Restriction map for
pFN21AE3976
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# NO CUT ENZYMES ( 147 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcsI AflII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AviII AvrII BalI
BamHI BanI BbeI BbsI BbvCI
BcgI BclI BfaI BfrI BglII
BlnI BlpI Bpu1102I BpuAI BsaBI
BsaI BseAI BsiWI BsmBI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrDI BsrGI BssHII BstAPI BstBI
BstEII BstPI BstUI Bsu36I CelII
ClaI CpoI DraI DrdI EaeI
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoO65I EcoRI EcoRV
EcoT14I EcoT22I FbaI FseI FspI
HincII HindII HindIII HpaI KasI
KpnI Ksp632I KspI MaeI MamI
MboII MfeI MluI MluNI MroI
MscI MseI MunI MvnI NarI
NcoI NdeI NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmeI PshAI PshBI Psp1406I
PstI RcaI RsrII SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StyI SwaI TfiI Tru9I Tsp509I
Tth111I XbaI XcmI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AccI 276 GTATAC GT^[AC][GT]AC
AcyI 361 GACGCC G[GA]^CG[CT]C
AflIII 293 ACGTGT A^C[GA][CT]GT
AvaI 694 CCCGAG C^[CT]CG[GA]G
AvaII 45 GGACC G^G[AT]CC
BanII 73 GAGCTC G[GA]GC[CT]^C
BciVI 627 GTATCC GTATCC(6/5)
BcnI 104 CCCGG CC^[GC]GG
BglI 99 GCCCACCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaHI 361 GACGCC G[GA]^CG[CT]C
BsaWI 234 TCCGGT [AT]^CCGG[AT]
BseRI 808 CTCCTC GAGGAG(10/8)
BsoBI 694 CCCGAG C^[CT]CG[GA]G
BsrBI 706 CCGCTC CCGCTC(-3/-3)
Bst1107I 276 GTATAC GTA^TAC
BstXI 101 CCACCCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 276 GTATAC GTA^TAC
DdeI 562 CTGAG C^T[ACGT]AG
DraIII 334 CACGCCGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoNI 63 CCTGAAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FauI 324 GCGGG CCCGC(4/6)
HaeII 755 AGCGCC [GA]GCGC^[CT]
Hin1I 361 GACGCC G[GA]^CG[CT]C
HphI 225 TCACC GGTGA(8/7)
MaeIII 13 GTCAC ^GT[ACGT]AC
MslI 588 CATGGGAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 786 GCCGGC GCC^GGC
NciI 104 CCCGG CC^[GC]GG
NgoMIV 786 GCCGGC G^CCGGC
NspI 125 ACATGC [GA]CATG^[CT]
PflMI 78 CCAGGGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 44 GGGACCC [GA]G^G[AT]CC[CT]
PvuI 181 CGATCG CGAT^CG
PvuII 151 CAGCTG CAG^CTG
SacI 73 GAGCTC GAGCT^C
SfaNI 231 GCATC GCATC(5/9)
SfcI 632 CTGTAG C^T[GA][CT]AG
StuI 800 AGGCCT AGG^CCT
TaqI 689 TCGA T^CGA
Tsp45I 13 GTCAC ^GT[GC]AC
TthHB8I 689 TCGA T^CGA
Van91I 78 CCAGGGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AlwNI 79 CAGGGTCTG CAG[ACGT][ACGT][ACGT]^CTG
151 CAGCTGCTG
AspHI 73 GAGCTC G[AT]GC[AT]^C
246 GAGCAC
BbrPI 292 CACGTG CAC^GTG
306 CACGTG
BpmI 76 CTCCAG CTGGAG(16/14)
17 CTGGAG
BsgI 289 CTGCAC GTGCAG(16/14)
370 CTGCAC
BsiEI 177 CGACCG CG[GA][CT]^CG
181 CGATCG
BsiHKAI 73 GAGCTC G[AT]GC[AT]^C
246 GAGCAC
BsmAI 318 GAGAC GTCTC(1/5)
505 GAGAC
BsmI 668 GAATGC GAATGC(1/-1)
159 GCATTC
BsrI 16 ACTGG ACTGG(1/-1)
120 ACTGG
BssSI 409 CACGAG CACGAG(-5/-1)
394 CTCGTG
BstF5I 232 CATCC GGATG(2/0)
576 GGATG
Cac8I 95 GCAAGC GC[ACGT]^[ACGT]GC
786 GCCGGC
DraII 44 GGGACCC [GA]G^G[ACGT]CC[CT]
58 AGGCCCC
DsaI 282 CCGTGG C^C[GA][CT]GG
338 CCGTGG
EcoO109I 44 GGGACCC [GA]G^G[ACGT]CC[CT]
58 AGGCCCC
FokI 232 CATCC GGATG(9/13)
576 GGATG
HgaI 253 GACGC GACGC(5/10)
361 GACGC
PmaCI 292 CACGTG CAC^GTG
306 CACGTG
PmlI 292 CACGTG CAC^GTG
306 CACGTG
SmlI 610 CTCAAG C^T[CT][GA]AG
796 CTCAAG
TspRI 15 CACTG CA[GC]TG[ACGT][ACGT]^
655 CACTG
#########################################
# 3 OR MORE CUT ENZYMES ( 47 enzymes) #
#########################################
AciI AfaI AluI AlwI BbvI
BmyI BsaAI BsaJI BsiYI BslI
BsmFI Bsp1286I BsrFI BssKI BstNI
BstYI CfoI Cfr10I Cfr13I DpnII
EcoRII Fnu4HI HaeIII HapII HhaI
HinP1I HinfI HpaII ItaI MaeII
MboI MflI MnlI MspA1I MspI
MvaI MwoI NdeII NlaIII NlaIV
PleI RsaI Sau3AI Sau96I ScrFI
TseI XhoII