Restriction map for
pFN21AE6167
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# NO CUT ENZYMES ( 196 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI AluI Alw44I Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AspI AvaI
AviII BalI BamHI BanI BanII
BbeI BbrPI BbsI BcgI BclI
BcnI BfrI BglI BglII BmyI
BpmI BpuAI BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BseRI BsgI
BsiEI BsiHKAI BsiWI BsiYI BslI
BsmBI BsmI BsoBI Bsp1286I Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrDI BsrFI BsrGI BsrI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstYI
BstZ17I CfoI Cfr10I ClaI CpoI
DraII DraIII DrdI DsaI EaeI
EagI Eam1105I EarI EclXI Eco47III
Eco52I EcoNI EcoO109I EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HaeII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HindIII HpaI
HpaII KasI KpnI Ksp632I KspI
MaeII MaeIII MamI MfeI MflI
MluI MluNI MroI MscI MslI
MspI MunI MvnI MwoI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PleI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
PvuII RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfaNI SfcI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
Tsp45I TspRI Tth111I Van91I XbaI
XcmI XhoI XhoII XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AfaI 20 GTAC GT^AC
AlwI 329 GGATC GGATC(4/5)
AlwNI 312 CAGAGGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 171 CCTAAGG CC^T[ACGT]AGG
AvrII 69 CCTAGG C^CTAGG
BbvCI 12 CCTCAGC CCTCAGC(-5/-2)
BciVI 287 GTATCC GTATCC(6/5)
BfaI 70 CTAG C^TAG
BlnI 69 CCTAGG C^CTAGG
BlpI 33 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 33 GCTCAGC GC^T[ACGT]AGC
BsaJI 69 CCTAGG C^C[ACGT][ACGT]GG
BsmAI 338 GAGAC GTCTC(1/5)
BsmFI 266 GGGAC GGGAC(10/14)
BstF5I 153 CATCC GGATG(2/0)
Bsu36I 171 CCTAAGG CC^T[ACGT]AGG
CelII 33 GCTCAGC GC^T[ACGT]AGC
DpnII 330 GATC ^GATC
DraI 193 TTTAAA TTT^AAA
Eco81I 171 CCTAAGG CC^T[ACGT]AGG
EcoT14I 69 CCTAGG C^C[AT][AT]GG
FauI 4 CCCGC CCCGC(4/6)
FokI 153 CATCC GGATG(9/13)
HinfI 142 GATTC G^A[ACGT]TC
HphI 281 TCACC GGTGA(8/7)
MaeI 70 CTAG C^TAG
MboI 330 GATC ^GATC
MseI 194 TTAA T^TAA
MspA1I 15 CAGCGG C[AC]G^C[GT]G
NdeII 330 GATC ^GATC
NlaIII 60 CATG CATG^
NspI 59 ACATGC [GA]CATG^[CT]
RsaI 20 GTAC GT^AC
Sau3AI 330 GATC ^GATC
StuI 228 AGGCCT AGG^CCT
StyI 69 CCTAGG C^C[AT][AT]GG
TfiI 142 GATTC G^A[AT]TC
Tru9I 194 TTAA T^TAA
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# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AciI 5 CCGC CCGC(-3/-1)
17 GCGG
AcsI 190 AAATTT [GA]^AATT[CT]
208 GAATTT
ApoI 190 AAATTT [GA]^AATT[CT]
208 GAATTT
AvaII 26 GGACC G^G[AT]CC
320 GGACC
BbvI 38 GCAGC GCAGC(8/12)
167 GCAGC
BssKI 231 CCTGG ^CC[ACGT]GG
351 CCTGG
BstNI 231 CCTGG CC^[AT]GG
351 CCTGG
Cac8I 3 GCCCGC GC[ACGT]^[ACGT]GC
63 GCTGGC
EcoRII 231 CCTGG ^CC[AT]GG
351 CCTGG
Fnu4HI 38 GCAGC GC^[ACGT]GC
167 GCAGC
HaeIII 66 GGCC GG^CC
229 GGCC
ItaI 38 GCAGC GC^[ACGT]GC
167 GCAGC
MvaI 231 CCTGG CC^[AT]GG
351 CCTGG
NlaIV 26 GGACCC GG[ACGT]^[ACGT]CC
320 GGACCC
ScrFI 231 CCTGG CC^[ACGT]GG
351 CCTGG
TaqI 50 TCGA T^CGA
332 TCGA
TseI 38 GCAGC G^C[AT]GC
167 GCAGC
Tsp509I 191 AATT ^AATT
209 AATT
TthHB8I 50 TCGA T^CGA
332 TCGA
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# 3 OR MORE CUT ENZYMES ( 5 enzymes) #
#########################################
Cfr13I DdeI MboII MnlI Sau96I