Restriction map for
pFN21AE1924
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# NO CUT ENZYMES ( 118 enzymes) #
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AatII Acc65I AccIII AclI AcsI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvrII BamHI BbrPI BbvCI
BcgI BciVI BfaI BfrI BlnI
BsaAI BsaBI BsaWI BseAI BsiEI
BsiWI Bsp1407I BspDI BspEI BspLU11I
BsrBI BsrDI BsrGI BssSI Bst1107I
BstBI BstEII BstPI BstZ17I Bsu36I
ClaI CpoI DraI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FseI
HincII HindII HindIII HpaI KpnI
KspI MaeI MamI MfeI MluI
MroI MseI MslI MunI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshBI Psp1406I
PvuI RsrII SacII SalI ScaI
SfuI SgrAI SnaBI SpeI SphI
SspBI SspI StuI SwaI TaqI
Tru9I Tsp509I Tth111I TthHB8I Van91I
XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 31 enzymes) #
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AccI 815 GTCTAC GT^[AC][GT]AC
AlwI 668 GATCC GGATC(4/5)
AviII 419 TGCGCA TGC^GCA
BbsI 193 GTCTTC GAAGAC(2/6)
BclI 959 TGATCA T^GATCA
BglII 548 AGATCT A^GATCT
BlpI 381 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 381 GCTGAGC GC^T[ACGT]AGC
BpuAI 193 GTCTTC GAAGAC(2/6)
BsaI 1312 GAGACC GGTCTC(1/5)
BseRI 1191 CTCCTC GAGGAG(10/8)
BsmBI 1303 GAGACG CGTCTC(1/5)
BsmI 576 GAATGC GAATGC(1/-1)
BspHI 848 TCATGA T^CATGA
BstXI 1004 CCAGTGCGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 548 AGATCT [GA]^GATC[CT]
CelII 381 GCTGAGC GC^T[ACGT]AGC
DraIII 216 CACCGTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1252 GACGAGCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FbaI 959 TGATCA T^GATCA
FspI 419 TGCGCA TGC^GCA
HgaI 1305 GACGC GACGC(5/10)
MflI 548 AGATCT [GA]^GATC[CT]
NcoI 1122 CCATGG C^CATGG
PshAI 1128 GACCCAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 848 TCATGA T^CATGA
SapI 775 GCTCTTC GCTCTTC(1/4)
SbfI 356 CCTGCAGG CCTGCA^GG
SexAI 308 ACCAGGT A^CC[AT]GGT
Sse8387I 356 CCTGCAGG CCTGCA^GG
XhoII 548 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 23 enzymes) #
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AlwNI 455 CAGGAACTG CAG[ACGT][ACGT][ACGT]^CTG
949 CAGCACCTG
ApaI 735 GGGCCC GGGCC^C
936 GGGCCC
AvaII 1127 GGACC G^G[AT]CC
1260 GGTCC
BbeI 3 GGCGCC GGCGC^C
1023 GGCGCC
BsrFI 119 GCCGGC [GA]^CCGG[CT]
401 GCCGGC
BssHII 377 GCGCGC G^CGCGC
410 GCGCGC
Cfr10I 119 GCCGGC [GA]^CCGG[CT]
401 GCCGGC
DsaI 524 CCACGG C^C[GA][CT]GG
1122 CCATGG
EarI 776 CTCTTC CTCTTC(1/4)
1333 GAAGAG
FauI 70 CCCGC CCCGC(4/6)
293 CCCGC
KasI 3 GGCGCC G^GCGCC
1023 GGCGCC
Ksp632I 776 CTCTTC CTCTTC(1/4)
1333 GAAGAG
NaeI 119 GCCGGC GCC^GGC
401 GCCGGC
NarI 3 GGCGCC GG^CGCC
1023 GGCGCC
NgoMIV 119 GCCGGC G^CCGGC
401 GCCGGC
NspI 115 ACATGC [GA]CATG^[CT]
183 ACATGC
PleI 226 GACTC GAGTC(4/5)
1240 GAGTC
PpuMI 1126 GGGACCC [GA]G^G[AT]CC[CT]
1259 AGGTCCC
PvuII 749 CAGCTG CAG^CTG
1297 CAGCTG
SfoI 3 GGCGCC GGC^GCC
1023 GGCGCC
SmlI 279 CTTGAG C^T[CT][GA]AG
320 CTCAAG
TfiI 1105 GATTC G^A[AT]TC
1225 GATTC
XcmI 614 CCACCCGGAGTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1202 CCAGCTCCACAATGG
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# 3 OR MORE CUT ENZYMES ( 86 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AspHI AvaI BalI BanI BanII
BbvI BcnI BglI BmyI BpmI
BsaHI BsaJI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrI BssKI BstAPI BstF5I
BstNI BstUI Cac8I CfoI Cfr13I
DdeI DpnII DraII EaeI EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeII
HaeIII HapII HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI MaeII
MaeIII MboI MboII MluNI MnlI
MscI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
PstI RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SfiI SmaI
StyI TseI Tsp45I TspRI XmaCI
XmaI