CCGG[CT]
744 GCCGGT
DdeI 397 CTGAG C^T[ACGT]AG
627 CTCAG
EcoT14I 449 CCAAGG C^C[AT][AT]GG
596 CCATGG
HinfI 436 GACTC G^A[ACGT]TC
643 GACTC
HphI 585 GGTGA GGTGA(8/7)
470 TCACC
MaeIII 401 GTGAC ^GT[ACGT]AC
469 GTCAC
MflI 131 AGATCT [GA]^GATC[CT]
292 GGATCC
NlaIII 388 CATG CATG^
597 CATG
PleI 436 GACTC GAGTC(4/5)
643 GACTC
PmaCI 159 CACGTG CAC^GTG
552 CACGTG
PmlI 159 CACGTG CAC^GTG
552 CACGTG
PstI 517 CTGCAG CTGCA^G
529 CTGCAG
SfcI 517 CTGCAG C^T[GA][CT]AG
529 CTGCAG
StyI 449 CCAAGG C^C[AT][AT]GG
596 CCATGG
Tsp45I 401 GTGAC ^GT[GC]AC
469 GTCAC
XhoII 131 AGATCT [GA]^GATC[CT]
292 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 49 enzymes) #
#########################################
AciI AluI AlwI BbvI BcnI
BpmI BsaAI BsaJI BsiYI BslI
BsrDI BsrI BssKI BstF5I BstNI
Cac8I CfoI Cfr13I DpnII DsaI
EcoRII FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HpaII ItaI
MaeII MboI MboII MnlI MslI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIV PvuII Sau3AI Sau96I
ScrFI SfaNI TseI TspRI