Restriction map for
pFN21AE1579
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# NO CUT ENZYMES ( 174 enzymes) #
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AatII Acc65I AccI AclI AcsI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvrII
BalI BamHI BanI BbeI BbrPI
BbvCI BciVI BclI BfaI BfrI
BglI BglII BlnI BpmI BsaAI
BsaBI BsaHI BsaI BsiEI BsiWI
BsmFI BsmI BspDI BspHI BspLU11I
BspMI BsrBI BsrDI BsrFI BsrI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstF5I BstPI BstXI BstYI
BstZ17I Bsu36I Cfr10I ClaI CpoI
DraI DraII DraIII DrdI DsaI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRI EcoRV EcoT14I EcoT22I FauI
FbaI FokI FseI HaeII HaeIII
HgaI Hin1I HindIII HpaI KasI
KpnI KspI MaeI MaeIII MamI
MfeI MflI MluI MluNI MscI
MseI MslI MunI NaeI NarI
NcoI NdeI NgoMIV NheI NlaIV
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI RcaI
RsrII SacII SalI SapI SbfI
ScaI SexAI SfaNI SfcI SfiI
SfoI SfuI SgrAI SmlI SnaBI
SpeI SphI Sse8387I SspI StuI
StyI SwaI TfiI Tru9I Tsp45I
Tsp509I TspRI Tth111I Van91I XbaI
XcmI XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 61 enzymes) #
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AccII 7 CGCG CG^CG
AccIII 30 TCCGGA T^CCGGA
AfaI 42 GTAC GT^AC
AlwI 88 GGATC GGATC(4/5)
AspHI 14 GAGCTC G[AT]GC[AT]^C
AvaI 19 CCCGGG C^[CT]CG[GA]G
AvaII 57 GGTCC G^G[AT]CC
AviII 122 TGCGCA TGC^GCA
BanII 14 GAGCTC G[GA]GC[CT]^C
BbsI 229 GAAGAC GAAGAC(2/6)
BcgI 180 CGACCAGGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlpI 153 GCTCAGC GC^T[ACGT]AGC
BmyI 14 GAGCTC G[AGT]GC[ACT]^C
Bpu1102I 153 GCTCAGC GC^T[ACGT]AGC
BpuAI 229 GAAGAC GAAGAC(2/6)
BseAI 30 TCCGGA T^CCGGA
BsgI 188 GTGCAG GTGCAG(16/14)
BsiHKAI 14 GAGCTC G[AT]GC[AT]^C
BsiYI 128 CCTATGCAGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 128 CCTATGCAGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 223 GAGAC GTCTC(1/5)
BsmBI 223 GAGACG CGTCTC(1/5)
BsoBI 19 CCCGGG C^[CT]CG[GA]G
Bsp1286I 14 GAGCTC G[AGT]GC[ACT]^C
Bsp1407I 41 TGTACA T^GTACA
BspEI 30 TCCGGA T^CCGGA
BsrGI 41 TGTACA T^GTACA
BstUI 7 CGCG CG^CG
CelII 153 GCTCAGC GC^T[ACGT]AGC
CfoI 123 GCGC GCG^C
Cfr13I 57 GGTCC G^G[ACGT]CC
DdeI 154 CTCAG C^T[ACGT]AG
DpnII 89 GATC ^GATC
EarI 24 GAAGAG CTCTTC(1/4)
FspI 122 TGCGCA TGC^GCA
HhaI 123 GCGC GCG^C
HinP1I 123 GCGC G^CGC
HincII 142 GTCAAC GT[CT]^[GA]AC
HindII 142 GTCAAC GT[CT]^[GA]AC
HinfI 27 GAGTC G^A[ACGT]TC
HphI 201 GGTGA GGTGA(8/7)
Ksp632I 24 GAAGAG CTCTTC(1/4)
MaeII 197 ACGT A^CGT
MboI 89 GATC ^GATC
MroI 30 TCCGGA T^CCGGA
MspA1I 160 CAGCTG C[AC]G^C[GT]G
MvnI 7 CGCG CG^CG
NdeII 89 GATC ^GATC
NlaIII 72 CATG CATG^
PleI 27 GAGTC GAGTC(4/5)
PvuII 160 CAGCTG CAG^CTG
RsaI 42 GTAC GT^AC
SacI 14 GAGCTC GAGCT^C
Sau3AI 89 GATC ^GATC
Sau96I 57 GGTCC G^G[ACGT]CC
SmaI 19 CCCGGG CCC^GGG
SspBI 41 TGTACA T^GTACA
TaqI 179 TCGA T^CGA
TthHB8I 179 TCGA T^CGA
XmaCI 19 CCCGGG C^CCGGG
XmaI 19 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 7 enzymes) #
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BcnI 19 CCCGG CC^[GC]GG
20 CCGGG
BsaJI 19 CCCGGG C^C[ACGT][ACGT]GG
183 CCAGGG
BsaWI 30 TCCGGA [AT]^CCGG[AT]
233 ACCGGA
BstNI 183 CCAGG CC^[AT]GG
215 CCAGG
EcoRII 183 CCAGG ^CC[AT]GG
215 CCAGG
MvaI 183 CCAGG CC^[AT]GG
215 CCAGG
NciI 19 CCCGG CC^[GC]GG
20 CCGGG
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# 3 OR MORE CUT ENZYMES ( 16 enzymes) #
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AciI AluI BbvI BseRI BssKI
Cac8I Fnu4HI HapII HpaII ItaI
MboII MnlI MspI MwoI ScrFI
TseI